Multifunctional upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite: a near-infrared light-triggered theranostic platform for imaging-guided photodynamic therapy

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Abstract

Photodynamic therapy (PDT) is an excellent therapeutic modality for various malignant and nonmalignant cancers. This approach utilizes reactive oxygen species generated through the reaction between photosensitizer and oxygen in tissues upon light irradiation to achieve effective treatment. However, limited penetration depth and oxygen-deficient microenvironment hinder the efficiency of PDT. In this work, we design a multifunctional near-infrared (NIR)-triggered theranostic agent based on upconversion–nanoparticles-Polyoxyethylene bis (amine)–trismethylpyridylporphyrin–fullerene nanocomposite (UCNP–PEG–FA/PC70) for imaging (fluorescence/upconversion luminescence/magnetic resonance imaging)-guided photodynamic therapy. In this multimodal nanocompsite, UCNPs are employed as light transducers to convert NIR light into ultraviolet–visible light to activate PC70 to generate singlet oxygen (1O2) even under low-oxygen conditions. Meanwhile, the upconversion emission, magnetic resonance imaging and fluorescence signal coming from UCNPs and PC70 nanocomposite enable UCNP–PEG–FA/PC70 to act as a multimodal imaging diagnostic agent, which facilitates the imaging-guided PDT. Furthermore, folate-mediated active targeting would enhance the accumulation of multifunctional hybrid in tumor. In vitro as well as in vivo results suggest that this smart nanocomposite is promising as an NIR light-triggered and -targeted theranostic platform for imaging-guided PDT of cancer, which may provide a solution to the bottleneck problems of PDT, namely, limited penetration depth and oxygen-deficient microenvironment.

Introduction

Theranostic nanoparticles, which combine both therapeutic and diagnostic capabilities in one nanoplatform, have potential to propel the biomedical field toward personalized medicine.1, 2, 3 Specifically, phototriggered theranostics, combining phototherapies (for example, photodynamic,4, 5 photothermal,6, 7 and phototriggered chemo8, 9/gene therapy10) with real-time photodiagnostics (for example, bioluminescence,11 fluorescence,12, 13 and photoacoustic imaging14, 15) have been actively pursued due to the advantages of spatiotemporal selectivity, non-ionizing radiation and specificity for disease destruction. Among them, near-infrared (NIR) light-triggered photodynamic theranostics, in which nanoparticles are used as photodynamic therapy (PDT) agents, have recently been receiving much attention due to deep penetration and simultaneous generation of reactive oxygen species (ROS) upon the NIR light irradiation, leading to ablation of cancer cells as a result. Up to date, several nanocomposites including gold nanocomposites,16, 17, 18, 19 carbon nanostructures,20, 21 W18O49,22 WS2,23 porphyrin-functionalized porous nanoparticles,24 and upconversion nanoparticles (UCNPs)25 as NIR light-triggered photodynamic theranostic nanoplatforms have shown efficacy in vitro and in vivo studies. Wherein, NIR light-triggered photodynamic theranostics based on lanthanide-doped UCNPs has been well investigated for biomedical uses due to their intrinsic anti-Stokes upconversion luminescent properties.26, 27, 28 Compared with traditional optical biomarkers, such as quantum dots and organic dyes, UCNPs exhibit excellent photostability, low background autofluorescence, remarkable penetration depth and high conversion efficiency for NIR photons into visible and ultraviolet (UV) ones, which lead to its unique superiority for bioimaging and bioassay studies.29, 30 In particular, NaGdF4-based UCNPs have been developed as multimodal imaging probes for upconversion luminescence (UCL) imaging and magnetic resonance imaging (MRI) modality.31 Such multifunctional nanomaterials offer synergetic advantages of UCL imaging, that is, deep penetration and high sensitivity, along with the anatomic imaging capability of MRI in high spatial resolution.31 Benefiting from the UV and visible emissions on specific NIR excitation, UCNPs have also been employed as light transducers to activate photosensitizers. UCNP-based PDT nanocomposites are considered promising platforms for cancer therapy due to the non-invasive manner and deep tissue penetration of NIR light, which breaks the dilemma of using UV or visible lights as irradiation source. Since Zhang et al.32 first demonstrated the application of UCNP-based nanocomposite for PDT of bladder cancer cells, recent advances in the integration of UCNPs with PSs such as chlorin e6, Rose Bengal, and IR825 (an NIR-absorbing dye) for their PDT applications in vitro and in vivo have created many excitements.33, 34, 35 However, the phototoxic effect of 1O2 on PDT is restricted because of a hypoxic condition in tumor tissue.36 Therefore, it is important to develop NIR light-triggered and -targeted theranostic platform for imaging-guided PDT of cancer under low-oxygen conditions.

Porphyrins as most common used PSs have been approved by Food and Drug Administration for treatment of various non-malignancies and malignancies. They have excellent photophysical properties, such as stronger absorption bands and high singlet oxygen quantum yield because of their large π-conjugated aromatic domains. Strong fluorescence of porphyrin also can be used as imaging probe to monitor the pharmacokinetics.37 Very recently, trismethylpyridylporphyrin-C70 (PC70) dyad has been reported to act as a promising photosensitizer for its reasonable water dispersibility, high photostability and favorable biocompatibility.38 Significantly, the present PC70 exhibits extraordinary PDT efficiency in vitro even under low-oxygen conditions due to its extremely long lifetime triplet state (211.3 μs).38 Unfortunately, the UV-to-blue excitation of PC70 hinders the enhancement of PDT efficiency in vivo due to the limited penetration depth of UV-to-blue irradiation. The introduction of UCNPs may pave the way for the application of PC70 as a NIR light-triggered PDT agent in vivo since UCNPs can serve as phototransducers by absorbing NIR light to activate PC70 to generate 1O2. In addition, the involved UCNPs may reduce the effect by energy transfer from UCNPs to porphyrin,38 and recover part of the quenched fluorescence of porphyrin.37

Herein, we design a multifunctional theranostic nanoplatform based on UCNPs/PC70 nanocomposite for NIR-triggered fluorescence/UCL/MR trimodal imaging and simultaneous PDT both in vitro and in vivo (Figure 1). To match the UV-to-blue absorption of PC70 for high efficiency of PDT, β-NaGdF4:Yb,Tm@NaGdF4 core/shell nanoparticles were rationally designed. Subsequently, PC70 was decorated on the surface of UCNPs through electrostatic interaction. Finally, the UCNPs/PC70 nanocomposite was coated with folic acid-modified PEG for active targeting and prolonged circulation time. With such a UCNP–PEG–FA/PC70 nanocomposite, trimodal imaging of FL/UCL/MR can be used to guide the PDT treatment of cancer cells efficiently in vitro and in vivo via the activation of PC70 by UV and blue emissions from UCNPs. Besides, folate as a target element can enhance accumulation of nanocomposite in tumor to improve the efficiency of PDT. Most importantly, the synthesized UCNP–PEG–FA/PC70 may provide solutions to the bottleneck problems of PDT, namely, limited penetration depth and oxygen-deficient microenvironment.

Figure 1
figure1

Schematic strategies of trimodal imaging guided UCNP–PEG–FA/PC70 (upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite) for photodynamic therapy.

Materials and methods

Instruments

Samples for transmission electron microscopy (TEM) analysis were prepared by drying a colloidal solution containing nanoparticles on amorphous carbon-coated copper grids. Low-resolution TEM was operated on a JEOL-JEM 2100 transmission electron microscope at 200 kV. High-resolution TEM was carried out on a JEOL-JEM 2100F field emission transmission electron microscope at 200 kV. Powder X-ray diffraction patterns were recorded on a Rigaku D/MAX-2000 diffractometer (Tokyo, Japan) with a slit of 1/2° at a scanning rate of 2° min/min, using Cu Kα radiation (λ=0.5406 Å). Upconversion emission spectra were recorded on a Hitachi F-4500 spectrometer, with the Photomultiplier tubes (PMT) voltage of 700 V. The particle size and zeta potential were determined by dynamic light scattering using Malvern Nano-ZS90 apparatus (Malvern Instruments, Worcestershire, UK). Absorbance measurements were carried out using UV2550 spectrophotometer (SHIMADZU, Shimane, Japan). Fluorescence spectra were acquired though Fluorolog spectrophotometer. IR spectra were measured using Thermo instrument (NICOLET In10 MX, Shanghai, China).

Materials

NH2-PEG3000-NH2 and folate were purchased from Innochem (Beijing, China). 1,10-Diamino-4,7-dioxadecane, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Acros (Shanghai, China). The culture medium Dulbecco’s modified Eagle’s medium (Invitrogen, Shanghai, China) supplemented with 10 % fetal bovine serum (Hyclone Company, South Logan, UT, USA), penicillin (100 μg ml−1) and streptomycin (100 μg ml−1) (Gibco, Grand Island, NY, USA). Dil, MitoRed, Lyso, PI and Hoechst 33258 were purchased from PuJingKangLi (Beijing, China). All other chemicals used were analytical reagents.

Synthesis of β-NaGdF4:Yb,Tm@NaGdF4

The β-NaGdF4:Yb,Tm@NaGdF4 core/shell nanoparticles were synthesized with a modified thermolysis method. Three steps are included as following.

Synthesis of α-NaGdF4:Yb,Tm nanoparticles (α-core)

A given amount of Ln(CF3COO)3 (1 mmol), 0.29 mmol Gd(CF3COO)3, 0.70 mmol Yb(CF3COO)3, and 0.01 mmol Tm(CF3COO)3, and CF3COONa (1 mmol) were added to a mixture containing oleic acid (OA), oleylamine (OM), and octadecene (ODE) (40 mmol, molar ratio: 1:1:2) into a three-necked flask (100 ml) at room temperature. The slurry was heated to 110 °C to remove low-boiling water, and oxygen with vigorous stirring under vacuum. Then, the solution was heated to 310 °C at a heating rate of 15 °C min−1 and kept for 15 min under N2 atmosphere. After cooling to room temperature, the nanoparticles were collected with centrifugation (7800 r.p.m., 10 min) after precipitation by adding excess amount of ethanol. The final product was dispersed in 10 ml of cyclohexane.

Synthesis of β-NaGdF4:Yb,Tm nanoparticles (β-core)

Five milliliter of as-prepared α-NaGdF4:Yb,Tm nanoparticles were redispersed in a mixture of OA and ODE (40 mmol, molar ratio: 1:1). Then, Ln(CF3COO)3 (0.5 mmol, 0.145 mmol Gd(CF3COO)3, 0.35 mmol Yb(CF3COO)3, and 0.005 mmol Tm(CF3COO)3), and CF3COONa (0.5 mmol) were added. After removing low boiling cyclohexane, water and oxygen with vigorous stirring under vacuum, the mixture was then heated to 325 °C at a heating rate of 15 °C min−1 and kept for 60 min under N2 atmosphere. Then, the reaction was cooled to room temperature. Excess amount of ethanol was added to precipitate the nanoparticles, which was collected by further centrifugation (7800 r.p.m., 10 min) and redispersed in 10 ml of cyclohexane.

Synthesis of β-NaGdF4:Yb,Tm@NaGdF4 nanoparticles (β-core@shell)

Five milliliter of as-prepared β-NaGdF4:Yb,Tm nanoparticles were redispersed in a mixture of OA and ODE (40 mmol, molar ratio: 1:1). Then, Gd(CF3COO)3 (1 mmol) and CF3COONa (1 mmol) were added. The slurry was heated to 110 °C to remove low boiling cyclohexane, water, and oxygen with vigorous stirring under vacuum. Then, the solution was heated to 320 °C at a heating rate of 15 °C min−1 and kept for 40 min under N2 atmosphere. After cooling to room temperature, the nanoparticles were collected with centrifugation (7800 r.p.m., 10 min) after precipitation by adding excess amount of ethanol. The final product was dispersed in 5 ml of cyclohexane.

Preparation of citric acid modified nanoparticles (citric-UCNP)

One milliliter cyclohexane dispersions containing hydrophobic nanoparticles were precipitated with ethanol by centrifugation and redispersed in 5 ml of chloroform. Citric acid (200 mg) was dissolved in 5 ml of deionized water. The pH value of the citric acid solution was tuned ~7 by ammonia. Subsequently, the two solutions were mixed under vigorous stirring at room temperature overnight. After that, the chloroform phase was deserted, whereas the water phase was transferred to a centrifuge tube. Citric acid-modified UCNPs were collected after high-speed centrifugation at 18 000 r.p.m. for 15 min and then washed with water for several times. Finally, the products were redispersed in water.

Synthesis of folate-NH-PEG3000-NH2

Folate–NH–PEG3000–NH2 conjugate was synthesized according to the previous method. Briefly, folate was activated with EDC and NHS at a 1:2:2 molar ratio in dimethyl sulfoxide for 2 h. Under nitrogen atmosphere, folate was filtered with a syringe filter (pore size: 0.2 μm). A hetero-bifunctional poly (ethylene glycol) derivate (NH2–PEG3000–NH2 Mw 3000) was added to the above solution at a molar ratio of 15:1 (folate:PEG). After reaction for 4 h under nitrogen atmosphere, the reactants were precipitated with acetone and centrifuged to remove the folate residue. Folate-NH–PEG3000–NH2 conjugation in the supernatant was dialyzed against deionized water.

Synthesis of UCNP-ca/PC70 (UCNP/PC70)

Citric-UCNP (37.6 mg) was dispersed in water (10 ml). Then 18.8 mg of PC70 was added into the above solution and stirred overnight to obtain UCNP/PC70.

Synthesis of UCNP–NH–PEG3000–NH–folate/PC70 (UCNP–PEG–FA/PC70)

Fifteen milligram of EDC and 8.5 mg of NHS were added into the above UCNP/PC70 solution and stirred for 2 h. Then, folate–NH–PEG3000–NH2 (500 mg) was added into the above solution, followed by vigorous stirring for 24 h. The nanoparticles were dialyzed by deionized water for 1 week.

Synthesis of PC70

In brief, terephthalaldehydic acid methyl ester, pyridine-4-aldehyde and pyrrole were reacted in propionic acid for 1.5 h, and the resultant mixture was separated through a silica gel. Then LiAlH4 was used to reduce the ester group to primary hydroxyl group at room temperature. Subsequently a Swern oxidation reaction was applied to obtain aldehyde group, the final product was purified by silica gel column chromatography. After that, Prato reaction was carried out to obtain crude product which was subjected to silica gel column chromatography. The obtained final product was mixed with methyl tosylate and refluxed in argon atmosphere for 1 h and then passed through an anion-exchange resin repeatedly to yield PC70 as a chloride salt.

Loading capacities and release behaviors PC70

A gradient concentration (4, 8, 12, 16, 20 and 25%) of PC70 in UCNPs solutions was centrifuged at 5000 r.p.m. min−1 for 30 min. PC70 loading content was calculated according to the following formula: PC70 loading content=weight of PC70/(weight of PC70+weight of UCNPs). The release behavior of PC70 from the nanocomposites was evaluated in 10% fetal bovine serum. The resulting solution was filtrated through ultrafiltration (M.W.cut off=10 000) and the filtrate was detected by ultraviolet absorbance spectra.

Detection of singlet oxygen from UCNP–PEG–FA/PC70

Water soluble disodium 9,10-anthracendipropionic acid (Na2–ADPA) was used as the 1O2 trapping agent. In the experiments, 100 μl of Na2–ADPA solution (1 mg ml−1) was added into 1.5 ml of UCNP–PEG–FA/PC70 solution and then exposed to 980 laser irradiation at a power density of 1 W cm−2. To eliminate the inner-filter effect, the maxima absorption of UCNP–PEG–FA/PC70 was adjusted to about 0.2 OD. The absorption of Na2–ADPA from 380 to 420 nm was recorded at different irradiation times.

In vitro PDT

The photo-cytotoxicity of UCNP–PEG–FA/PC70 in vitro was carried out against luciferase-expression cervical cancer (HeLa-luc). Hela-luc cells were seeded in a 96-well plate with density of ca. 5 × 104 per well and cultured in 5% CO2 at 37 °C for 24 h. Hela-luc cells were then treated with UCNP–PEG–FA/PC70 in different concentration gradients for 3 h in the dark, and then irradiated with a 980 nm laser for 10 min (0.8 W cm−2, 1 min break after 1 min irradiation) under air condition and nitrogen condition, respectively. Afterward, the aged medium was replaced with the fresh medium, and cells were cultivated for 24 h in the dark at 37 °C. Cytotoxicity was evaluated by a WST-8 assay with a Cell Counting Kit-8 (CCK-8; DOJINDO, Kumamoto, Japan). A 96-well plate reader (iMarkmicroplate reader, Bio-RAD, Hercules, CA, USA) was used to determine cell viability. Hela-luc cells treated only with UCNP–PEG–FA/PC70 in the dark for 24 h were used as control. As for other controls, three kinds of cells including Hela-luc cells, A549 and HBE cells were treated with either UCNP–PEG/PC70 (156 μg ml−1) or PC70 (40 μg ml−1, 23.8 μM) and UCNP (80 μg ml−1). Cell viability was then detected. To further confirm the targeting property of UCNP–PEG–FA/PC70, folate blocking experiment was done by pre-incubated Hela-luc, A549 and HBE cells with folate (40 μg ml−1) for 0.5 h. The cells were further incubated with UCNP–PEG–FA/PC70 (200 μg ml−1) for 3 h. Afterward, the cell viability was detected by CCK-8. Corresponding results are expressed as mean±s.d.

Cell imagings

Hela-luc cells were incubated with UCNP–PEG–FA/PC70 (200 μg ml−1) at 37 °C, 5% CO2 for 3 h. The aged cell culture was replaced with the fresh Dulbecco’s modified Eagle’s medium (without phenol red). Subsequently, the cells were illuminated by an external 980 nm (0.8 W cm−2, 1 min break after 1 min irradiation) laser for 10 min. Either Hoechst 33258 (1 mg ml−1, 5 μl, incubated with cells for 20 min), PI (1 mg ml−1, 5 μl, incubated with cells for 15 min), Dil (1 mg ml−1, 5 μl, incubated with cells for 20 min), MitoRed (1 mg ml−1, 5 μl, incubated with cells for 30 min), or Lyso (1 mg ml−1, 5 μl, incubated with cells for 45 min) was then added according to the actual conditions. The cells were washed with icy phosphate-buffered saline buffer for three times before observation. The images were obtained by FV 1000-IX81 confocal laser scanning microscope (Olympus, Miyazaki, Japan). The cells were dyed without laser irradiation as a control.

In vivo PDT

Female Balb/c nude mice (16–20 g) were purchased from Center for Experimental Animals, Institute of Process Engineering, Chinese Academy of Science (Beijing, China). In vivo study conforms to the guidelines of The National Regulation of China for Care and Use of Laboratory Animals. The tumor model was established by subcutaneous injection of Hela/luc cells in the right buttock of each mouse. The tumors were allowed to grow for around 5 days to reach the size of ca. 30–60 mm3.

The tumor-bearing mice were randomized into six groups (n=3, each group). Group 1 was treated by intravenous injection with UCNP–PEG–FA/PC70 at a dosage of 200 μl (2 mg ml−1). After 24 h post-injection, the tumor sites were irradiated with 980 nm NIR light for 3 min (0.7 W cm−2, 30 s break after 30 s irradiation). The other three groups were intravenously injected with UCNP–PEG–FA (1.6 mg ml−1, 200 μl), PC70 (0.4 mg ml−1, 200 μl), and saline (200 μl), respectively, and followed the same procedure as controls. The fifth and sixth groups were treated with UCNP–PEG–FA/PC70 (2 mg ml−1, 200 μl) and saline (200 μl) without laser irradiation as another control. All the groups were treated with only one injection. The body weights and tumor volumes were monitored every 2 days after treatment. The tumor volume was calculated using the following equation: tumor volume (V)=length × width2/2. Relative tumor volume was calculated as V/V0 (V0 was the corresponding tumor volume when the treatment was initiated).

Trimodal FL/UCL/MR imaging in vivo

Fluorescence imaging was performed on an in vivo imaging system (CRI Maestro 2) with a 600 nm laser as the excited source. The fluorescence images were corrected at 560–800 nm and analyzed with Maestro2 software. The Balb/c nude mice were anesthetized with 1.5% pentobarbital sodium by intraperitoneal injection at dosage of 100 μl. UCNP–PEG–FA/PC70 nanoparticles (200 μl, 2 mg ml−1) were injected by intravenous injection, and fluorescence imaging was performed at timed intervals (0, 2, 4, 8, 24 and 72 h).

In vivo UCL imaging experiments were performed with a CALIPER Lumina II in vivo imaging system with an adjustable 980 nm laser as the excited source for in vivo imaging. Before imaging, the Balb/c nude mice were anesthetized with 1.5% pentobarbital sodium by intraperitoneal injection at dosage of 100 μl. UCNP–PEG–FA/PC70 nanocomposites (200 μl, 2 mg ml−1) were injected by intravenous injection, and UCL imaging was performed after 24 h post-injection with exposure time of 0.3 s.

The in vitro MR imaging experiments were performed in a 0.5 T MRI magnet (NM120-Analyst, Shanghai Niumag Corporation). UCNP–PEG–FA/PC70 nanocomposites were dispersed in water at various Gd concentrations (by ICP measurement). T1 was acquired using an inversion recovery sequence. Finally, the r1 value was determined through the curve fitting of 1/T1 relaxation time (s−1) versus the Gd concentration (mM).

In vivo MR imaging experiments were performed on a 7 T MRI scanner (Micro MRI, Varian 7T). The tumor-bearing mouse was anesthetized with isoflurane. The mouse was scanned before and after 24 h intravenous injection of UCNP–PEG–FA/PC70 nanoparticles (200 μl, 4 mg ml−1).

Histopathological examination

The tissues (heart, liver, spleen, kidneys and lung) were harvested and fixed in a 4% formalin solution. The histopathological tests were performed according to standard laboratory procedures. Tissue samples were numbered and given blind to the pathologist for conventional processing and analysis. Briefly, the tissue samples were embedded in paraffin blocks, sectioned into 5 μm slices, and mounted onto glass slides. The sections were observed after hematoxylin and eosin staining, and photographs were taken using an optical microscope.

Results and Discussion

Synthesis and characterization of UCNP–PEG–FA/PC70

PC70 dyad was synthesized according to our previously reported paper.25 (Supplementary Scheme S1, step 1). To obtain prominent UV–visible emission, NaGdF4:Yb,Tm@NaGdF4 core/shell nanoparticles were prepared as the upconversion energy donor. Moreover, Gd3+ ions-based host lattice was employed to support the MRI capability by shortening the relaxtion time of T1. The synthesis of the core/shell UCNPs was enabled with a modified thermolysis method (Supplementary Scheme S1, step 2).39, 40

As given in Supplementary Figure S1a, the core and core/shell UCNPs were sphere-like, with high uniformity in size and morphology. After epitaxial growth of the nanoshells (Figure 2a), the mean particle size increased by ~20 nm. Furthermore, the high-resolution TEM image of core/cell nanoparticles (Figure 2b) exhibited legible lattice fringes of 0.52 nm, indicating the plane of β-NaGdF4, whereas the successful formation of β-phase UCNPs was also confirmed by the X-ray diffraction patterns (Figure 2d). The upconversion spectra in Supplementary Figure S2 show that the emission intensity of NaGdF4:Yb,Tm@NaGdF4 core/shell UCNPs enhanced 13 times compared with the NaGdF4:Yb,Tm counterpart, indicating the higher upconversion efficiency of the core/shell UCNPs. Afterward, a ligand exchange strategy was employed to transfer the hydrophobic oleate-capped UCNPs to aqueous solutions, where the oleate ligands were replaced by citric acid molecules. As shown in Supplementary Figure S3, no obvious change in size and morphology of the UCNPs occurred after the ligand exchange process. Meanwhile, the emission intensity of Tm3+ decreased due to the quenching effect induced by the vibration stretching of hydroxyl groups (Supplementary Figure S4).

Figure 2
figure2

(a) Transmission electron microscope (TEM) image of β−NaGdF4:Yb,Tm@NaGdF4 nanoparticles. (b) High-resolution TEM image of β−NaGdF4:Yb,Tm@NaGdF4 nanoparticle. (c) TEM images of UCNP–PEG–FA/PC70 (upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite). Inset is enlarged image and the scale bar is 5 nm. (d) X-ray diffraction pattern of as-prepared β-NaGdF4:Yb,Tm@NaGdF4 nanoparticles, consistent with the standard pattern of β-NaGdF4 (JCPDS 06-0342). (e) Optical photographs of UCNP–PEG–FA/PC70 in water, phosphate-buffered saline, fetal bovine serum and Dulbecco’s modified Eagle’s medium respectively: before centrifugation (top) and after centrifugation (bottom). (f) Absorption spectra of UCNP–PEG–FA/PC70, UCNPs, FA and PC70. (g) Upconversion emission spectrum of UCNPs and absorption spectrum of PC70. Inset is the digital luminescence photograph of UCNPs. (h) Upconversion emission spectra of UCNP–PEG–FA solutions treated with PC70 at different concentrations upon 980 nm irradiation (excitation power was 4 W cm−2). The concentrations of UCNPs in samples are identical. Inset is a local amplification profile of spectra. (i) Photodegradation of Na2-ADPA treated with UCNP–PEG–FA/PC70 at different time points due to generation of singlet oxygen upon 980 nm irradiation.

Subsequently, water-dispersible and carboxyl-functionalized UCNPs were modified by PC70 via electrostatic interactions and then encapsulated by folate-modified polyoxyethylenebis (amine). TEM image shows little change in size of the UCNPs after modification by PEG and PC70 (Figure 2c), which may be caused by light atoms with lower constrast compared with that of heavy atoms. Whereas the dynamic light scattering distribution indicates that the hydrated ionic radius is ca. 186 nm, which may ascribe to the free stretching of PEG (Supplementary Figure S5). Further investigation illustrates that the as-prepared UCNP–PEG–FA/PC70 nanocomposites are reasonably stable in phosphate-buffered saline, fetal bovine serum and Dulbecco’s modified Eagle’s medium (Figure 2e), which should mainly attribute to the negative charged property (−15 mV) of UCNP–PEG–FA/PC70. The moderate dispersibility facilitates further biomedical applications. The IR spectrum indirectly verifies the formation of amido bond (Supplementary Figure S6). Loading efficiency of PC70 was measured as shown in Supplementary Figure S7a, and the result suggested that the loading efficiency is ca. 20%. Release behavior of PC70 was also evaluated in 10% fetal bovine serum (Supplementary Figure S7b). PC70 could release from the nanocomposite with an amount of ca. 5–7% indicating that the multifunctional composite is reasonably stable under physiological conditions.

Figure 2g reveals that the absorption spectrum of PC70 overlaps with the UV emission of UCNPs at 290 nm (1I63H6), 345 nm (1I63F4), 361 nm (1D23H6), and the visible emission at 451 nm (1D23F4), 475 nm (1G43H6), indicating that the emissions of UCNPs matched well with the absorption of PC70. Hence, PC70 can be activated by the energy transfer from UCNPs upon NIR irradiation. To further verify the efficient energy transfer from the UCNPs to PC70, the emission spectra of UCNP–PEG–FA nanoparticles incubated with different concentration of PC70 were monitored upon 980 nm irradiation. As depicted in Figure 2h, the UV–visible emissions of Tm3+ were progressively quenched with elevated addition of PC70, whereas the emission of Tm3+ at 800 nm (3H43H6) was almost constant, indicating that the energy transfer indeed occurred between UCNPs and PC70 due to the overlap of emission and absorption spectra in UV–visible range. As a result, a slight red-shift of the UV–visible absorption at 428 nm (Figure 2f) and an obvious red-shift of the typical FL spectrum of PC70 (Supplementary Figure S8) were observed for UCNP–PEG–FA/PC70 due to the interaction between UCNPs and PC70. These results indirectly indicate that fluorescence resonance energy transfer may exist between UCNPs and PC70. Subsequently, the feasibility of UCNP–PEG–FA/PC70 for producing 1O2 was investigated by using chemical trapping method (Figure 2i). The result indicates that the photodegradation of Na2–ADPA is time-dependent once treated with UCNP–PEG–FA/PC70 and 980 nm irradiation. All of these results indicate that the as-synthesized UCNP–PEG–FA/PC70 nanocomposites not only exhibit reasonable stability under physiological conditions but also efficiently produce singlet oxygen upon 980 nm irradiation.

The photodynamic activity in vitro

To investigate the NIR light-triggered PDT effect of UCNP–PEG–FA/PC70, the in vitro viability assay of Hela-luc cells was performed under normal conditions and N2-puring conditions (simulate a low oxygen condition) as a model. As given in Figure 3a, the cell viabilities decrease gradually with the increase of nanoparticles from 0 to 200 μg ml−1 upon 980 nm laser irradiation at a power density of 0.8 W cm−2 (480 J cm−2, lower than that of previously reported: 4.5 W cm−2, 10 800 J cm−2),8 and 90% inhibitory effect on the cell viability was detected at 0.15 mg ml−1 of UCNP–PEG–FA/PC70. Meanwhile, negligible cytotoxicity against the Hela-luc cell was observed in the dark, indicating the favorable biocompatibility of UCNP–PEG–FA/PC70 nanocomposites. The same experiments were done under the N2-puring conditions. As shown in Figure 3b, the cell viabilities decrease gradually with the increase of UCNP–PEG–FA/PC70 from 0 to 200 μg ml−1 under N2-puring condition, and 50% inhibitory effect on the cell viability was detected at 0.2 mg ml−1. The results indicate that UCNP–PEG–FA/PC70 can produce 1O2 under low-oxygen conditions, which may be attributable to the formation of (3PC70)* with a long triplet lifetime state and thus have enough time to interact with free oxygen.38

Figure 3
figure3

(a) In vitro relative viabilities of Hela-luc cells incubated with UCNP–PEG–FA/PC70 (upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite) at different concentrations, with (red) and without (black) 980 nm NIR light irradiation. (b) Relative viabilities of Hela-luc cells under nitrogen-purging conditions incubated with UCNP–PEG–FA/PC70 at different concentrations, with (red) and without (black) 980 nm NIR light irradiation. (c) Confocal images before (left) and after (right) NIR light irradiation. First line was stained with DiI and Hoechst 33 258, second line was stained with MitoRed and Hoechst 33258, third line was stained with PI and Lyso. Each group with three columns represent fluorescence image, optical image and merged image, respectively, from left to right.

Several subcellular organelles were stained by related probes and observed under confocal microscope to confirm that the cells were destructed after incubation with UCNP–PEG–FA/PC70 and subsequent irradiation with 980-nm NIR light. As shown in Figure 3c, the images in the first row were stained by Dil (cell membrane dye) and Hoechst 33258 (nucleus dye), from which we can see clearly that the cell membrane was bubbling and the nucleus was stained in blue after 980-nm irradiation, indicating that the cell membrane was destroyed and the cells died. The second row was stained by MitoRed tracker (mitochondrial dye) and Hoechst 33258, and the third row was stained by Lyso tracker (lysosome dye) and PI (nucleus dye). The compact mitochondria and lysosomes become incomplete after irradiation, indicating that the subcellular organelles were destroyed by 1O2 generated from UCNP–PEG–FA/PC70 after 980 nm irradiation. The lightened subcellular organelles indirectly illustrated that the nanocomposites were internalized into cells and produced intracellular ROS to destroy the subcellular organelles when exposed to NIR light irradiation.

Improved photodynamic activity by conjugation with folate

To reduce the photodynamic damage of synthesized nanoparticles to normal cells and enhance the PDT efficiency toward tumor cells, folate-modified UCNP–PEG–FA/PC70 was designed. As shown in Figure 4a, UCNP–PEG–FA/PC70 hybrid exhibits a dramatic decrease in cell viability even at low concentration of 0.2 mg ml−1, whereas UCNPs or PC70 alone showed a negligible effect under the same conditions. Moreover, A549 and HBE cells with negative expression of folate receptor were used as controls to evaluate the PDT efficiency. The results indicate that all the above materials including UCNP–PEG–FA/PC70 show negligible cytotoxicity toward A549 and HBE cells, suggesting that the superior PDT effect should benefit from the higher binding affinity of the folate-modified nanoprobe to folate receptor overexpressed Hela-luc cells.

Figure 4
figure4

(a) The viabilities of three kinds of cells (Hela-luc, A549 and HBE cells) incubated with UCNP–PEG–FA/PC70 (upconversion-nanoparticles-trismethylpyridylporphyrin-fullerene nanocomposite), UCNP–PEG/PC70, UCNP–PEG–FA, PC70 and UCNPs respectively. (b) The viabilities of three kinds of cells (Hela-luc, A549 and HBE cells) incubated with either UCNP–PEG–FA/PC70 or the mixture of UCNP–PEG–FA/PC70 and an excess of folate. (c) Confocal images of PI stained Hela-luc cells (left) and A549 cells (right) after photodynamic therapy treatment with UCNP–PEG–FA/PC70, UCNP–PEG/PC70, UCNP and PC70 from top to bottom, respectively.

Competitive inhibition experiment was carried out to further confirm the targeting specificity of UCNP–PEG–FA/PC70. UCNP–PEG/PC70 (without folate) and UCNP–PEG–FA/PC70 blocking with an excess of folate during the incubation were used in PDT treatment as controls. As shown in Figure 4a, UCNP–PEG/PC70 exhibited negligible effect on the above three kinds of cells, suggesting that UCNP–PEG/PC70 fails to effectively attach to the cell surface at absence of folate. Moreover, the blocking group of UCNP–PEG–FA/PC70 reveals that the existence of free folate could inhibit the affinity of UCNP–PEG–FA/PC70 to Hela-luc cells and therefore effectively preserve the cells from PDT damage (Figure 4b). In addition, confocal imaging experiment was explored to further confirm the above results. Strong red fluorescence could be observed when the Hela-luc cells were treated with UCNP–PEG–FA/PC70 and subsequently exposed to 980-nm irradiation and stained by PI. Meanwhile, controls groups (UCNP–PEG–FA/PC70-treated A549 cells and UCNP–PEG/PC70-, UCNPs- and PC70-treated A549 cells, as well as HBE cells and Hela-luc cells) exhibited negligible fluorescence under the same conditions (Figure 4c). These results highlight the targeting capability and specificity of UCNP–PEG–FA/PC70 nanocomposites toward Hela-luc cells via the recognition between folate and folate receptor on the Hela-luc cell surface.

Fluorescence/UCL/MR/trimodal imaging

Despite the promising results obtained in vitro, one of the most demanding challenges regarding multifunctional platforms still lies in good sensitivity in vivo and real-time monitoring for optimized treatment. Herein, the fluorescence intensity in vivo was detected to evaluate the real-time biodistribution of UCNP–PEG–FA/PC70 nanoparticles. For in vivo optical imaging studies, tumor-bearing nude mice were injected with UCNP–PEG–FA/PC70 through the tail vein (20 mg kg−1). The resulting fluorescence avoids the autofluorescence from biological tissues and could be used to image the specifics sensitively in vivo. From the chronological observation of the images in Figure 5a, we detected the appearance of a rapid tropism associated with the biodistribution of UCNP–PEG–FA/PC70 nanoparticles. The fluorescence intensity of UCNP–PEG–FA/PC70 in tumor increases to the maximum after 24 h post injection and then decreases with the prolonging time. At the time point of 24 h, the tumor and other organs of the nude mouse injected with UCNP–PEG–FA/PC70 were harvested for ex vivo imaging to quantify tissue/organ distribution (Figure 5b). The result revealed that distinct fluorescence was presented in tumor site and also in the liver and kidney. By contrast, as given in Supplementary Figure S9, the mouse treated with UCNP–PEG/PC70 showed negligible fluorescence after 4 h post-injection. The ex vivo imaging obtained after 24 h post injection (Supplementary Figure S10) indicated the maximal accumulation in the liver and kidney and only a weak fringe of fluorescence located in tumor sites. These results demonstrate that the active targeting of folic and enhanced permeability and retention effect mediated passive targeting play a synergistic role for the accumulation of UCNP–PEG–FA/PC70 nanocomposites in tumor. Most interestingly, the real-time fluorescence images not only confirm the feasibility of fluorescence-guided PDT but also contribute to the enhanced efficiency of PDT.

Figure 5
figure5

(a) In vivo fluorescence images of a tumor-bearing mouse at different time points after treatment with UCNP–PEG–FA/PC70 (upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite). (b) UCL images at the time point of 24 h, and images from left to right represent optical image, UCL image, and merged image. Ex vivo images of tumor and other organs after 24 h post-injection: (c) fluorescence image and (d) UCL image. (e) Relaxation rate R1 versus different molar concentrations of UCNP–PEG–FA/PC70 nanoparticles. (f) In vitro T1-weighted MR images of UCNP–PEG–FA/PC70 at different Gd concentrations. (g) In vivo T1-weighted MR image of a tumor-bearing nude mouse: preinjection (left) and after 24-h post injection (right). The color images are treated by pseudo-color.

In addition, the emission of UCNPs at 800 nm could be used as the output signal to realize the NIR-to-NIR UCL imaging. The images from left to right in Figure 5c represent the optical image of mouse, UCL image, and their merged images at 24 h post injection, which depict the tumor clearly. The ex vivo UCL imaging (Figure 5d) further indicated that UCNP–PEG–FA/PC70 mainly accumulated in the liver, spleen and tumor, which were similar to the result from fluorescence image.

To demonstrate the MRI capability of UCNP–PEG–FA/PC70, T1 relaxation time and T1-weighted MR images at different Gd concentrations were obtained with a 0.5 T MRI scanner. The measured r1 value was ca. 3.0 mM–1 s–1 for UCNP–PEG–FA/PC70 nanocomposites (Figure 5e), which was comparable to that of clinically used Gd-DTPA (3–4 mM–1 s–1) under the same conditions. T1-weighted MR images (Figure 5f) enhanced with the increase of Gd concentration from 0 to 8 mM. For better visual effect, pseudocolor image was used to enhance the sensitivity. In vivo MR imaging was performed for a tumor-bearing mouse with a 7 T MR scanner (Figure 5g). The tumor site was clearly depicted after 24-h post injection, indicating that UCNP–PEG–FA/PC70 can be used as a potential T1 MRI contrast agent. The results from MR image are reasonably consistent with those from fluorescence and UCL images, which also indicate the better tumor targeting performance of UCNP–PEG–FA/PC70 as an entity. In a word, the obtained results indicate that UCNP–PEG–FA/PC70 could be employed as an efficient and trimodal FL/UCL/MR imaging agent in vivo and direct the optimized PDT treatment.

In vivo PDT

The PDT experiments were conducted in vivo to evaluate the tumor inhibition efficacy of the UCNP–PEG–FA/PC70 with 980 nm NIR light irradiation. The luciferase-expressing cervical cancer cell line Hela-luc was selected as the xenograft model, and tumor-bearing nude mice were randomly divided into six groups (n=3). The first group was irradiated with 980 nm NIR light (0.7 W cm−2, 126 J cm−2, 30 s break after 30 s irradiation) in the tumor site for 3 min (lower than that of previously reported: 2.5 W cm−2, 30 min, 4500 J cm−2)8 after injection with UCNP–PEG–FA/PC70 (2 mg ml−1, 200 μl) for 24 h through caudal vein. In addition, short-interval irradiation method was developed to avoid over-heating by the NIR light. The next three groups were injected with UCNP–PEG–FA, PC70, and saline, respectively, and then irradiated under the same conditions as controls. Another two groups injected with either UCNP–PEG–FA/PC70 or saline separately without irradiation were used as controls. Figure 6a demonstrates that the group treated with UCNP–PEG–FA/PC70+ 980 nm NIR light irradiation exhibited optimal tumor growth inhibition efficacy. By contrast, the tumors of control groups grew continuously under the same conditions. Meanwhile, the digital photographs of representative mice with neoplasm (Supplementary Figure S11) before and after irradiation for 6 days showed that the tumor sizes of mice treated with UCNP–PEG–FA/PC70+980 nm NIR light irradiation were much smaller than those of control groups, as confirmed by the representative bioluminescence images of mice (Supplementary Figure S12) given at the 14th day (Figure 6c). Bioluminescence from the UCNP–PEG–FA/PC70+980 nm NIR light irradiation-treated group nearly disappeared compared with saline-treated mice, suggesting that the tumor of treated group was effectively destroyed. Hematoxylin and eosin staining images of main organs and tumor are presented in Figure 6d. The clear separation of the nuclear and cytoplasm confirmed that the tumor cells were destroyed after treated with UCNP–PEG–FA/PC70+980 nm NIR light irradiation. By contrast, tumor from the mouse treated with saline exhibited cramped construction of nuclear and cytoplasm. Importantly, there were no obvious lesions were found in other organs, indicating that UCNP–PEG–FA/PC70 has no significant toxicity to organisms. In addition, body weight, as an important parameter to evaluate the systemic toxicity of nanomaterials, did not show abnormal change for all groups (Figure 6b), indicating that UCNP–PEG–FA/PC70 nanocomposite does not have obvious adverse side effects.

Figure 6
figure6

(a) Growth of tumors after treatments. The relative tumor volumes were normalized to their initial sizes. (b) The relative body weights of the nude mice increased continuously after different treatments. Error bars indicate standard deviations; n=3. (c) Bioluminescence images of different treatments after 14 days: UCNP–PEG–FA/PC70+980 nm NIR light irradiation (left) and saline+980 NIR light irradiation (right). (d) Hematoxylin and eosin stained images of tumors and other organs under different photodynamic therapy treatments.

Conclusion

In summary, we have successfully prepared UCNP–PEG–FA/PC70 nanocomposite as an NIR light-triggered and targeted theranostic platform for trimodal imaging-guided PDT in vitro as well as in vivo. The developed UCNP–PEG–FA/PC70 nanocomposites have the following advantages: (1) Upon NIR irradiation, UCNPs could convert NIR light into UV-to-visible light to effectively activate PC70 to produce 1O2 for killing tumor under low oxygen conditions; (2) Three kinds of imaging methods (FL/UCL/MR) were integrated into one system to provide the complementary information for accurate diagnosis and guide treatment of tumor; (3) Folate-mediated active targeting together with the PEG leaded enhanced permeability and retention effect effect (passive targeting) enhances the accumulation of multifunctional nanoparticles in tumor. Importantly, the synthesized UCNP–PEG–FA/PC70 may provide solutions to the two bottleneck problems of PDT, namely, limited penetration depth and oxygen-deficient microenvironment. These results highlight the potential of UCNP–PEG–FA/PC70 as a multifunctional theranostic agent for imaging-guided PDT of tumor and a promising candidate for overcoming the problem of existing PDT agents in deep cancerous tissue under hypoxic conditions.

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Acknowledgements

This work is supported by 973 Program (No. 2011CB302100), the Ministry of science and technology support program (No. 2011BAK15B04), the Chinese Academy of Sciences (No. XDA09030302), the National Natural Science Foundation of China (Nos. 51372251, 51472252) and the Key Research Program of the Chinese Academy of Sciences (Grant no. KGZDEW-T02).

Author contributions

CS and JG planned and supervised the project. MG carried out the detailed experiments. MG, JG and CS analyzed the data and wrote the whole paper. HD contributed to the synthesis and characterization of UCNPs and helped the analysis of the data. DC, LS, CY, CW and PW contributed to discussion of the paper.

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Correspondence to Chunying Shu.

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Supplementary Information accompanies the paper on the NPG Asia Materials website

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