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IFN-inducible p47 GTPases display differential responses to Schistosoma japonicum acute infection

Cellular & Molecular Immunology volume 7, pages 69–76 (2010)Cite this article

Abstract

Interferon gamma induced GTPase (IGTP) (also named Irgm3) and interferon gamma inducible protein 47 (IRG-47) (also named Irgd) are interferon (IFN)-inducible p47 GTPases that have been shown to regulate host resistance to intracellular pathogens. Little knowledge has been known about the role of p47 GTPases in host responses against extracellular pathogens. To investigate possible roles of IGTP and IRG-47 in the course of Schistosoma japonicum infection, IGTP and IRG-47 knockout and wild-type (WT) mice were challenged with cercariae of S. japonicum, and host responses were analyzed. At the acute stage of S. japonicum infection, mice that lacked IGTP displayed similar parasite burden and pathological damage to WT mice. Importantly, S. japonicum-infected IRG-47-deficient mice, in contrast to IGTP-deficient mice and WT mice, showed significantly reduced worms and lower egg-burden, but intense granulomatous reaction evoked by schistosome eggs in peripheral parts of liver lobes. In addition, upregulation of inflammation-related gene expression was observed in the spleen of IRG-47-deficient mice using oligonucleotide microarrays, in which multiple pathways of cytokine–cytokine receptor interaction, T-cell receptor signaling, complement, coagulation cascades and cell adhesion molecules were highlighted. Taken together, these data suggest that IGTP and IRG-47 might have distinct features that were differentially required for resistance to S. japonicum.

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Acknowledgements

We thank Dr Wenbin Huang (Nanjing First Municipal Hospital) for liver pathological analysis. This work was supported by the National Basic Research Program of China (973 Program 2007CB513106) and the National Science Foundation of China (NSFC) (Project No. 30430600 and No. 30872368).

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Author notes

  1. Xuesong Chen and Xiaotang Du: These authors contributed equally to this work.

  2. Dr MJ Ji or Dr GL Wu, Department of Pathogen Biology and Immunology, Nanjing Medical University, Nanjing 210029, China E-mail: jiminjun@njmu.edu.cn or glwu@njmu.edu.cn

Authors and Affiliations

  1. Department of Pathogen Biology and Immunology, Nanjing Medical University, Nanjing, China

    Xuesong Chen, Xiaotang Du, Meijuan Zhang, Donghui Zhang, Minjun Ji & Guanling Wu

  2. Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, China

    Donghui Zhang, Minjun Ji & Guanling Wu

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Chen, X., Du, X., Zhang, M. et al. IFN-inducible p47 GTPases display differential responses to Schistosoma japonicum acute infection. Cell Mol Immunol 7, 69–76 (2010). https://doi.org/10.1038/cmi.2009.100

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