Circulating resistin levels and risk of multiple myeloma in three prospective cohorts

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Resistin is a polypeptide hormone secreted by adipose tissue. A prior hospital-based case–control study reported serum resistin levels to be inversely associated with risk of multiple myeloma (MM). To date, this association has not been investigated prospectively.


We measured resistin concentrations for pre-diagnosis peripheral blood samples from 178 MM cases and 358 individually matched controls from three cohorts participating in the MM cohort consortium.


In overall analyses, higher resistin levels were weakly associated with reduced MM risk. For men, we observed a statistically significant inverse association between resistin levels and MM (odds ratio, 0.44; 95% confidence interval (CI) 0.24–0.83 and 0.54; 95% CI 0.29–0.99, for the third and fourth quartiles, respectively, vs the lowest quartile; Ptrend=0.03). No association was observed for women.


This study provides the first prospective evidence that low circulating resistin levels may be associated with an increased risk of MM, particularly for men.


Multiple myeloma (MM), a plasma cell disease, was newly diagnosed in an estimated 24 280 individuals in 2016 in the United States (Teras et al, 2016). Besides well-established risk factors, such as male sex, older age, African ancestry, and a family history of haematological malignancies (Baris et al, 2013), obesity has been shown to increase MM risk (Teras et al, 2014). The association between obesity and MM may be attributable in part to altered levels of various adipokines (e.g., adiponectin, leptin, and resistin) secreted by adipose tissue (Dalamaga et al, 2009). We have recently shown that the risk of MM is increased for individuals with low pre-diagnosis circulating levels of adiponectin (Hofmann et al, 2016), but not of leptin (Hofmann et al, 2012). The potential role of resistin in MM development is poorly understood. An increased risk of MM for individuals with low serum levels of resistin was observed by one small hospital-based case–control study (73 MM cases and 73 controls) (Dalamaga et al, 2009). To our knowledge, the relationship between resistin and MM has not been evaluated prospectively. Using a nested case–control study involving three cohorts participating in the MM cohort consortium, we investigated whether pre-diagnosis circulating levels of resistin were associated with future MM risk.

Materials and methods

Cases (n=178) and controls (n=358) were selected from the Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study (ATBC), the Cancer Prevention Study II (CPS-II), and the Melbourne Collaborative Cohort Study (MCCS) (ATBC, 1994; Calle et al, 2002; Giles and English, 2002). Participating cohorts received Institutional Review Board approval from their respective institutions. We selected cases with a primary incident diagnosis of MM (ICDA=203; ICD-O-2-M=9731, 9732, and 9830; or ICD-O-3-M=9731–9734) and a stored blood sample collected 3 months before MM diagnosis. Controls with no history of cancer were individually matched to cases based on cohort of origin, birth year (±12 months), sex, type of blood sample (serum, plasma-heparin, and plasma-EDTA), date of blood collection (±2 months), and fasting status at blood draw with a 2 : 1 ratio as previously described (Birmann et al, 2012; Hofmann et al, 2016). All of the cases and controls included in this investigation were non-Hispanic whites.

Circulating levels of resistin were measured in duplicate by ELISA (Human Resistin Quantikine ELISA by R&D Systems, Inc., Minneapolis, MN, USA). Assays were performed in the laboratory of MNP at McGill University. The lower limit of detection was 0.156 ng ml−1. Samples from MM patients and their matched controls were analysed in the same batch, and blinded quality control (QC) replicates were included in each batch as previously described (Hofmann et al, 2016). The overall intraclass correlation coefficient was 0.86, and the coefficient of variation for pooled QC specimens was 6.1%, with no evidence of laboratory drift across cohorts.

We used the Wilcoxon rank-sum test to assess differences in resistin levels between cases and controls. For our main analyses, we performed cohort- and sex-specific corrections of resistin values to reduce the impact of cohort-related variability, and to account for differences between men and women within and across the participating studies (Birmann et al, 2012; Hofmann et al, 2016) (Supplementary Methods). Odds ratios (ORs) and 95% confidence intervals (95% CIs) for risk of MM were computed using conditional logistic regression models with levels of resistin categorised into quartiles based on the distribution among controls. We performed analyses stratified by sex, time to MM diagnosis, and age at sample collection using conditional logistic regression models, and analyses stratified by BMI categories using unconditional logistic regression models adjusted for matching factors. Multiplicative interaction was assessed using Wald tests. Study-specific ORs and 95% CIs were estimated comparing resistin levels above and below the median (based on the distribution in controls); we conducted a meta-analysis using both fixed and random effects models, and assessed heterogeneity across cohorts using the Cochran Q-test and the I2 statistic. For all analyses, findings were considered statistically significant if the two-sided P-value was <0.05.


Cases and controls had similar distributions of sex, age at the blood draw, and type of blood sample (Table 1). Cohort- and sex-corrected resistin levels were somewhat lower among cases compared with controls; these differences were statistically significant among men (P=0.006, Wilcoxon rank-sum test) but not among women (P=0.22). Levels of resistin and total adiponectin (measured previously) were not correlated among cases or controls (Spearman’s correlation coefficients of −0.07 and 0.09, respectively; P0.1). In multivariate analyses of the pooled controls, circulating levels of resistin were higher among men compared with women, but were not associated with age or BMI (Supplementary Table S1).

Table 1 Selected characteristics of cases and controls

Higher resistin levels were weakly associated with a reduced risk of MM in overall analyses, although the observed associations were not statistically significant (Table 2). Findings were similar after adjusting for BMI and adiponectin levels. Among a subset of participants from the ATBC and MCCS cohorts with available data on markers related to IGF-1 and interleukin (IL)-6 pathways (Birmann et al, 2012), we found that results were essentially unchanged after adjustment for these other analytes (data not shown). Among men, we observed a statistically significant reduced risk of MM among those with higher levels of resistin (ORs of 0.44 (95% CI 0.24–0.83) and 0.54 (0.29–0.99) for the third and fourth quartiles, respectively, vs the lowest quartile; Ptrend=0.026). This association remained after adjusting for BMI and adiponectin levels (data not shown). In contrast, resistin levels were not associated with MM risk among women (Pinteraction=0.12). In other stratified analyses, we did not observe statistically significant differences in the ORs across strata for time to MM diagnosis, BMI category, or age at blood collection. Our main findings were essentially unchanged after excluding high outlying resistin levels and when quartiles were based on the overall distribution of resistin levels among controls rather than the cohort- and sex-corrected levels.

Table 2 Circulating resistin levels and risk of MM, overall and stratified by selected characteristics

Cohort- and sex-specific results are shown in Figure 1. Among men, there was some evidence of heterogeneity across cohorts (I2=62.3%, P=0.071). The association was strongest among men in the MCCS (OR 0.12, 95% CI 0.026–0.53), although non-significant inverse associations were also observed among men in the other cohorts (ORs of 0.63 (0.30–1.34) and 0.79 (0.42–1.48) in CPS-II and ATBC, respectively). Among women, ORs were elevated but not statistically significant in both CPS-II and MCCS.

Figure 1

Cohort-specific and meta-analysis summary ORs and 95% CIs for the association between circulating resistin levels (above vs below the median) and MM risk among men (upper panel) and women (lower panel).


This study is, to our knowledge, the first prospective investigation of circulating levels of resistin and risk of MM. Our findings suggest that high pre-diagnosis circulating resistin levels are associated with a reduced future risk of MM development, in particular among men. These results are consistent with findings from a prior hospital-based case–control study (Dalamaga et al, 2009). Although we did not find evidence of an association among women, our investigation included relatively few female MM cases and thus we had limited statistical power for this subgroup analysis. Our findings may also reflect true differences by sex in the biological activity of resistin due to interaction with sex or metabolic hormones or other inflammatory markers that are differentially expressed in men and women (Dalamaga et al, 2013). We also observed a somewhat stronger association during the time period closer to MM diagnosis, which may reflect altered resistin expression related to onset or progression of monoclonal gammopathy of undetermined significance.

Resistin increases nuclear factor kappa B-related monocyte expression of pro-inflammatory cytokines such as IL-6 (Tilg and Moschen, 2006; Lee et al, 2014). Thus, the observed increased risk of MM in males with lower levels of resistin might reflect a compensatory effect of resistin or a negative feedback loop following the production of IL-6 and/or other cytokines with known effects on proliferation and survival of MM cells (Dalamaga et al, 2009). Alternatively, previous studies have shown that TNF-α decreases resistin secretion in murine models (Rajala et al, 2002). If TNF-α, a well-characterised pro-inflammatory cytokine that stimulates MM cell proliferation (Hideshima et al, 2001), also acts as a negative regulator of resistin in humans, this action may explain the observed inverse association between pre-diagnosis resistin levels and MM risk. This hypothesis is corroborated by increased resistin levels in MM patients after treatment with thalidomide, a TNF-α inhibitor (Reseland et al, 2009).

Resistin deregulation in obesity-related inflammatory conditions may also result from altered expression by non-adipocyte inflammatory cells, such as macrophages, in adipose tissue (Patel et al, 2003; Curat et al, 2006). While some studies have observed elevated levels of resistin among individuals with higher BMI or visceral fat area (Yannakoulia et al, 2003; Reseland et al, 2009), other studies found no association (Lee et al, 2003; Dalamaga et al, 2013). The lack of association of resistin with BMI in our study suggests that resistin may influence MM risk through other inflammatory processes. Notably, resistin is also highly expressed in the bone marrow (BM) where it is secreted by osteoclasts and osteoblasts, as well as by monocytes and macrophages (Patel et al, 2003). Resistin activates osteoclastogenesis and promotes osteoblast proliferation (Thommesen et al, 2006), which suggests a role for resistin in bone remodelling. Considering the interplay between the BM microenvironment and the proliferation of MM cells (Eda et al, 2016), impaired resistin expression in the BM niche may induce changes that are favourable to the proliferation of MM cells, which might explain the observed inverse association between resistin and MM risk. Functional analyses in BM tissue could help to clarify the role of resistin and other adipokines in MM pathogenesis.

The strengths of our study include its prospective design and the pooling of data across three cohorts. Limitations include the lack of detailed information on clinical characteristics of MM at diagnosis, as well as information on family history of lymphohaematopoietic malignancies. Given that having a family history of these relatively rare malignancies is uncommon in the general population (Schinasi et al, 2016), the potential for confounding by family history is likely to be minimal. However, further investigation in family-based studies and other studies with detailed clinical records may be informative. Finally, although this study is the largest to date that has evaluated the association between resistin and MM, we had limited statistical power for analyses restricted to women, as well as for the cohort-specific and other stratified analyses. Extending this investigation to other prospective cohorts is required to confirm our findings and to better elucidate the differences by sex in the relationship between resistin and MM.

Change history

  • 10 October 2017

    This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication


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This work was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute. The American Cancer Society funds the creation, maintenance, and updating of the Cancer Prevention Study-II cohort. MNP’s laboratory is funded in part by a grant from the Terry Fox Foundation, Vancouver, Canada. The ATBC Study is supported by the Intramural Research Program of the U.S. National Cancer Institute, National Institutes of Health, and by U.S. Public Health Service contract HHSN261201500005C from the National Cancer Institute, Department of Health and Human Services. The MMCC was first established in part with funds from NIH/NCI extramural grants K07 CA115687 (BMB) and R01 CA127435 in addition to the aforementioned support from the NCI Intramural Research Program. BMB is currently supported in part by R21 CA198239.

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Correspondence to Jonathan N Hofmann.

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Santo, L., Teras, L., Giles, G. et al. Circulating resistin levels and risk of multiple myeloma in three prospective cohorts. Br J Cancer 117, 1241–1245 (2017).

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  • multiple myeloma
  • resistin
  • prospective cohorts

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