Although cardiac cytosolic cyclic 3′,5′-adenosine monophosphate (cAMP) regulates multiple processes, such as beating, contractility, metabolism and apoptosis, little is known yet on the role of this second messenger within cardiac mitochondria. Using cellular and subcellular approaches, we demonstrate here the local expression of several actors of cAMP signaling within cardiac mitochondria, namely a truncated form of soluble AC (sACt) and the exchange protein directly activated by cAMP 1 (Epac1), and show a protective role for sACt against cell death, apoptosis as well as necrosis in primary cardiomyocytes. Upon stimulation with bicarbonate (HCO3−) and Ca2+, sACt produces cAMP, which in turn stimulates oxygen consumption, increases the mitochondrial membrane potential (ΔΨm) and ATP production. cAMP is rate limiting for matrix Ca2+ entry via Epac1 and the mitochondrial calcium uniporter and, as a consequence, prevents mitochondrial permeability transition (MPT). The mitochondrial cAMP effects involve neither protein kinase A, Epac2 nor the mitochondrial Na+/Ca2+ exchanger. In addition, in mitochondria isolated from failing rat hearts, stimulation of the mitochondrial cAMP pathway by HCO3− rescued the sensitization of mitochondria to Ca2+-induced MPT. Thus, our study identifies a link between mitochondrial cAMP, mitochondrial metabolism and cell death in the heart, which is independent of cytosolic cAMP signaling. Our results might have implications for therapeutic prevention of cell death in cardiac pathologies.
Mitochondria are involved in cell life and fate decision through their multiple biological functions in energetic metabolism, reactive oxygen species (ROS) detoxification and cell death.1, 2, 3 These functions are crucially regulated to provide sufficient energy for cell functions, maintain mitochondrial membrane integrity and avoid excessive cell death.4, 5 Moreover, mitochondria may participate in Ca2+ homeostasis via matrix Ca2+ accumulation through the mitochondrial Ca2+ uniporter (MCU), Ca2+ release into the cytosol and propagation to other mitochondria, notably in excitable cells.6, 7, 8 In cardiomyocytes, intracellular Ca2+ movements are crucial for proper myofibril contraction and relaxation and energetic metabolism. Moreover, recent studies in cardiomyocyte-specific mutant mouse lacking the MCU showed a link between mitochondrial Ca2+ uptake and energetic supply in relation with cardiac workload during acute stress.9, 10 In contrast, excessive mitochondrial Ca2+ accumulation, ROS production and adenine nucleotide depletion result in the sudden opening of a megachannel, namely the permeability transition pore complex. The prolonged opening of this unspecific pore leads to the mitochondrial permeability transition (MPT), cell death, inflammation and irreversible tissue damage.11, 12 MPT can be a critical event in severe cardiac diseases such as ischemia–reperfusion injury and heart failure (HF) as well a radiation-induced cardiotoxicity.11, 13, 14 Hence, MPT inhibition by cyclosporin A (CsA) has been shown to limit cardiac damages and improve cell survival. Inhibition of MPT has thus become an attractive therapeutic strategy in cardioprotection.15
Cyclic 3′,5′-adenosine monophosphate (cAMP) is a major second messenger in many organs, particularly in the heart, where it regulates diverse physiological processes such as Ca2+ homeostasis, beating frequency and myocardial contractility as well as cell death.16 In the working myocardium, cAMP can activate protein kinase A (PKA) and/or the exchange protein directly activated by cAMP (Epac) to mediate diverse biological effects, including cardiac remodeling and hypertrophy.17, 18, 19, 20, 21, 22 In addition to tmACs, cAMP can also be generated by soluble adenylyl cyclase (sAC), which is not regulated by heterotrimeric G proteins or forskolin (FSK), but can be activated by bicarbonate (HCO3−) and Ca2+.16, 23, 24 sAC was found inside mitochondria in the brain and liver and in certain mammalian cell types.25, 26, 27, 28, 29 In the liver and brain, in response to HCO3− and/or Ca2+, mitochondrial cAMP stimulates oxidative phosphorylation and ATP production.30 In coronary endothelial cells, HCO3− indirectly modulates the cell fate through apoptosis.31, 32 As a result, this pathway serves as a mechanism for metabolic adaptation to mitochondrial dysfunction and could be a potential novel target to treat genetic mitochondrial diseases.33 Altogether, these findings suggest that mitochondrial sAC functions as a metabolic sensor to stimulate mitochondrial biological functions. If proven in primary cardiomyocytes, this intramitochondrial cAMP pathway might have clinical implication in HF as patients diagnosed with HF have markedly impaired mitochondrial metabolism and cAMP signaling, both contributing to cardiomyocyte dysfunction.16, 34
Intrigued by these previous findings, we tested the existence of a cAMP mitochondrial pathway in differentiated adult and neonatal cardiomyocytes and observed that activation of this pathway prevents various cell deaths. Our results also show that cardiac mitochondria isolated from adult rat hearts contain a truncated form of sAC (sACt) as a source of cAMP as well as Epac1. A role of this local pathway is to control mitochondrial Ca2+ entry through the MCU and to prevent the deleterious consequences of mitochondrial Ca2+ overload such as dissipation of mitochondrial membrane potential (ΔΨm) and induction of MPT. Interestingly, this mitochondrial sACt-Epac1-MCU pathway remains functional in a rat model of HF induced by aortic stenosis and its activation prevents MPT.
Mitochondrial cAMP prevents cardiac cell death, apoptosis as well as necrosis
To evaluate the capacity of sAC and cAMP to regulate the cardiomyocyte cell fate, we infected primary neonatal cardiomyocytes with two adenoviruses to overexpress the full-length sAC (sACfl) and the sACt, respectively, 24 h before cell death induction by three different cell death inducers, that is, camptothecin, H2O2 and TNF-α+actinomycin D. We showed that the stimulation of endogenous sAC with HCO3− as well as overexpression of sACt prevented the various cell death modalities, apoptosis as well as necrosis measured by annexin/7-AAD labeling (Figures 1a and b). In contrast, inhibition of sAC with 2-hydroxyestradiol (2HE), a sAC inhibitor,29 aggravated significantly cell deaths (Figure 1a). We observed also that cAMP protects from nuclei alterations measured by counting Hoechst-stained nuclei exhibiting morphologic and biochemical alterations, that is, pycnosis and karryorrhexis (Figures 1c–f).
Mitochondrial sAC produces locally cAMP and regulates ΔΨm upon calcium overload
As mitochondria may be impermeant to cytosolic cAMP,35 we constructed an adenovirus encoding a cAMP-sensitive fluorescence resonance energy transfer (FRET) sensor (Epac-SH187)36 fused with a 4mt sequence and infected rat neonatal cardiomyocytes with this sensor, 4mt-Epac-SH187. The localization of 4mt-Epac-SH187 in mitochondria was shown by colocalization of its green fluorescence with mitotracker red fluorescence (Pearson's coefficient: 0.92±0.02, n=6) (Figure 2a). Following infection with 4mt-Epac-SH187, we sequentially treated the cells with HCO3− to activate sAC, FSK to activate tmAC and 8-CPT-2′-OMe-cAMP-AM (8CPT-cAMP AM), a permeant cAMP analog, to directly activate the sensor (Figure 2b). Addition of HCO3− induced an increase in cAMP measured by 4mt-Epac-SH187 (Figure 2b), which was virtually absent when the cells were infected with the cytoplasmic cAMP sensor Epac-SH187 (Figure 2c). On the contrary, FSK induced a large response of cAMP measured with the cytoplasmic sensor (Figure 2c) and a smaller response of cAMP measured with the mitochondrial sensor (Figure 2b). These results are compatible with HCO3−-activating sAC in mitochondria and FSK increasing cAMP in the cytoplasm by activating tmAC. The small response to FSK observed with 4mt-Epac-SH187 might be due to incomplete targeting of the probe to mitochondria. Of note, 4mt-Epac-SH187 has a much higher dynamic range than previously published sensors such as mito-EpacH90,35 so that even a small expression of the probe in the cytosol would lead to a detectable signal. Alternatively, the small response to FSK measured with 4mt-Epac-SH187 might be due to Ca2+ stimulation of sAC in the matrix upon FSK stimulation as suggested previously,29 or to a small permeability of the mitochondrial inner membrane (IM) to cAMP. Interestingly, 2HE totally prevented the mitochondrial cAMP increase elicited by HCO3− (Figure 2d). This confirms the involvement of a mitochondrial sAC as a source of cAMP in cardiomyocytes.
To address the role of sAC in the regulation of mitochondrial function, cardiomyocytes were transfected with siRNA control and siRNA against sAC for 48 h. Next, they were loaded with the fluorescent ΔΨm indicator, TMRM, permeabilized and treated with Ca2+. The decrease in the level of sAC did not induce any changes of the mitochondrial network (Figures 2e and f). However, the measure of the TMRM fluorescence ratio showed that silencing of sAC markedly aggravated the loss of ΔΨm induced by Ca2+, suggesting a role of cAMP in ΔΨm control in stress conditions.
cAMP is produced by sACt in isolated mitochondria
Mitochondria were isolated from rat heart ventricles by differential centrifugation and extensive washes.37 First, we analyzed their morphology by transmission electron microscopy (Figure 3a) and their purity by western blotting (Figure 3b). As expected, mitochondria appeared round-shaped (mean diameter, 0.8 μm) and presented numerous cristae, compatible with a high respiratory capacity. In comparison with rat ventricles homogenate (H), isolated mitochondria (M) were enriched in the adenine nucleotide translocase (ANT), an IM protein and almost not contaminated by cytosolic proteins such as GAPDH, myofibrillar proteins such as troponin I (TnI) and sarcoplasmic reticulum-associated proteins such as phospholamban (PLB) (Figure 3b). Using specific monoclonal antibodies, we detected the sACt (48 kDa) and the sACfl (187 kDa) in H fraction, whereas only the short form, which is the active form,38 was found in the mitochondria preparation (Figure 3b). Next, we measured cAMP production in freshly isolated mitochondria. We observed that HCO3− and also, to a lesser extent, Ca2+-stimulated cAMP production in a dose-dependent manner and potentiated the response to ADP (Figure 3c). Although a small stimulatory effect of Ca2+ on cAMP production was observed at 0.1 μM, when increasing the concentration to 10 μM, mitochondria lost their membrane potential (not shown), lowering markedly cAMP production (Figure 3c). Ca2+ effects were abolished in the presence of RU360, confirming that the effect on cAMP levels is due to a specific uptake of Ca2+ within the matrix. Similarly, when mitochondria were depolarized by the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP), no cAMP production was detected even in the presence of HCO3− (Figure 3d). Moreover, 2HE reduced basal and fully blocked HCO3−-stimulated cAMP production (Figure 3d). As a control, FSK had no stimulatory effect on cAMP in isolated mitochondria, confirming clearly the absence of tmAC within mitochondria (Figure 3d).
cAMP increases ΔΨm, respiration and ATP levels
Next, the ΔΨm was montored with the probe, rhodamine 123 (Rhod123), in the presence of various respiratory substrates (Figures 3e and f). We used 8Br-cAMP, a membrane-permeant cAMP analog, as a control, and HCO3− to stimulate endogenous production of cAMP. Figure 3e shows that 8Br-cAMP slightly hyperpolarized mitochondria in condition of complex I-driven respiration, but failed to have any effect in the presence of respiratory substrates for complexes II–IV. In contrast, HCO3− triggered a hyperpolarization in all conditions of substrates (Figure 3f). This hyperpolarization was accompanied by an increase in oxygen consumption in response to HCO3− (Figure 4a). Finally, when mitochondria were stimulated by HCO3− or Ca2+, this led to an increase in ATP production both in the absence and presence of ADP (Figure 4b). These data indicate that cAMP produced by a mitochondrial sAC stimulates the oxidative phosphorylation increasing ΔΨm and mitochondrial ATP synthesis.
cAMP delays Ca2+-induced MPT
We hypothesized that the cyclic nucleotide could have a role in the regulation of MPT.39 In isolated cardiac mitochondria, MPT can be elicited by 10 μM Ca2+ and prevented by 5 μM CsA and detected as a loss of ΔΨm and a matrix swelling.37 We used two robust miniaturized assays37, 40 to concomitantly measure the effect of sAC inhibition by 25 μM 2HE on mitochondrial depolarization (Figures 4c and d) and matrix swelling (Figures 4e and f) induced by 10 μM Ca2+. sAC inhibition by 2HE accelerated the depolarization (Figures 4c and d) and swelling (Figures 4e and f) induced by Ca2+, as shown by the decreased half-time of ΔΨm loss and swelling (Figures 4d and f). Conversely, 15 mM HCO3− slowed both processes (Figures 4d and f), suggesting that cAMP elevation confers a protection of mitochondria from Ca2+-induced MPT.
Mitochondrial cAMP effects are independent of PKA
cAMP effects are classically mediated by activation of two main effectors, PKA and Epac to regulate a plethora of biological functions in the heart.19 In mitochondria, PKA has been reported to be associated with outer membrane (OM) or to be in the matrix for controlling mitochondrial dynamics and oxidative metabolism.35,41,42 We thus examined whether PKA was involved in the mitochondrial cAMP effects by testing the effects of two different pharmacological PKA inhibitors, H89 and KT5720, on the induction of MPT by Ca2+. As shown in Supplementary Figure 1, these inhibitors had no significant effect on ΔΨm and swelling, indicating that PKA may not be involved in MPT regulation.
Epac1 mediates cAMP effect on respiration and MPT
Then, we checked the expression of Epac isoforms. As shown in Figure 5a, both Epac1 and Epac2 isoforms were found in isolated cardiac mitochondria as well as in mitoplasts generated by osmotic shock, but were absent in the postmitoplast supernatant. This suggests that Epac can be anchored to the IM facing the mitochondrial matrix or the intermembrane space or localized in the matrix.
To evaluate the functional role of Epac, we used three pharmacological Epac inhibitors exhibiting different specificities and tested their effects on Ca2+-induced depolarization and swelling as well as oxygen consumption. We used ESI09, a pan-Epac inhibitor, ESI05, an Epac2-selective inhibitor,21, 43 and CE3F4, an Epac1-selective inhibitor.44 As shown in Figure 5b, Epac1 inhibition with 50 μM CE3F4 decreased basal oxygen consumption and also prevented the stimulatory effect of HCO3−. Moreover, CE3F4 accelerated Ca2+-induced depolarization (Figures 5c and d) and swelling (Figures 5e and f). Similar findings were obtained with ESI09 but not with ESI05 (Supplementary Figure 2). These data thus point to Epac1 as a key effector in mitochondrial cAMP effects.
Next, we tested the effect of Epac1 on the level of matrix Ca2+ using the Rhod-2 probe, and CGP37157, a mitochondrial Na+/Ca2+ exchanger (mNCX) inhibitor, appeared to accelerate Ca2+ entry in isolated mitochondria (Figures 6c and d). This effect was similar to that of CE3F4. However, the combination of both inhibitors produced an additive effect, suggesting that they act via two distinct mechanisms. Thus, it is unlikely that Epac1 regulates mNCX. To examine the role of MCU, we used RU360, a highly specific MCU inhibitor. As anticipated, RU360 (from 0.2 to 1 nM) induced a dose-dependent inhibition of Ca2+ entry (Supplementary Figure 3). Interestingly, inhibition of Epac in the presence of non-maximal concentrations of RU360 partially restored Ca2+ entry within mitochondria (Figures 6e and f and Supplementary Figure 3), but this effect was abrogated when the MCU was fully inhibited with 1 nM RU360 (Supplementary Figure 3a). These results suggest that MCU is the major effector of Epac1 for the regulation of mitochondrial Ca2+ movements.
Epac1 mediates mitochondrial Ca2+accumulation and ΔΨm loss in cardiomyocytes
The Epac1 silencing by siRNA indicated that a decreased level of Epac1 in neonatal rats decreased ΔΨm (Figures 7a and c) and in parallel accelarated the mitochondrial calcium entry (Figures 7d and e), as does the inhibitor CE3F4 in adult permeabilized cardiomyocytes upon addition of Ca2+ (Figures 7f and g). At this concentration, Ca2+ did not affect ΔΨm, avoiding any artifact since most ions and metabolites transports are dependent of the ΔΨm (Supplementary Figures 4a and b). We also checked that our conditions of fluorescence excitation did not trigger MPT (Supplementary Figures 4c and d). Altogether, these results suggest that Epac1 has a role in reducing the entry of Ca2+ in mitochondria, and then indirectly stabilizes the ΔΨm in primary cardiomyocytes.
The mitochondrial cAMP pathway can prevent MPT in HF rat model
To evaluate the ability of the mitochondrial cAMP pathway to regulate MPT in a pathological model, we induced HF in rats by transverse aortic constriction (TAC) during 22 weeks.45 As shown in Supplementary Figure 5a, TAC rats showed a strong cardiac and lung hypertrophy. Accordingly, cardiac function and the fractional shortening of the left ventrice were diminished (Supplementary Figures 5b and c). Expression level of various proteins was analyzed in heart ventricle homogenates and mitochondrial fraction. As shown in Figures 8a and b, sACt protein expression was reduced and Epac1 expression was increased in homogenate and mitochondria from HF as compared with sham hearts. MCU expression was similar in mitochondrial fraction from HF and sham rats. To explore how HF affects mitochondrial Ca2+-induced MPT, Ca2+-induced mitochondrial depolarization and Ca2+ accumulation was measured in isolated mitochondria from HF and sham rats. As shown in Figures 8c and d and Supplementary Figures 6a–d, Ca2+ induced a faster depolarization and Ca2+ uptake in HF than in sham mitochondria. In line with this, Ca2+ induced a faster mitochondrial swelling in HF than in sham mitochondria (Figure 8e and Supplementary Figures 6e and f). This confirms that MPT is altered in HF, which could make mitochondria more vulnerable to Ca2+ overload.46 Interestingly, mitochondria from HF rats still responded to HCO3− stimulation of mitochondrial cAMP production by sAC, by delaying ΔΨm loss, Ca2+ entry and MPT (i.e. matrix swelling). These effects were blunted by sAC or Epac1 inhibition with CE3F4 (Figures 8c–e and Supplementary Figures 6a–f).
In this study, we characterized a functional cAMP pathway within the mitochondria of neonatal and adult cardiomyocytes, which can regulate mitochondrial function and cell death. cAMP is locally produced within the mitochondria by a Ca2+/HCO3−-sensitive sACt and activates Epac1 to stimulate oxidative metabolism while preventing MPT by limiting mitochondrial Ca2+ accumulation via MCU. As HCO3− production can be catalyzed by carbonic anhydrase from CO2 and H2O, CO2 being produced by the Krebs cycle and the pyruvate deshydrogenase inside mitochondrial matrix, our data thus link, for the first time, mitochondrial metabolism, cAMP and cell death in the heart, independently of cytosolic cAMP signaling.
Our data are in good agreement with pioneer studies revealing the existence of a mitochondrial cAMP signaling in various cell types.27, 28, 29 Prompted by the observation that a G-protein- and FSK-insensitive sAC is present in various organelles,38, 47, 48, 49 Acin-Perez et al.27 discovered a CO2-HCO3−-sAC-cAMP-PKA (mito-sAC) signaling cascade entirely contained within the mitochondria. This mito-sAC cascade serves as a metabolic sensor modulating ATP generation and ROS production in response to nutrient availability.29 By targeting the recently developed Epac-SH187 cAMP FRET sensor36 to the mitochondria, we showed that sAC activation by HCO3− increases mitochondrial cAMP in neonatal cardiomyocytes, as shown earlier in HeLa and CHO cells.35 We showed that the constitutive mitochondrial cAMP signaling pathway regulates ΔΨm and MPT not only in healthy but also in failing heart mitochondria and that these functions are mediated by Epac1.
A functional mito-sAC pathway in mitochondria from adult heart
Although it was already known that sAC can be localized into mitochondria,47, 48, 49 little was known about their biological function in the organelle. Here, we identified endogenous sACt in cardiac mitochondria and mitoplasts. We showed for the first time that increasing intramitochondrial cAMP level delays the onset of MPT, while stimulating oxygen consumption. Although HCO3− and Ca2+ enhanced cAMP production, HCO3− was more potent than Ca2+, which is in line with the fact that HCO3− and Ca2+ stimulatory effects are not redundant: HCO3− modulates the active site of sAC, whereas Ca2+ increases ATP affinity.27 Interestingly, a specific inhibitor of sAC, 2HE, totally prevented the effects of HCO3− and Ca2+, indicating that sAC may be the unique source of mitochondrial cAMP.
Effectors of mitochondrial cAMP
While PKA is the canonical mediator of cAMP in a number of cell functions and cell subcompartments, and was shown earlier to regulate mitochondrial ATP and ROS production,27, 29, 50, 51 PKA was clearly not involved in the induction of MPT by Ca2+ as H89 and KT5720 failed to modulate it. We thus focused our interest on Epac, because it emerged in the past decade as another important player in cAMP signaling.20 Although Epac possesses a mitochondrial-targeting sequence at its N terminus and has been shown to be localized inside mitochondria by heterologous expression,52 to our knowledge there has been no report on a role for this protein in mitochondrial function. Although the Epac2-selective inhibitor ESI05 had no effect, the non-selective inhibitor ESI09 or the Epac1-selective inhibitor CE3F4 antagonized the induction of MPT by Ca2+. This indicates that Epac1 but not Epac2 is involved in the regulation of MPT. We found also that CE3F4 inhibits oxygen consumption. As the efficiency of CE3F4 to regulate oxygen consumption with a better efficiency than MPT, we speculate that Epac1 could have several targets, which remain to be identified, regulating differentially various mitochondrial functions.
In neonatal rat cardiomyoctes, silencing of Epac1 modulated the Ca2+ entry and the ΔΨm. In the heart, Epac1 was recently shown to be localized and functionally involved also in nuclear signaling, whereas Epac2 is located at the T tubules and regulates arrhythmogenic sarcoplasmic reticulum Ca2+ leak.53 While the intermediate downstream effector(s) of mitochondrial Epac1 still need to be identified, our results indicate that Epac1 activation may inhibit MCU activity. This hypothesis is supported by the fact that inhibition of MCU, but not of mNCX, mimics the effects of mitochondrial cAMP elevation in preventing MPT. Thus, we propose that activation of mitochondrial Epac1 protects the organelle from Ca2+ overload and from subsequent MPT via MCU modulation.
Possible implications of the mitochondrial cAMP pathway for cell death and cardioprotection
Ca2+ overload is considered as a conserved inducer of regulated cell death modalities.54 Using modulation of sAC by genetic and pharmacological manipulations in primary cardiomyocytes, our study demonstrates for the first time that activation of the mitochondrial cAMP pathway exerts an inhibition on MPT in vitro and on various cell death modalities, that is, extrinsic and intrinsic apoptosis as well as necrosis. Conversely, pharmacological inhibition of sAC increased markedly nuclear damage and cell death. Thus, the targeted activation of this mitochondrial cAMP pathway may preserve cardiomyocytes from mitochondrial Ca2+ overload and cell death in vivo. In that respect, in a pathological rat model of HF induced by pressure overload, which goes along with strong cardiac hypertrophy, cardiac function alteration, tissue remodeling, bioenergetic alterations and cardiomyocyte cell death,45, 46 sACt is downregulated and Epac1 is upregulated in mitochondria. However, the increase in Epac1 did not compensate the decrease of sACt in terms of function, suggesting that the level of cAMP is limiting for Epac1 in the control of MPT in cardiac mitochondria. Moreover, we found that the MPT alterations can be alleviated by stimulation of the mitochondrial cAMP pathway. Thus, this new mitochondrial cAMP/sACt/Epac1/MCU pathway might have therapeutic implications to regulate cell death in cardiac pathologies, such as HF and/or myocardial infarction.54, 55
Material and Methods
Unless specified, all reagents and chemicals are from Sigma-Aldrich (Saint-Quentin Fallavier, France) and of analytical grade.
All animal care and experimental procedures conformed to the European Community guiding principles in the care and use of animals (Directive 2010/63/EU of the European Parliament) and authorizations to perform animal experiments according to this decree were obtained from the French Ministry of Agriculture, Fisheries and Food (No. D-92-283, 13 December 2012). All studies involving rats are reported in accordance with the ARRIVE guidelines for reporting experiments involving animals.56 A total of 60 healthy, 4 sham and 4 HF rats were used in the experiments described here.
Surgical procedure and echocardiography
Male Wistar rats at 3 weeks of age (60–70 g; Janvier, Le Genest St Isle, France) were anesthetized with pentobarbital (60 mg/kg). The thoracic cage was opened and a stainless-steel hemoclip was placed on the ascending aorta, to promote HF after 22 weeks, as described previously.57 Sham-operated animals were used as controls. Cardiac structure and function was evaluated by echocardiograph. Cardiac and pulmonary hypertrophy was determined as a ratio of organ weight to tibia length and to body weight.57 Transthoracic two-dimensional-guided M-mode echocardiography of rats was performed using an echocardiograph with a 15 MHz linear transducer (Vivid 9; General Electric Healthcare, Vélizy Villacoublay, France) under 3% isoflurane gas anesthesia and fractional release was calculated as described.57
Isolation of cardiac mitochondria
Mitochondria were isolated from the heart of adult male Wistar rats at 8–10 weeks of age (275–375 g; Janvier) as described.37 Briefly, the heart was rapidly removed and placed into a cold buffer containing 0.3 M sucrose, 0.2 mM EGTA and 5 mM TES (pH 7.2). The heart was grinded with Polytron fastly and homogenized by using the Potter. The homogenate was centrifuged at 500 × g for 10 min at 4 °C. Then, the supernatant was carefully removed and centrifuged again at 3000 × g for 10 min at 4 °C. The pellets were washed in the isolation buffer and the mitochondria were kept on ice until use within 3 h.
Mitochondrial transmembrane potential and swelling in isolated mitochondria
Isolated mitochondria (25 μg proteins) were incubated with Ca2+ and drugs in 96-well microtiter plates.37 ΔΨm was measured using the fluorescent probe, Rhod123 (excitation=485 nm and emission=535 nm; Enzo Life Sciences, Villeurbanne, France) in a buffer containing 200 mM sucrose, 10 mM MOPS, 10 μM EGTA, 1 mM H3PO4, 5 mM succinate and 2 μM rotenone (pH 7.4) using Tecan Infinite 200 spectrofluorimeter (Tecan, Männedorf, Switzerland). In parallel, matrix swelling was measured via light absorbance at 540 nm.37
Isolated mitochondria (50 μg proteins) were incubated with drugs in a buffer containing 250 mM sucrose, 30 mM K2HPO4, 1 mM EGTA, 5 mM MgCl2, 15 mM KCl and 1 mg/ml bovine serum albumin (BSA) (pH 7.4) supplemented with respiratory substrates and MitoXpress, an oxygen-sensitive phosphorescent dye (LUXCEL, Cork, Ireland). Oxygen consumption was measured in real time for 60 min at 30 °C in 96-well plates using Tecan Infinite 200 (excitation=380 nm and emission=650 nm) in the presence of 1.65 mM ADP and with 5 mM malate and 12.5 mM glutamate.37
Mitochondrial Ca2+ uptake in isolated mitochondria
Isolated mitochondria (25 μg proteins) were incubated with 5 μM Rhod-2 (Enzo Life Sciences) in the buffer containing 200 mM sucrose, 10 mM MOPS, 10 μM EGTA, 1 mM H3PO4, 5 mM succinate and 2 μM rotenone for 30 min in dark at room temperature. Afterwards, the mitochondria were washed two times. Then, the mitochondria were treated with various drugs for 10 min before applying Ca2+. Fluorescence was measured in real time for 60 min at room temperature in 96-well plates using Tecan Infinite 200 (excitation=552 nm and emission=581 nm).
cAMP measurements by ELISA
cAMP measurements were performed according to the manufacturer’s instructions using monoclonal anti-cAMP antibody-based direct cAMP ELISA Kit (New East Biosciences, King of Prussia, PA, USA) on freshly isolated mitochondria from rat hearts (500 μg proteins per sample) treated or not by HCO3−, Ca2+ and Ca2++Ru360 for 20 min at room temperature before centrifugation and lysis. The sensitivity of cAMP detection is 29.6 fmol/ml.57, 58
ATP measurements in isolated mitochondria were performed according to manufacturer’s instructions using ATP Bioluminescence Assay Kit CLSII (Roche, Basel, Switzerland).
Total mitochondrial proteins were resolved on 4–15% Tris-glycine SDS-PAGE gels and electroblotted onto polyvinylidene fluoride membranes (Bio-Rad, Marnes La Coquette, France). Following electrotransfer, membranes were blocked for 1 h at room temperature in 5% BSA-PBST (10 mM Tris-HCl, pH 8.0/150 mM NaCl/0.1% Tween-20). Next, membranes were incubated overnight at 4 °C with primary antibody. The day after, the membranes were washed six times with PBST and incubated with peroxidase-conjugated secondary antibody at room temperature for 1 h. Peroxidase activity was detected with enhanced chemiluminescence (ECL Advance Western Blotting Detection Kit; Thermo Scientific, Villebon sur Yvette, France). For protein detection, the following antibodies were used: sAC (Abcam Cambridge, UK; CEP Biotech, Tamarac, FL, USA), Epac1, 2 (Cell Signaling, Danvers, MA, USA), ANT (Abcam), GAPDH (Cell Signaling), VDAC (Genosphere, Paris, France), TnI (Cell Signaling), PLB (Cell Signaling) and MCU (Biorbyt, Berkeley, CA, USA).
Construction of mitochondria-targeted FRET sensor for cAMP
The mitochondrial-targeting sequence 4mt, encoding four copies of the signal sequence from subunit VIII of human cytochrome C oxidase, was amplified using the Advantage Polymerase (Clontech, Mountain View, CA, USA) and primers F, 5′-ACTATAGGGAGACCCAAGCTTATG-3′ and R, 5′-TGGTGGCGGCAAGCTTCTTGCTCACCATGGTGGC-3′. The pcDNA-4mt-D3-cpv vector used as a matrix for amplification of 4mt was a kind gift from Dr. Roger Tsien (HHMI investigator at the University of California San Diego, San Diego, CA, USA). The PCR fragment was cloned into the HindIII restriction site of pcDNA3-Epac-SH187 using the Infusion HD Cloning System (Clontech). Epac-SH187 encodes for a fourth-generation Epac1-based cAMP sensor and was a kind gift from Dr. Kees Jalink (The Netherlands Cancer Institute, Amsterdam, Netherlands).36 Once the pcDNA-4mt-Epac-SH187 vector was amplified in Stellar Escherichia coli (Clontech) bacteria, its identity with parental sequences was verified by PCR using primers F, 5′-ACTCACTATAGGGAGACC-3′ and R, 5′-TGCGGCCGCCATGGTGGC-3′, and DNA double-strand sequencing (INSERM U1056 – UMR 5165 CNRS UPS – UDEAR, Toulouse, France). Adenoviruses encoding Epac-SH187 and 4mt-Epac-SH187 were generated by Welgen Inc (Worcester, MA, USA).
Cardiomyocyte isolation, adenoviral infection and cell death evaluation
Adult and neonatal cardiomyocytes were isolated as described previously.59,60 For FRET experiments, neonatal cardiomyocytes were plated on 35-mm, laminin-coated culture dishes (10 μg/ml) at a density of 4 × 105 cells per dish. The day after, cells were infected with Epac-SH187 and 4mt-Epac-SH187 adenoviruses in Opti-MEM (Life Technologies, St Aubin, France) for 48 h. Similarly, adenoviruses expressing sACt and sACfl were used (generous gift from Pr. M Conti, University of California, San Francisco, CA, USA). For confocal microscopy experiments, adult cardiomyocytes were plated on 35-mm, laminin-coated culture dishes (10 μg/ml) at a density of 2 × 104 cells per dish. For cell death evaluation, neonatal cells were stained with Apoptosis/Necrosis Detection Kit (Abcam) for 1 h at room temperature as described by the manufacturer.
siRNA transfection to knockdown sAC and Epac1
On-Target plus SMART pool siRNA, a mixture of four siRNA provided as a single reagent were purchased from Dharmacon (Lafayette, CO, USA). At day 0, neonatal cardiomyocytes were plated overnight on 35-mm, laminin-coated culture dishes (10 μg/ml) at 4 × 105. At day 1, the cells were transfected with 50 nM sAC/Epac1 or non-targeting control siRNA using Lipofectamine RNAi MAX Transfection Reagent (ThermoScientific, Waltham, MA, USA) for 48 h.
Mitochondrial transmembrane potential measurement in neonatal cardiomyocytes
Isolated rat cardiomyocytes were loaded with 100 nM TMRM at 37 °C for 15 min. Afterwards, the sarcolemmal membrane was permeabilized by perfusion of digitonin (5 μg/ml) in a Ca2+-free internal solution that contained 50 mM KCl, 80 mM potassium aspartate, 4 mM sodium pyruvate, 20 mM HEPES, 3 mM MgCl2, 3 mM Na2ATP, 5.8 mM glucose and 0.5 mM EGTA (pH 7.3 with KOH). Then, the free Ca2+ concentration in the internal solution was increased to 200 nM. The Ca2+ was calculated using the Maxchelator program from the Stanford University (Stanford, CA, USA). Images were acquired with a Leica (SP5) confocal microscope (Mannheim, Germany). Excitation was achieved by a white light laser fitted at 549 nm and emission collected at 570 nm. Analyses were made with Image J program (Wayne Rasband, National Institutes of Health, USA).
Measurement of mitochondrial Ca2+ in cardiomyocytes
Isolated neonatal or adult rat cardiomyocytes were loaded with 5 μM Rhod-2 at 37 °C for 30 min. To remove cytosolic Rhod-2, the sarcolemmal membrane was permeabilized by perfusion of digitonin (5 μg/ml) in a Ca2+-free internal solution that contained 50 mM KCl, 80 mM potassium aspartate, 4 mM sodium pyruvate, 20 mM HEPES, 3 mM MgCl2, 3 mM Na2ATP, 5.8 mM glucose and 0.5 mM EGTA (pH 7.3 with KOH). After the sarcolemmal membrane was permeabilized, the free Ca2+ concentration in the internal solution was increased to 200 nM. The Ca2+ was calculated using the Maxchelator program from Stanford University. Images were acquired with a Leica (SP5) confocal microscope. Excitation was achieved by a white light laser fitted at 552 nm and emission collected at 575 nm. Analyses were made with Image J program.
FRET measurements of cAMP levels
FRET imaging experiments were performed 48 h after infection of neonatal cardiomyocytes. Cells were bathed in Hepes-buffered Ringer’s solution containing: 125 mM NaCl, 25 mM HEPES, 10 mM glucose, 5 mM K2HPO4, 1 mM MgSO4 and 1 mM CaCl2, pH 7.4. For sAC activation by HCO3−, the medium was the Krebs–Henseleit solution containing: 120 mM NaCl, 2.09 mM K2HPO4, 0.34 mM KH2PO4, 24 mM NaHCO3, 1 mM MgSO4, 1 mM CaCl2 and 10 mM d-glucose. Krebs–Henseleit solution was gassed continuously with 95% O2/5% CO2 to maintain a pH of 7.4.35 Real-time FRET experiments were performed at room temperature. Images were captured every 5 s using the × 40 oil-immersion objective of an inverted microscope (Nikon, Champigny sur Marne, France) connected to a Cool SNAP HQ2 camera (Photometrics, Tucson, AZ, USA) controlled by the Metafluor software (Molecular Devices, Sunnyvale, CA, USA). The donor (mTurquoise2)36 was excited during 300 ms by a xenon lamp (Nikon) using a 440/20BP filter and a 455LP dichroic mirror. Dual-emission imaging of donor and acceptor was performed using a dual-view emission splitter equipped with a 510 LP dichroic mirror and BP filters 480/30 and 535/25 nm, respectively.
Results are expressed as mean±S.E.M. The Origin software (Northampton, MA, USA) was used for statistical analysis. Differences between groups have been analyzed by one-way ANOVA and Student's t-test. A value of P<0.05 were considered as statistically significant. The number of animals, cells and independent experiments performed is indicated in the figure legends.
This work has been funded by INSERM (AMG, CB, GV, RF), the Investment for the Future program ANR-11-IDEX-0003-01 within the LABEX ANR-10-LABX-0033 (CB, GV, RF) and ANR (ANR-13-ISV1-0001-01, CB). ZW and DL are supported by a fellowship from the China Scholarship Council. The Leica microscope was funded by CORDDIM (Investissement 2010, COD100296). We thank Florence Lefebvre for her assistance in adult cardiomyocyte isolation and Valérie Domergue for animal housing and care at the Animex facility, IPSIT, Châtenay-Malabry, France. We also thank Christine Longin for Transmission Electron Microscopy at INRA, Jouy-en-Josas, France. We are also thankful to Jessica Sabourin, Cécile Martel, Delphine Mika and Jerôme Leroy for helpful discussions.
adenine nucleotide translocase
carbonyl cyanide m-chlorophenyl hydrazone
mitochondrial membrane potential
exchange protein directly activated by cAMP
mitochondrial calcium uniporter
mitochondrial Na+/Ca2+ exchanger
mitochondrial permeability transition
protein kinase A
reactive oxygen species
soluble adenylyl cyclase
full-length soluble adenylyl cyclase
truncated soluble adenylyl cyclase
About this article
Supplementary Information accompanies this paper on Cell Death and Disease website (http://www.nature.com/cddis)
Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018
Cell Death & Differentiation (2018)