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Post-Transplant Events

Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 45, pages 1638–1644 (2010)Cite this article

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Abstract

The hair follicles of recipients of allogeneic hematopoietic SCT (HSCT) constitute the tissue with the greatest need for regeneration after high-dose chemotherapy. Previous studies have shown a lack of donor-derived DNA in the hair follicles of recipients. Therefore, we carried out a study to determine whether male donor-derived genetic material can be found in female recpients’ hair follicles after HSCT. Fluorescent-based PCR with analyses of Y-chromosome STR (Y-STR) and RQ-PCR with the sex-determining region Y (SRY) were used independently to evaluate chimerism status. Our results proved the existence of donor-derived stem DNA in the recipients’ hair follicle cells. This report undermines the validity of data indicating that hair follicle cells maintain 100% of recipient origin.

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References

  1. Abkowitz JL . Can human hematopoietic stem cells become skin, gut, or liver cells? [Letter]. N Engl J Med 2002; 346: 770–772.

    Article  Google Scholar 

  2. Körbling M, Katz RL, Khanna A, Ruifrok AC, Rondon G, Albitar M et al. Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells. N Engl J Med 2002; 346: 738–746.

    Article  Google Scholar 

  3. Suratt BT, Cool CD, Serls AE, Chen L, Varella-Garcia M, Shpall EJ et al. Human pulmonary chimerism after hematopoietic stem cell transplantation. Am J Respir Crit Care Med 2003; 168: 318–322.

    Article  Google Scholar 

  4. Dauber EM, Dorner G, Mitterbauer M, Wenda S, Faé I, Glock B et al. Discrepant results of samples taken from different tissues of a single individual. Int Congr Series 2004; 1261: 48–49.

    Article  CAS  Google Scholar 

  5. Rovó A, Gratwohl A . Plasticity after allogeneic hematopoietic stem cell transplantation. Biol Chem 2008; 389: 825–836.

    Article  Google Scholar 

  6. Andréasson H, Nilsson M, Budowle B, Lundberg H, Allen M . Nuclear and mitochondrial DNA quantification of various forensic materials. Forensic Sci Int 2006; 164: 56–64.

    Article  Google Scholar 

  7. Bond JW, Phil D . Value of DNA evidence in detecting crime. J Forensic Sci 2007; 52: 128–136.

    Article  CAS  Google Scholar 

  8. Khan F, Agarwal A, Agrawal S . Significance of chimerism in hematopoietic stem cell transplantation: new variations on an old theme. Bone Marrow Transplant 2004; 34: 1–12.

    Article  CAS  Google Scholar 

  9. McCann SR, Crampe M, Molloy K, Lawler M . Hemopoietic chimerism following stem cell transplantation. Transfus Apher Sci 2005; 32: 55–61.

    Article  Google Scholar 

  10. Hong YC, Liu HM, Chen PS, Chen YJ, Lyou JY, Hu HY et al. Hair follicle: a reliable source of recipient origin after allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant 2007; 40: 871–874.

    Article  CAS  Google Scholar 

  11. Thiede C, Prange-Krex G, Freiberg-Richter J, Bornhäuser M, Ehninger G . Buccal swabs but not mouthwash samples can be used to obtain pretransplant DNA fingerprints from recipients of allogeneic bone marrow transplants [letter]. Bone Marrow Transplant 2000; 25: 575–577.

    Article  CAS  Google Scholar 

  12. Tran SD, Pillemer SR, Dutra A, Barrett AJ, Brownstein MJ, Key S et al. Differentiation of human bone marrow-derived cells into buccal epithelial cells in vivo: a molecular analytical study. Lancet 2003; 361: 1084–1088.

    Article  Google Scholar 

  13. Allouche M, Hamdoum M, Mangin P, Castella V . Genetic identification of decomposed cadavers using nails as DNA source. Forensic Sci Int Genet 2008; 3: 46–49.

    Article  CAS  Google Scholar 

  14. Di Martino D, Giuffrè G, Staiti N, Simone A, Todaro P, Saravo L . Laser microdissection and DNA typing of cells from single hair follicles. Forensic Sci Int 2004; 146 (Suppl 1): S155–S157.

    Article  CAS  Google Scholar 

  15. Imanishi D, Miyazaki Y, Yamasaki R, Sawayama Y, Taguchi J, Tsushima H et al. Donor-derived DNA in fingernails among recipients of allogeneic hematopoietic stem-cell transplants. Blood 2007; 110: 2231–2234.

    Article  CAS  Google Scholar 

  16. Pearce L, Lim ZY, Usai M, Ho AY, Mufti GJ, Pagliuca A . Mixed donor chimaerism in recipient fingernails following reduced-intensity conditioning haematopoietic SCT [letter]. Bone Marrow Transplant 2008; 42: 361–362.

    Article  CAS  Google Scholar 

  17. Rovó A, Meyer-Monard S, Heim D, Arber C, Passweg JR, Gratwohl A et al. No evidence of plasticity in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation. Exp Hematol 2005; 33: 909–911.

    Article  Google Scholar 

  18. Gratwohl A . Principles of conditioning. In: Apperley J, Carreras E, Gluckman E, Gratwohl A, Masszi T (eds). Haematopoietic Stem Cell Transplantation. The EBMT Handbook, 5th edn. Forum Service Editore: Genoa, Italy, 2008, pp 129–142.

    Google Scholar 

  19. Nollet F, Billiet J, Selleslag D, Criel A . Standardisation of multiplex fluorescent short tandem repeat analysis for chimerism testing. Bone Marrow Transplant 2001; 28: 511–518.

    Article  CAS  Google Scholar 

  20. Herzog EL, Chai L, Krause DS . Plasticity of marrow-derived stem cells. Blood 2003; 102: 3483–3493.

    Article  CAS  Google Scholar 

  21. Grove JE, Bruscia E, Krause DS . Plasticity of bone marrow-derived stem cells. Stem Cells 2004; 22: 487–500.

    Article  Google Scholar 

  22. da Silva Meirelles L, Chagastelles PC, Nardi NB . Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci 2006; 119: 2204–2213.

    Article  Google Scholar 

  23. Tögel F, Westenfelder C . Adult bone marrow-derived stem cells for organ regeneration and repair. Dev Dyn 2007; 236: 3321–3331.

    Article  Google Scholar 

  24. Körbling M, Estrov Z, Champlin R . Adult stem cells and tissue repair. Bone Marrow Transplant 2003; 32 (Suppl 1): S23–S24.

    Article  Google Scholar 

  25. Hung EC, Shing TK, Chim SS, Yeung PC, Chan RW, Chik KW et al. Presence of donor-derived DNA and cells in the urine of sex-mismatched hematopoietic stem cell transplant recipients: implication for the transrenal hypothesis. Clin Chem 2009; 55: 715–722.

    Article  CAS  Google Scholar 

  26. Mayntz-Press KA, Ballantyne J . Performance characteristics of commercial Y-STR multiplex systems. J Forensic Sci 2007; 52: 1025–1034.

    Article  CAS  Google Scholar 

  27. Pujal JM, Gallardo D . PCR-based methodology for molecular microchimerism detection and quantification. Exp Biol Med 2008; 233: 1161–1170.

    Article  CAS  Google Scholar 

  28. Linch CA, Smith SL, Prahlow JA . Evaluation of the human hair root for DNA typing subsequent to microscopic comparison. J Forensic Sci 1998; 43: 305–314.

    Article  CAS  Google Scholar 

  29. Gross AM, Liberty AA, Ulland MM, Kuriger JK . Internal validation of the AmpFlSTR Yfiler amplification kit for use in forensic casework. J Forensic Sci 2008; 53: 125–134.

    Article  CAS  Google Scholar 

  30. Malsom S, Flanagan N, McAlister C, Dixon L . The prevalence of mixed DNA profiles in fingernail samples taken from couples who co-habit using autosomal and Y-STRs. Forensic Sci Int Genet 2009; 3: 57–62.

    Article  CAS  Google Scholar 

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Acknowledgements

We are greatful to Professor Geoffrey Shaw for his language editing of this paper.

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Authors and Affiliations

  1. Department of Forensic Medicine, Forensic Genetics Laboratory, Medical University of Lodz, Lodz, Poland

    R Jacewicz & J Berent

  2. Department of Hematology, Poznan University of Medical Sciences in Poland, Poznan, Poland

    K Lewandowski, J Rupa-Matysek & M Komarnicki

  3. Department of Forensic Court and Insurance Certification, Medical University of Lodz, Lodz, Poland

    M Jedrzejczyk & S Szram

  4. Department of Cytophysiology, Histology and Embryology, Medical University of Lodz, Lodz, Poland

    P M Brzezinski

  5. Department of Forensic Medicine, Molecular Techniques Unit, Wroclaw Medical University, Wroclaw, Poland

    T Dobosz & A Jonkisz

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  1. R Jacewicz
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  2. K Lewandowski
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  3. J Rupa-Matysek
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  4. M Jedrzejczyk
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Correspondence to R Jacewicz.

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Jacewicz, R., Lewandowski, K., Rupa-Matysek, J. et al. Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant 45, 1638–1644 (2010). https://doi.org/10.1038/bmt.2010.27

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