Abstract
Aim:
To investigate the direct effects of resistin and resistin-binding peptide (RBP) on lipid metabolism and endocrine function in adipose tissue.
Methods:
Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein (rResistin) or combined with RBP of varying concentrations(1×10−12mol/L, 1×10−10mol/L, 1×10−8mol/L). Free fatty acids (FFA) released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-α and adiponectin were detected by ELISA kit and RT-PCR respectively.
Results:
The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-α in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1×10−8mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations.
Conclusion:
RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.
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Project supported by grants from the National Natural Science Foundation of China (No 30371502), the Natural Science Foundation of Jiangsu Province (No BK2001120), and a grant from Jiangsu Province Health Department (No RC2002061).
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Gu, N., Han, Sp., Fei, L. et al. Resistin-binding peptide antagonizes role of resistin on white adipose tissue. Acta Pharmacol Sin 28, 221–226 (2007). https://doi.org/10.1111/j.1745-7254.2007.00480.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00480.x
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