Abstract
Aim:
To determine if NOD2 is involved in host recognition of Aspergillus fumigatus (Af) conidia.
Methods:
An Af conidia pulmonary infection murine model was established by intranasal inoculation of Af onidia suspensions. Protein levels of NOD2 in lung tissue were determined by immunohistochemistry. A549 and phorbol-12-myristate 13-acetate (PMA)-activated THP-1 cell lines were treated with heat-killed Af conidia, then the presence of NOD2 protein in these cell lines was detected by Western blotting. The ability of muramyl dipeptide (MDP) to induce the secretion of TNF-α after incubation with heat-killed Af conidia was measured by enzyme-linked immunosorbent assay.
Results:
The expression of NOD2 protein in lung tissue increased after Af conidia infection. Heat-killed Af conidia significantly upregulated NOD2 protein expression in A549 cells and PMA-activated THP-1 cells. Additionally, Af conidia in conjuction with MDP, significantly increased the secretion of TNF-α in A549 cells and PMA-activated THP-1 cells.
Conclusion:
Af conidia upregulates NOD2 protein expression in vitro and in vivo. These findings indicate that NOD2 protein may respond to Af conidia.
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Project supported by the National Natural Science Foundation of China (No 30571542) and Shanghai Leading Academic Discipline Project (Project Number: B 115)
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Zhang, Hj., Qu, Jm., Shao, Cz. et al. Aspergillus fumigatus conidia upregulates NOD2 protein expression both in vitro and in vivo. Acta Pharmacol Sin 29, 1202–1208 (2008). https://doi.org/10.1111/j.1745-7254.2008.00860.x
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DOI: https://doi.org/10.1111/j.1745-7254.2008.00860.x
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