Abstract
Aim:
To study the effects of propyl gallate on the interaction of tumor necrosis factor-α (TNF-α) with its soluble receptor, sTNFR-I.
Methods:
Interactions between TNF-α and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-α and sTNFR-I was then recorded.
Results:
sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm2. TNF-α then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-α and sTNFR-I in a dose-dependent manner.
Conclusion:
The binding between TNF-α and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.
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Project supported by the National Natural Science Foundation of China (No 90209054).
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Li, J., Huang, Jd., Wu, By. et al. Effects of propyl gallate on interaction between TNF-α and sTNFR-I using an affinity biosensor. Acta Pharmacol Sin 26, 1212–1216 (2005). https://doi.org/10.1111/j.1745-7254.2005.00198.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00198.x