Abstract
The subcellular distribution of either [14C] or [3H]3′-methylcholanthrene was studied in rat liver following a single intraperitoneal injection of the labelled hydrocarbon 10 hours previously.
Adsorbed or non-covalently bound methylcholanthrene and its metabolic derivatives occurred in all cell fractions studied with the exception of purified cell walls. The highest specific activities (d.p.m./mg. protein) were found in washed mitochondria, microsomes and ribosome-free microsomal membranes.
Covalent binding of methylcholanthrene and its metabolic derivatives to different cell fractions of rat liver occurs to a small extent and is considered not to be significant. The highest degree of binding occurs in washed mitochondria, microsomes, ribosome-free microsomal membranes and their constituent core proteins.
Cell sap which contains non-covalently bound 3′-methylcholanthrene was fractionated into pH 5 enzyme and pH 5 supernatant fractions. The pH 5 enzyme fraction which possesses a high specific activity (d.p.m./mg. protein) was further fractionated with ammonium sulphate into three fractions. The 0-30% ammonium sulphate fraction had the highest specific activity.
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Jones, P., Hawtrey, A. Studies on the Binding and Distribution of Radioactively Labelled 3′-Methylcholanthrene in Subcellular Fractions of Rat Liver. Br J Cancer 25, 845–852 (1971). https://doi.org/10.1038/bjc.1971.98
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DOI: https://doi.org/10.1038/bjc.1971.98