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Hypoxia-inducible factor 1 (HIF1) is a master transcription factor that regulates the expression of hypoxia-inducible genes involved in erythropoiesis, vascular remodeling and glucose metabolism in response to hypoxia. Dysregulation of HIF1α has been heavily implicated in tumor progression.1 In this study, using exome sequencing, we identified a synonymous somatic variant of HIF1α (c.1257A>G, E419E) in a 40-year-old patient with a primary malignant cardiac tumor. This variant is located in exon 10 of HIF1α and near the intron 9/exon 10 boundary. This mutation represents a novel form, and has no frequency record based on human cancer databases, including COSMIC, cBioPortal, as well as in dbSNP and 1000 Genomes. The minor allele frequency in the ExAC database is 3.533e−5 (4/113218). Bioinformatic predictions using SplicePort,2 GeneSplicer3 and Mutationtaster4 uniformly indicated that a new acceptor splice site would be created due to this single base exchange.
To confirm whether alternative splice transcripts are generated, a PCR primer pair was designed to amplify the cDNA fragment from exon 8 to exon 11. Two distinct DNA bands of different sizes were repeatedly observed in cardiac tumor tissue (Figure 1a). However, the lower band was markedly depleted in the tumor-adjacent tissue. Furthermore, we found that this lower band was undetectable in blood sample of patient and in normal heart tissues of spontaneous aborted fetuses (Supplementary Figure S1). Gel extraction and Sanger sequencing revealed that the lower band (373 bp) lacked exon 10 when compared to the upper band (659 bp). In this alternative splice variant, exon 9 and 11 were directly joined, and the altered reading frame was immediately terminated due to two sequential stop codons at presumptive amino acid (aa) positions 418 and 419. This truncated 417-aa variant lacked most of important domains. Thus, we do not expect that this splice variant would exhibit normal transcriptional activity.
Nuclear translocation is fundamental for transcriptional activation. To compare the nuclear location of full-length and the 417-aa HIF1α in vitro, subcellular localization and nuclear extraction assays were performed in human HeLa and 293T cells, respectively. Both confocal microscopy and western blot results indicated that the 417-aa HIF1α was mostly retained in the cytoplasm, whereas full-length HIF1α was translocated to the nucleus.(Figure 1b). However, HIF1α 417-aa displayed higher stability in 293T cells due to lack of Pro-564 residue acid, which is essential for proline hydroxylation and VHL-dependent E3 degradation (Supplementary Figure S2). As a consequence, expression of classical HIF1 downstream targets, including VEGFA and VEGFB, as well as PHD2 and PHD3 were downregulated in cardiac tumor tissues. Next, we performed RNA-seq to compare the transcriptomes of full-length and 417-aa HIF1α in 293T cells. Especially, PAK6 (p21 protein activated kinase 6) was upregulated by full-length HIF1α but was markedly downregulated in cells transfected with 417-aa HIF1α (Figure 1c). A previous study found that PAK1 upregulates HIF1α in colorectal cancer.5 Whether PAK6 acts as a novel interaction partner of HIF1α requires further investigation.
Different alternative transcripts including HIF1α-827, 785, 736, 557, 516 and 417 have been reported.6, 7, 8 however, the roles of these splice variants in human diseases are still unclear. Lee et al.6 claimed that HIF1α-417, the shortest splice variant, could undergo nuclear translocation and promote transactivation using the transactivation domain of HIF1β; however, in our study, both nuclear translocation and transactivation were, at least in part, deficient with this splice variant.
Although our results fail to provide a solid correlation between HIF1α (c.1257A>G, E419E) and the premature HIF1α transcript, we found that the 417-aa HIF1α owns higher stability and is deficient with normal nuclear translocation. We postulated that this variant (c.1257A>G) enables HIF1α alternative splicing to compromise excessive HIF1α transcription during the early stages of tumorigenesis.
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Acknowledgements
This work was supported by 973 Program Grant 2013CB945403, National Natural Science Foundation of China (81430005 and 81601283) and China Postdoctoral Science Foundation (KLH1322083).
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Zhou, X., Zeng, W., Peng, R. et al. A hypoxia-inducible factor 1α null splice variant lacking exon 10. Cell Death Dis 8, e2873 (2017). https://doi.org/10.1038/cddis.2017.269
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DOI: https://doi.org/10.1038/cddis.2017.269
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