Abstract
To obtain suitable cell lines for the immortalisation of human lymphocytes, we constructed a heteromyeloma between the murine myeloma Ag8 and human lymphocytes from a highly malignant polymorphic, centroblastic B-cell lymphoma. The thioguanine-resistant and HAT-sensitive heteromyeloma HAB-1 neither secretes nor contains cytoplasmatic immunoglobulins, the cells being EBV negative but positively stained for HLA-BC and the human proliferation marker Ki-67. The karyotype consists of about 50 murine and 20 human chromosomes. The HAB-1 cells grow in suspension and have a doubling rate of about 25-30 h. In fusion experiments with spleen cells from stomach carcinoma patients HAB-1 cells show a 5-7 times higher fusion efficiency than murine Ag8 cells or another heteromyeloma SPM4-0 and give stable antibody producing products. The cell line will be made available to interested scientists.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 24 print issues and online access
$259.00 per year
only $10.79 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Faller, G., Vollmers, H., Weiglein, I. et al. HAB-1, a new heteromyeloma for continuous production of human monoclonal antibodies. Br J Cancer 62, 595–598 (1990). https://doi.org/10.1038/bjc.1990.336
Issue Date:
DOI: https://doi.org/10.1038/bjc.1990.336
This article is cited by
-
Monoklonale Antikörper
Der Onkologe (2005)
-
Characterization of four new gastric cancer cell lines
Virchows Archiv B Cell Pathology Including Molecular Pathology (1993)