BTG3 (B-cell translocation gene 3) is a p53 target that also binds and inhibits E2F1. Although it connects two major growth-regulatory pathways functionally and is downregulated in human cancers, whether and how BTG3 acts as a tumor suppressor remain largely uncharacterized. Here we present evidence that BTG3 binds and suppresses AKT, a kinase frequently deregulated in cancers. BTG3 ablation results in increased AKT activity that phosphorylates and inhibits glycogen synthase kinase 3β. Consequently, we also observed elevated β-catenin/T-cell factor activity, upregulation of mesenchymal markers, and enhanced cell migration. Consistent with these findings, BTG3 overexpression suppressed tumor growth in mouse xenografts, and was associated with diminished AKT phosphorylation and reduced β-catenin in tissue specimens. Significantly, a short BTG3-derived peptide was identified, which recapitulates these effects in vitro and in cells. Thus, our study provides mechanistic insights into a previously unreported AKT inhibitory pathway downstream of p53. The identification of an AKT inhibitory peptide also unveils a new avenue for cancer therapeutics development.
BTG3 is a member of the B-cell translocation gene/transducer of ErbB2 (BTG/Tob (transducer of ERBB2)) antiproliferative protein family that also includes BTG1, BTG2/PC3/Tis21 (TPA-induced sequence 21), BTG4, Tob1, and Tob2.1 The members of this protein family are characterized by a conserved N-terminal domain containing box A and box B signature motifs, and a variable C-terminal domain.2 Overexpression of BTG/Tob proteins is associated with inhibition of cell cycle progression, which is mostly mediated by their conserved N-terminal domain. For example, BTG2 inhibits G1-to-S progression via the downregulation of cyclin D1 and cyclin E,3 whereas BTG3 binds and inhibits E2F1, a transcription factor important for S-phase entry.4 Both BTG2 and BTG3 are transcriptional targets of the tumor suppressor p53, thus linking this family of proteins with stress response.4, 5 In addition, the N-terminal conserved domains of the BTG/Tob proteins are also known to interact with CAF1 (CCR4-associated factor 1), thereby modulating mRNA deadenylation6, 7 or cell proliferation.8 Less is known regarding the functions of the structurally diverse C terminus. The C termini of BTG1 and BTG2 interact with the protein arginine methyltransferase PRMT1 (protein arginine methyltransferase 1).9 More recently, the C terminus of BTG3 was found to bind CHK1 (checkpoint kinase 1) and safeguard genomic stability.10 Despite these findings, the biological relevance of this domain remains mostly uncharacterized. Downregulation of BTG3 was found in human cancers,11, 12, 13, 14 implicating a possible role as a tumor suppressor.
In contrast to the BTG/Tob proteins, the Ser/Thr kinase AKT acts as a prosurvival and proproliferation factor. AKT is involved in the regulation of many cellular processes via various downstream effectors such as mammalian target of the rapamycin (mTOR) in protein synthesis15 and the transcription factors nuclear factor- κB and FOXO (forkhead box O) in cell survival.16, 17 Importantly, it allows the stabilization and subsequent nuclear localization of β-catenin by phosphorylating and inhibiting glycogen synthase kinase 3β (GSK3β), thus connecting β-catenin with cell growth and migration.18, 19 The activation of AKT requires the phosphorylation of its Thr308 and Ser473 residues by PDK1 (phosphoinositide-dependent kinase-1) and mTORC2 (mammalian target of rapamycin complex 2), respectively,20, 21 which is mediated by the binding of the N-terminal pleckstrin homology (PH) domain to membrane phosphatidylinositol-3,4,5-trisphosphate. Upstream stimuli such as growth factors activate receptor tyrosine kinase, which triggers the activation of phosphoinositide 3-kinase (PI3K) and leads to the generation of PIP3. The tumor suppressor PTEN (phosphatase and tensin homolog) converts PIP3 to phosphatidylinositol-4,5-bisphosphate, thus antagonizing PI3K-AKT signaling.22
The epithelial-to-mesenchymal transition (EMT) in tumors is characterized by a decrease in cell–cell adhesion and an increase in cell invasion and motility, and is believed to contribute to metastasis.23, 24 Many signaling events have been shown to be involved: among them, Wnt signaling inhibits GSK3β-mediated phosphorylation and degradation of β-catenin. The increased β-catenin translocates to the nucleus, where it induces the expression of genes encoding proteins such as vimentin and fibronectin that specify EMT.25
We demonstrated previously that BTG3 binds and inhibits E2F1, and therefore preventing S-phase entry and also maintaining G2/M arrest.4 We also showed that in normal human fibroblasts, BTG3 functions as a fail-safe anticancer barrier.14 Regardless of these findings, several issues remain unaddressed. For example, despite the enhanced E2F1 activity and its link with the induction of apoptosis, cell death was never observed in BTG3-depleted cells. Here we delineated further the effects of the loss of BTG3 and demonstrated its role in safeguarding the AKT-GSK3β pathway, and, by crosstalk with the Wnt/β-catenin pathway, its role as a barrier to tumor progression.
BTG3 interacts with and inhibits AKT
The AKT signaling pathway controls cell proliferation, growth, and survival, and is frequently deregulated in cancers as a result of gene mutation, amplification, or loss or mutation of its negative regulator PTEN.26 As BTG3 has been implicated in tumor suppression,14, 27 we wondered whether it would be involved in the regulation of AKT. To determine whether BTG3 has an impact on AKT signaling, we first performed overexpression or knockdown experiments in 293 T cells, using AKT phosphorylation as an indicator. As shown in Figure 1a, overexpression of BTG3 suppressed AKT Thr308 phosphorylation (pT308), whereas its downregulation using a specific short hairpin (sh) RNA had the opposite effect. Similarly, overexpression of BTG3 in the prostate cancer cell line DU145 dampened serum-induced AKT activation (Figure 1b), suggesting that this is not a cell-type-specific effect. In vitro, the C-terminal domain (amino acids 108–252) of recombinant BTG3 interacted with AKT through the N-terminal and central regions that host the PH and kinase domains (Figures 1c and d). Further analysis of the BTG3 C terminus identified the region between amino acids 147 and 252 that mediates this interaction (Supplementary Figure S1). The interaction between endogenous BTG3 and AKT in 293 T cells was also detected by co-immunoprecipitation using the anti-BTG3 antibody (Figure 1e), verified by reciprocal immunoprecipitation using the anti-AKT antibody (Figure 1f). Although full-length BTG3 significantly inhibited AKT activation, the BTG3-d4 mutant (amino acids 147–252 deleted) that lacks the AKT interaction domain could not (Figure 1g and Supplementary Figure S1), suggesting that direct interaction is required for the inhibition.
BTG3 impedes AKT recruitment to the plasma membrane
AKT is phosphorylated and activated at the plasma membrane by its upstream kinases. In an attempt to determine the underlying mechanism for BTG3-mediated inhibition of AKT, we examined the level of membrane-associated AKT in the presence or absence of ectopically expressed BTG3. Upon serum stimulation, AKT at the plasma membrane was increased in control vector-transfected 293 T cells. Such an increase was abolished in cells with ectopically expressed BTG3 (Figure 2a). Furthermore, serum stimulation enhanced the interaction of AKT with its upstream kinase PDK1, and such interaction was disrupted by ectopically expressed BTG3 (Figures 2b and c).
To provide further support for the idea that BTG3 mediates the suppression of AKT membrane localization, we then used a fusion construct that expressed green fluorescent protein (GFP) fused to the PH domain of AKT (GFP-PH-AKT).28 In 293 T cells, which express high PI3K activity, GFP-PH-AKT resided mostly in the plasma membrane (Figure 2d). Upon coexpression of the full-length BTG3, the membrane localization of GFP-PH-AKT was partially disrupted (Figures 2d and e). Such interference was not observed when the AKT-interaction-defective mutant d4 was used (Figures 2d and f), indicating that the suppression was most likely mediated through direct interaction. As a control, BTG3 did not significantly impact the membrane localization of the GFP-fused PH domain of Grp1 (general receptor of phosphoinositides 1) (GFP-PH-Grp1) (Supplementary Figure S2), suggesting that the regulation is specific to PH-AKT. Collectively, our data suggest that BTG3 inhibits the activation of AKT, at least in part, by preventing AKT from binding to the plasma membrane.
BTG3 suppresses AKT-GSK3β-β-catenin signaling
To understand further the functional impact of BTG3-AKT interaction, events that lie downstream of AKT activation such as AKT-GSK3β signaling were investigated. Concordant with AKT inhibition (Figure 1b), overexpression of BTG3 in U2OS cells led to decreased GSK3β phosphorylation. This enhanced GSK3β activity culminated in reduced levels of β-catenin, likely as a result of enhanced degradation29 (Figure 3a). Conversely, after depletion of BTG3, the AKT and GSK3β phosphorylation levels were enhanced (Figure 3b) and the amount of nuclear β-catenin increased, as demonstrated by cell fractionation (Figure 3c). The increased nuclear presence of β-catenin after BTG3 depletion also markedly enhanced the activity of the β-catenin/T-cell factor (TCF) reporter (Figure 3d) as well as the production of its endogenous downstream targets, fibronectin, and vimentin (Figure 3e). Levels of Slug, ZEB1 (zinc-finger E-box-binding homeobox 1), and N-cadherin were also increased. As fibronectin, vimentin, and the other molecules are markers of EMT,25 cell migration was assessed using a transwell assay. A 50% increase in cell migration was observed after downregulation of BTG3 by inducible shRNA (Figure 3f). Of note, levels of E-cadherin were not significantly changed by BTG3 depletion in U2OS cells (data not shown). The inhibitory effects of BTG3 on AKT signaling, fibronectin and ZEB1 expression, the β-catenin/TCF reporter, and cell migration were also observed in PC3 cells overexpressing BTG3 (Supplementary Figure S3).
To further test whether BTG3-dependent inhibition of β-catenin was mediated through GSK3β, the GSK3-specific inhibitor CT99021 was used. As predicted, overexpression of BTG3 suppressed the activity of β-catenin/TCF, and such inhibition was abolished in the presence of CT99021 (Figure 3g), thus supporting the role of GSK3β in mediating the inhibitory effect of BTG3. Taken together, these results implicate BTG3 in the negative regulation of the AKT-GSK3β-β-catenin signaling axis.
A BTG3-derived peptide recapitulates the activity of the full-length BTG3 protein in suppressing AKT
To understand further the molecular basis of AKT interaction and suppression, five peptides (C1–C5) spanning the BTG3 C-terminal AKT interaction domain (amino acids 147–252) were synthesized (Figures 4a and b). These peptides were first tested for their ability to compete with the full-length BTG3 for binding AKT in GST pull-down assays. Among the five peptides tested, only C5 was able to compete and interfere with the interaction between BTG3 and AKT (Figure 4c). Consistently, transfected C5 but not C1 or the control unrelated influenza hemagglutinin (HA) peptide suppressed the phosphorylation of AKT and GSK3β in 293 T cells (Figure 4d), indicating that the C5 peptide might functionally mimic the full-length BTG3 protein. In support of this idea, the levels and the activity of nuclear β-catenin were reduced in the C5 but not in the C1 peptide-transfected cells (Figures 4e and f). As a control, neither the C5 nor the C1 peptide had any detectable effect on extracellular signal-regulated protein kinase (ERK) phosphorylation (Figure 4g), thus demonstrating the specificity of the regulation. These effects were reproduced with a C5-derived peptide spanning the C-terminal (C5-C) but not the N-terminal half (C5-N) of C5 (Supplementary Figure S4), thus revealing a minimal AKT-interacting motif in the region between amino acids 241 and 252.
Identification of residues in the BTG3 C-terminal peptide critical for inhibition of AKT
Based on the study by Wu et al.30 in which a small-molecule AKT inhibitor with aromatic ring structures was shown to lock the PH and the kinase domain of AKT in an inactive conformation, we speculated that a residue or residues with similar structure(s) in C5-C might act similarly. By aligning the regional amino-acid sequences of BTG3 from different species, we found that H242 and W243 were conserved in mammals and amphibians. The other two ring-type residues, H247 and H252, although conserved among mammals, were not present in frog BTG3 (Figure 5a). We therefore conducted alanine substitution at four residues: H242, W243, H247, and H252 (Figure 5b). Interestingly, mutation at H242 and W243 abolished the inhibitory activity of C5-C, whereas mutation at H247 and H252 had no apparent effect on BTG3-AKT interaction (Figure 5c), AKT signaling (Figure 5d), and the β-catenin/TCF reporter activity (Figure 5e). An in silico model fitting was then attempted. The deduced model indicated that, similar to the AKT inhibitor, residues H242 and W243, when docked to the AKT1 PH kinase interdomain region (Protein Data Bank, PDB code 3O96) in the context of the C5 peptide, were able to make close contacts with functional groups stemming from the PH and the kinase domains (Figure 5f), suggesting a similar mode of AKT1 inactivation.
To verify the importance of residues H242 and W243 in the context of the full-length protein, we then generated a mutant BTG3 with these two residues mutated to A. Similar to the mutant peptide, the BTG3 AA mutant (BTG3-mHW) was less efficient in suppressing AKT phosphorylation when compared with the wild-type protein (Figure 5g). Our study thus identified at least two residues in the C terminus of BTG3 that mediate the inhibition of AKT.
BTG3 suppresses cell growth in three-dimensional culture
Three-dimensional (3D) matrix culture has been shown to mimic more closely the stromal microenvironment in vivo than 2D culture, and to allow phenotypic discrimination between malignant and nonmalignant cells.31, 32 As a step toward understanding the role of BTG3 in tumor progression, we first determined its impact on cells grown in a 3D matrix. For this assay, we chose to use PC3 cells that lack PTEN and have high AKT activity. Thus, we established PC3 Tet-On cell lines (ovBTG3) that inducibly express an exogenous myc-tagged BTG3 upon the addition of doxycycline (Figure 6a). Consistent with our observations in other cells, AKT phosphorylation in these cells was markedly reduced (Figure 6a). A significant reduction in AKT phosphorylation in ovBTG3 cells was also observed in the absence of doxycycline, likely because of leaky expression. The growth rates of the TR (control cells that express only the tetracycline regulator) and ovBTG3 cells in a 3D matrix (Matrigel) were compared. As previously described for PC3 cells,33 PC3-TR cells grew in spheroids on Matrigel, underwent polarized differentiation, and formed acinar structures. By contrast, the PC3-ovBTG3 cells formed mostly irregular spheroids and incompletely developed lumens (Figures 6b and c), suggesting a disrupted polarization. Furthermore, the plating efficiency was significantly reduced with the ovBTG3 cells compared with the control PC3-TR cells (Figure 6d). However, despite these differences, both cell lines formed invasive stellates upon prolonged incubation (more than 2 weeks, data not shown), suggesting that, at least in PC3 cells, although BTG3 overexpression alone is capable of suppressing AKT, it is insufficient in inhibiting invasive growth in 3D culture. Similar results were obtained with another PC3-ovBTG3 clone (Supplementary Figure S5), thus excluding possible clonal effects. Collectively, these results suggest that BTG3 not only dampens cell colonization but also appears to hamper the polarization of PC3 cells in the 3D matrix.
To link the above observation with the regulation of AKT, we then compared in 3D culture the activity of BTG3 WT and the BTG3-mHW mutant impaired in AKT inhibition. Our data showed that unlike the PC3-ovBTG3 WT, two of the PC3-ovBTG3-mHW (nos. 2 and 4) clones grew in regular spheroids as the control PC3-TR cells (Figures 6e and g), despite comparable levels of BTG3 were expressed (Figure 6f). This result thus demonstrated a functional relevance of the residues H242 and W243 in growth in addition to their involvement in the regulation of AKT phosphorylation and activation.
BTG3 suppresses tumor growth in a xenograft mouse model
To determine the role of BTG3 in tumor suppression in a more physiological setting, we performed xenograft studies by implanting the PC3-TR (control) or PC3-ovBTG3 cells subcutaneously into immunosuppressed nude mice. The tumors produced were compared for volume and histology. As shown in Figure 7a, tumors produced by PC3-ovBTG3 cells were smaller than those from the PC3-TR control cells. Analysis of lysates prepared from the tumors showed that they retained the ectopically expressed BTG3 (Figure 7b). Histologically, some of the PC3-ovBTG3 tumors displayed a large area of necrosis, mostly at the core and some at the periphery of the tumor, which might account for their smaller size (Figure 7c, upper panel). Immunohistochemical staining also confirmed reduced phospho-AKT (pS473) (Figure 7d) and β-catenin levels (Figure 7e) in PC3-ovBTG3 tumors, thus providing further support to our cell-based studies. These observations were reproduced with another PC3-ovBTG3 clone in an independent experiment (Supplementary Figure S6), thus excluding possible clonal effects.
Increased AKT phosphorylation in human prostate cancer specimens with reduced BTG3 expression
To determine the clinical relevance of the BTG3-AKT axis, we performed IHC staining on paraffin-embedded sections of clinical prostate cancer specimens to compare the levels of BTG3 and phospho-AKT (pT308) (Figure 7f). Of the 188 tumor tissue sections (from 94 cases) and 20 normal/adjacent normal tissue sections (from 20 cases) examined, significant reduction in BTG3 staining was found in tumor tissues compared with normal tissues (P=0.03) (Figure 7g). This is in agreement with our previous observation and with observations from others that BTG3 is downregulated in prostate cancers.13, 14 Within the BTG3-downregulated tumors (≦Normal; Figure 7h), it was observed that phospho-AKT (pT308) staining was increased as the disease progresses from differentiated or poorly differentiated (grades 1–3) to undifferentiated (grade 4) stage (P=0.02; Figure 7h). However, this trend was not as apparent in tumors expressing higher level of BTG3 (>Normal; Figure 7h), suggesting the existence of other AKT regulator in these tumors. These results highlight an inverse relationship between the levels of BTG3 and AKT activity with respect to disease progression, further supporting a likely involvement of BTG3-AKT regulation in prostate cancer.
Deregulation of the PI3K-AKT pathway is known to have a prominent role in various cancers, especially in the prostate.34 Here we demonstrated that BTG3 guards against the AKT-GSK3β-β-catenin signaling axis by binding and preventing AKT from localizing to the plasma membrane.
BTG3 as a suppressor of tumorigenesis
The BTG protein family members are known to be antiproliferative; however, their role in tumor suppression has not been examined extensively. Loss of expression including loss of heterozygosity or promoter methylation of the BTG family genes have been reported in different types of cancers.11, 12, 13, 35, 36, 37 Knockout mouse models for BTG3 and Tob were established,27, 38 which either succumbed to lung tumors at an old age in the case of Btg3 knockouts27 or spontaneously developed a variety of tumors in the case of Tob knockouts.38 Although increased cyclin D1 expression was implicated in tumor formation in the Tob-null mice, it was not clear why Btg3 deficiency caused lung tumors in mice. Data presented in this report together with our recent study14 provide evidence that BTG3 acts to regulate at least two proproliferation pathways frequently deregulated in cancers: the MAPK (mitogen-activated protein kinase) and the AKT pathways. Thus, overexpression of BTG3 inhibited AKT signaling, and downregulation of BTG3 increased the levels of phospho-ERK and phospho-AKT. In normal cells, this initial response was inactivated soon after the engagement of the senescence program, whereas in genetically predisposed cells malignant transformation might be triggered, and we also showed that increased AKT signaling led to the inhibition of GSK3β and activation of β-catenin.
Although our PC3 xenograft study showed that BTG3 could be a tumor suppressor, a reciprocal knockdown in U2OS cells failed to promote xenograft tumors (data not shown). One reason that could account for this outcome might reside in the U2OS cells, which by themselves could not form tumors in mice (data not shown). Although BTG3 downregulation was shown to promote expression of EMT markers and cell migration in these cells, the effects appeared insufficient to drive tumor formation in vivo, at least for U2OS cells. One could interpret these negative results as meaning that the loss of BTG3 alone is not sufficient to drive tumorigenesis. This interpretation is supported by our recent finding that the loss of BTG3 could trigger senescence in normal cells14 and also by the findings of others that mice with transgenic AKT expression developed prostate intraepithelial neoplasia but not cancers.39 Thus, one could predict that additional events are required to trigger cancerous progression. To address its tumor suppressor role, a Btg3 conditional allele that allows inducible ablation of Btg3 in different cancer models may provide more physiologically relevant answers.
BTG3 and PTEN in the defense against tumorigenesis
The data presented here and our recently published study regarding the role of BTG3 in genomic stability10 underscore the surprising functional similarities between BTG3 and PTEN. Both are known to be transactivated by p53 upon genotoxic stress,4, 40 and inactivation of either one impairs the checkpoint response.4, 41 Furthermore, both negatively regulate PI3K/AKT signaling, although different mechanisms are used in each case. This mechanistic difference may explain, at least in part, the differential impact on prostate cancer when they are lost. PTEN deficiency occurs in 60–80% of prostate cancers42 with a clear causal relationship. The impact of reduced BTG3 expression appears to be less pronounced. In addition, unlike Pten-null mice that died in utero,43 the Btg3-knockout mice developed normally and were not particularly tumor-prone until 21 months, at which time lung tumors were detected.27 The possibility that the Btg3 deficiency was complemented by the other members of the gene family such as Btg2 has yet to be excluded.
Another striking similarity between BTG3 and PTEN is their impact on CHK1. Loss of PTEN impairs CHK1 activity via AKT-dependent phosphorylation and its cytoplasmic sequestration.41 On the other hand, BTG3 safeguards CHK1 activation by promoting its K63-linked ubiquitination and chromatin association.10 Thus, through different pathways, both proteins appear to regulate the activity of CHK1. Additionally, as demonstrated here, BTG3 could indirectly promote CHK1 activity by inhibiting AKT activation.
Development of a potential AKT inhibitor
Various inhibitors targeting the PI3K pathway have been developed; many of them are ATP (adenosine triphosphate)-competitive.44 The BTG3-derived, AKT inhibitory small peptide we identified here represents a distinct category of AKT inhibitors. As with some of the existing AKT inhibitors, the BTG3 peptide acts allosterically, most likely by keeping the kinase in a ‘PH-in’conformation45 as suggested by our data here. However, unlike those small-molecule AKT inhibitors, the small BTG3 peptide is based on a cellular protein and thus might have the advantage of lower cytotoxicity and higher specificity. By combining with a proper delivery system, a modified BTG3 peptide or its derivatives with improved stability could be developed. In light of the important role of AKT in tumorigenesis, angiogenesis, and metastasis, the BTG3 peptide or its derivatives may serve as promising leads for cancer drug development.
Materials and Methods
To establish inducible osteosarcoma U2OS and prostate cancer PC3 Tet-On stable cell lines for BTG3 knockdown or overexpression, U2OS-TR cells (tetracycline regulator-expressing)46 or PC3-TR cells were stably transfected with pBabe-H1-shBTG34 or pcDNA4-TO-BTG3, respectively. The U2OS BTG-knockdown stable cell line was maintained regularly in complete DMEM containing 2.5 μg/ml blasticidin (InvivoGen, San Diego, CA, USA) and 0.8 μg/ml puromycin (Sigma-Aldrich, St. Louis, MO, USA), and the PC3 BTG3-overexpressed line in F-12 K plus 2.5 μg/ml blasticidin and 100 μg/ml Zeocin (InvivoGen). Induction was performed using 1 μg/ml doxycycline (Sigma-Aldrich).
The human embryonic kidney 293T cells and the prostate cancer cell line DU145 were maintained, respectively, in DMEM and RPMI containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (all from Gibco, Life Technologies, Grand Island, NY, USA).
Plasmids and constructs
To generate GST-tagged N- and C-terminal BTG3 truncation constructs, the corresponding regions of BTG3 were amplified by PCR and cloned into the BamHI and XhoI sites of the pGEX4T-1 vector (Amersham Biosciences, Piscataway, NJ, USA). The plasmids expressing GST-tagged AKT full-length and truncation mutants were generated by PCR amplification of the corresponding coding regions from pcDNA3-myr-HA-AKT (kindly provided by Jeffrey J-Y Yen, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan) and cloned between the EcoRI and XhoI sites of pGEX4T-1. For mammalian expression of HA-AKT, the full-length AKT was amplified by PCR from pcDNA3-myr-HA-AKT and cloned between the EcoRI and XhoI sites of the pcDNA3-HA vector. The myristoylation (myr) sequence was removed to allow dynamic regulation of AKT in cells. All sequences amplified by PCR were verified by DNA sequencing.
GST pull-down assay
The assay was performed essentially as described previously.46 For peptide competition, 20 × molar excess of the peptide was included in the reaction to compete with the full-length BTG3 protein.
Plasmid and siRNA transfection
Plasmids were transfected using Lipofectamine 2000 (Invitrogen Life Technologies, Carlsbad, CA, USA) except in 293 T cells, for which the calcium phosphate precipitation method was used. All siRNAs were synthesized by Sigma-Aldrich. Transfection of siRNA was performed using Oligofectamine (Invitrogen Life Technologies). The sequences targeted by the BTG3 siRNAs were 5′-IndexTermGGCTAGTTCGAAAACATGA-3′ (BTG3-1) and 5′-IndexTermTTGAGAGGTTTGCTGAGAA-3′ (BTG3-2).
All peptides were commercially synthesized at over 70% purity (Kelowna International Scientific Inc., New Taipei, Taiwan). Cells were plated 1 day before transfection at a density that would reach ~50% confluence at the time of transfection. Peptides (0.5–1 μg/35 mm dish) were transfected using the TransPass P Transfection Reagent (New England BioLabs, Beverly, MA, USA) as directed.
Cell lysis and immunoblotting
Cell lysates were prepared as described.47 Immunoblotting was carried out using the following antibodies against: actin (A2066), α-tubulin (T6074), and fibronectin (F6140) from Sigma-Aldrich; GST (sc-138), His (sc-803), and Myc (sc-40) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); HA (Covance, Berkeley, CA, USA); β-catenin (no. 610154) and protein kinase Bα/AKT1 (no. 610860) from BD Biosciences (San Jose, CA, USA); phospho-AKT (Thr308) (no. 4056), phospho-AKT (Ser473) XP (no. 4060), phospho-GSK-3β (Ser 9) (no. 9323), GSK-3β (no. 9315), vimentin (no. 3932), N-cadherin (no. 4061), Slug (no. 9585), and ZEB1 (no. 3396) from Cell Signaling Technologies (Danvers, MA, USA); and ERK1 and 2 (pTpY185/187) (no. 44680 G) and ERK1 and 2 (no. 44-654 G) from Invitrogen Life Technologies. The rabbit anti-BTG3 antibody has been described previously.4
Lysis of cells and immunoprecipitation were carried out as described previously10 using anti-BTG3 or anti-AKT (no. 610860; BD Biosciences).
Luciferase reporter assay
U2OS cells were first transfected with control or BTG3 siRNA using Oligofectamine (Invitrogen Life Technologies). The following day, cells were transfected with the β-catenin/TCF reporter 8 × FOPFlash (mutant) or 8 × TOPFlash (wild-type) (Addgene, Cambridge, MA, USA). Assays were performed as described previously.4
Transwell migration assay
Cell migration was assayed in a 24-well Boyden chamber (Corning Costar, Corning, NY, USA). Equal numbers of Tet-On U2OS stable cells induced or not induced to express BTG3 shRNA were plated in serum-free medium in the upper compartment of the Boyden chamber. The lower compartment contained 700 μl of 10% FBS in the medium, which was used as a chemoattractant. Cells that migrated through the insert after 16–18 h were fixed with 100% methanol and stained with Wright–Giemsa stain. Cells on the lower surface of each insert, in a total of five random objective fields, were counted using a light microscope at × 200 magnification. Student’s t-test was applied to assess the significance of differences between mean counts.
PC3 cells were trypsinized and resuspended in F12K medium (Invitrogen Life Technologies) containing 10% FBS, 1% Pen/Strep mix, and 2% Matrigel (BD Biosciences) to a final concentration of 5000 cells/ml. Two thousand cells were seeded into each well of an 8-well chamber slide (Millipore EZ; Millipore Corp., Bedford, MA, USA) precoated with 60 μl of Matrigel. The culture was refreshed with 400 μl medium every 4 days until the day of the assay. Irregular spheroids are judged based on the staining of 4',6-diamidino-2-phenylindole or laminin β1. Those that are not as rounded as the TR control and with short buds or sprouts are determined to be irregular. Plating efficiency was determined by the number of spheroids formed at the indicated time point divided by the number of cells plated on Matrigel. For protein analysis, spheroids in 3D culture were harvested and extracted as described.31
For membrane localization, 293 T cells were transfected with plasmids expressing either GFP or GFP-PH-AKT,28 together with either full-length BTG3 or the d4 mutant. Localization was analyzed by confocal microscopy and quantified using ZEN 2008 software (Carl Zeiss, Oberkochen, Germany).
For staining of the 3D culture, the spheroids were fixed in chamber slides with 4% paraformaldehyde for 30 min at room temperature before permeabilization. The anti-laminin β1 antibody (ab69633; Abcam, Cambridge, MA, USA) was used at 1 : 200 dilution. Images were captured using a Zeiss LSM510 confocal microscopy system (Carl Zeiss Microscopy GmbH, Jena, Germany).
Generation of docking model
To derive the model, we first positioned H242 and W243 (blue sticks) of the peptide to the binding region of the imidazoquinoxaline and benzylpiperidine groups of the AKT inhibitor VIII (PDB code 3O96; Wu et al.30). This was followed by molecular dynamic simulation (100 ps) and energy minimization to relax the peptide and side-chain atoms of the protein at the binding site. The docking and simulation were carried out using Discovery Studio (Accelrys Inc., San Diego, CA, USA). Figure 5e was generated using PyMol (DeLano Scientific LLC, Palo Alto, CA, USA).
Mouse xenograft studies
All animal studies were conducted according to the protocol approved by the Institutional Animal Care and Utilization Committee of Academia Sinica (AS IACUC, Protocol ID 11-12-271). Athymic (Bltw:NU-Foxn1nu) male mice (BioLASCO, Taipei, Taiwan) were maintained in pathogen-free conditions. Stable clones of TR- and BTG3-overexpressing PC3 cells (ovBTG3) (2 × 106) were injected subcutaneously in 0.1 ml of Matrigel (BD Biosciences) into the backs of 4-week-old mice. Animals received doxycycline (200 μg/ml) in the drinking water supplemented with 1% sucrose after the injection. The solution was refreshed two times a week and throughout the duration of the experiment. Tumor sizes were measured every week and the tumor volume was calculated using the formula V=0.5 × a × b2, where a is the length and b is the width (in mm).
Human prostate cancer tissue microarrays (PR2085B; US Biomax Inc., Rockville, MD, USA) were stained as described previously,14 except that the slides were treated with 3% H2O2 before antigen retrieval. The antibodies used were anti-BTG3 (1 : 150; 4) and anti-AKT pT308 (1 : 250; sc-16646-R; Santa Cruz Biotechnology, Dallas, TX, USA). Images were taken using a Zeiss Imager A1 microscope (Carl Zeiss Microscopy GmbH). H-scores yielded from normal and tumor sections were then analyzed using Student’s t-test.
For IHC staining of xenograft tumors, anti-phospho-AKT Ser473 (1 : 100; no. 3787; Cell Signaling Technologies) and anti-β-catenin antibodies (1 : 100; no. 610154; BD Biosciences) were used.
B-cell translocation gene
checkpoint kinase 1
extracellular signal-regulated protein kinase
green fluorescent protein
glycogen synthase kinase 3β
general receptor of phosphoinositides 1
mammalian target of the rapamycin
phosphatase and tensin homolog
zinc-finger E-box-binding homeobox 1
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We are grateful to Dr. Rieko Ohki for providing the GFP-PH constructs, and the Pathology Core at IBMS, Academia Sinica for assistance with IHC. This work was supported by grants from Ministry of Science and Technology of Taiwan and Academia Sinica (to S-Y Shieh).
The authors declare no conflict of interest.
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Cheng, YC., Chen, PH., Chiang, HY. et al. Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT. Cell Death Dis 6, e1584 (2015). https://doi.org/10.1038/cddis.2014.550
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