Abstract
The HT29R human colonic adenocarcinoma cell line grows as locally invasive, mucin-secreting tumours in immunosuppressed mice with a doubling time of 6 days. These tumours may be disaggregated to give single-cell suspensions with plating efficiencies of 25-45% in technically simple in vivo-in vitro cell-survival assays. The effect of maximum tolerated doses of 5-fluorouracil, melphalan and cyclo-phosphamide on in situ growth is only slight. in vivo-in vitro cell-survival assays phosphamide on in situ growth is only slight. in vivo-in vitro cell-survival assays are consistent with these in situ results. The relative ease of experimental manipulation and the high clonogenic efficiency of this tumour make it a useful addition to human tumour xenograft models.
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Warenius, H., Bleehen, N. In vivo-in vitro clonogenic assays in a human tumour xenograft with a high plating efficiency. Br J Cancer 46, 45–50 (1982). https://doi.org/10.1038/bjc.1982.163
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DOI: https://doi.org/10.1038/bjc.1982.163
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