Abstract
Aim:
To study the protein and mRNA expressions of pro-inflammatory and antiinflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract (GbE) on expressions of cytokines.
Methods:
The experimental model of AS in rats were established by intraperitioneal injection of vitamin D3 with high fat/cholesterol diet. GbE 100 mg/kg was administered to rats by ig. After 8 weeks, the expressions of IL-1β, TNF-α, IL-10, and IL-10R in the brain tissues of AS rats were detected by enzyme-linked immunosorbant assay, immunohistochemistry, Western blotting, and reverse transcriptase polymerase chain reaction.
Results:
The protein and mRNA expressions of IL-1β, TNF-α, and IL-10 in the brains were markedly higher in AS groups than that in control groups (6.11±0.15, 1.55±0.14, 0.54±0.04 ng/g wet weight vs 0.80±0.14, 0.33±0.09, and 0.33±0.02 ng/g wet weight, respectively). The protein and mRNA expressions of IL-1β and TNF-α in the brains were markedly lower in GbE groups (3.82±0.54, 0.95±0.08 ng/g wet weight) than that in AS groups, the protein and mRNA expressions of IL-10 and IL-10R in the brains were markedly higher in GbE groups (0.85±0.06 ng/g wet weight) than that in AS groups.
Conclusion:
GbE inhibited production of pro-inflammatory cytokines IL-1β and TNF-α, but upregulated the production of anti-inflammatory cytokines, IL-10 and IL-10R in brain, which might be related with its anti-AS actions.
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Mallat Z, Heymes C, Ohan J, Faggin E, Leseche G, Tedgui A . Expression of interleukin-10 in advanced human atherosclerotic plaques: relation to inducible nitric oxide synthase expression and cell death. Arterioscler Thromb Vasc Biol 1999; 19: 611–6.
Huang ZY, Yang PY, Almofti MR, Yu YL, Rui YC, Yang PY . Comparative analysis of the proteome of left ventricular heart of arteriosclerosis in rat. Life Sci 2004; 75: 3103–15.
Qiu Y, Rui YC, Li TJ, Zhang L, Yao PY . Inhibitory effect of extracts of Ginkgo biloba leaves on VEGF-induced hyperpermeability of bovine coronary endothelial cells in vitro. Acta Pharmacol Sin 2004; 25: 1306–11.
Hansen MK, Taishi P, Chen Z, Krueger JM . Cafeteria feeding induces interleukin-1β mRNA expression in rat liver and brain. Am J Physiol 1998: 274: R1734–9.
Adams JK, Tepperman BL . Colonic production and expression of IL-4, IL-6, and IL-10 in neonatal suckling rats after LPS challenge. Am J Physiol Gastrointest Liver Physiol 2001: 280: G755–62.
Mathurin P, Xiong S, Kharbanda KK, Veal N, Miyahara T, Motomura K, et al. IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells. Am J Physiol Gastrointest Liver Physiol 2002; 282: G981–90.
de Maat MP, Kluft C . The association between inflammation markers, coronary artery disease and smoking. Vascul Pharmacol 2002; 39: 137–9.
Wang YN, Che SM, Ma AQ . Clinical significance of serum cytokines IL-1β, sIL-2R, IL-6, TNF-α, and IFN-γ in acute coronary syndrome. Chin Med Sci J 2004; 19: 120–4.
Balbay Y, Tikiz H, Baptiste RJ, Ayaz S, Sasmaz H, Korkmaz S . Circulating interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha, and soluble ICAM-1 in patients with chronic stable angina and myocardial infarction. Angiology 2001; 52: 109–14.
Opal SM, DePalo VA . Anti-inflammatory cytokines. Chest 2000; 117: 1162–72.
Mallat Z, Besnard S, Duriez M, Deleuze V, Emmanuel F, Bureau MF, et al. Protective role of interleukin-10 in atherosclerosis. Circ Res 1999; 85: 17–24.
Smith DA, Irving SD, Sheldon J, Cole D, Kaski JC . Serum levels of the antiinflammatory cytokine interleukin-10 are decreased in patients with unstable angina. Circulation 2001; 104: 746–9.
Kleijnen J, Knipschild P . Ginkgo biloba. Lancet 1992; 340: 1136–9.
Lin JY, Cheng FC, Chung SY, Lin MC . Ginkgo biloba extract (EGb761) and FK506 preserve energy metabolites in the striatum during focal cerebral ischemia and reperfusion in gerbils monitored by microdialysis. J Biomed Sci 2004; 11: 611–6.
Sun BL, Zhang J, Wang XC, Xia ZL, Yang MF, Zhang SM, et al. Effects of extract of Ginkgo biloba on spasms of the basilar artery and cerebral microcirculatory perfusion in rats with subarachnoid hemorrhage. Clin Hemorheol Microcirc 2003; 29: 231–8.
Chung SY, Wang MF, Lin JY, Lin MC, Liu HM, Cheng FC . Effect of one week treatment with Ginkgo biloba extract (EGb761) on ischemia-induced infarct volume in gerbils. Am J Chin Med 2003; 31: 533–42.
Guillon JM, Rochette L, Baranes J . Effects of Ginkgo biloba extract on 2 models of experimental myocardial ischemia. Presse Med 1986; 15: 1516–9.
Chen J, Wang X, Zhu J, Shang Y, Guo X, Sun J . Effects of Ginkgo biloba extract on number and activity of endothelial progenitor cells from peripheral blood. J Cardiovasc Pharmacol 2004; 43: 347–52.
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Project supported by the National Natural Science Foundation of China (No 30271509).
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Jiao, Yb., Rui, Yc., Li, Tj. et al. Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract. Acta Pharmacol Sin 26, 835–839 (2005). https://doi.org/10.1111/j.1745-7254.2005.00106.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00106.x
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