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Establishment and Characterization of a Cell Based Artificial Antigen-Presenting Cell for Expansion and Activation of CD8+ T Cells Ex Vivo

Cellular and Molecular Immunology volume 5, pages 4753 (2008) | Download Citation

Abstract

Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL, which are ligands of co-stimulating receptors of CD28 and 4-1BB, respectively, were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3, named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8+ T cell activation, promoting CD8+ T cell proliferation, division, and long-term growth, inhibiting CD8+ T cell apoptosis, and enhancing CD8+ T cell secretion of IFN-γ and perforin. Furthermore, antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust, simple, reproducible and economical for expansion and activation of CD8+ T cells and may have important therapeutic implications for adoptive immunotherapy.

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Affiliations

  1. Department of Immunology, School of Medicine, Yangzhou University, Yangzhou 225001, China

    • Weijuan Gong
    • , Mingchun Ji
    • , Zhengfeng Cao
    • , Liheng Wang
    • , Yayun Qian
    • , Li Qian
    •  & Xingyuan Pan
  2. Testing Centre, Yangzhou University, Yangzhou 225001, China

    • Maozhi Hu

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Correspondence to Weijuan Gong.

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DOI

https://doi.org/10.1038/cmi.2008.6

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