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cAMP Modulates Macrophage Development by Suppressing M-CSF-Induced MAPKs Activation

Cellular & Molecular Immunology volume 5, pages 153–157 (2008)Cite this article

Abstract

M-CSF is a key cytokine in macrophage development by inducing MAPKs activation, and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development, the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by Western blotting assay, and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis. Macrophage morphology showed the successful establishment of the model of macrophage development. Western blotting assay revealed that M-CSF activated ERK, JNK and p38 in both mature and immature macrophages, and cAMP inhibited M-CSF-induced ERK, JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion, cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.

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Author notes

  1. Ning Zhu and Jian Cui: These authors contributed equally to this work

Authors and Affiliations

  1. Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, 100850, China

    Ning Zhu, Jian Cui, Chunxia Qiao, Yan Li, Jiyan Zhang & Beifen Shen

  2. Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng, China

    Ning Zhu, Yan Li, Yuanfang Ma & Beifen Shen

Authors
  1. Ning Zhu
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  2. Jian Cui
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  3. Chunxia Qiao
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  4. Yan Li
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  5. Yuanfang Ma
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  6. Jiyan Zhang
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  7. Beifen Shen
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Corresponding authors

Correspondence to Yuanfang Ma or Beifen Shen.

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Zhu, N., Cui, J., Qiao, C. et al. cAMP Modulates Macrophage Development by Suppressing M-CSF-Induced MAPKs Activation. Cell Mol Immunol 5, 153–157 (2008). https://doi.org/10.1038/cmi.2008.19

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  • DOI: https://doi.org/10.1038/cmi.2008.19

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