Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion

Abstract

To assess the role of interleukin-4 (IL-4) and interleukin-13 (IL-13) in colon cancer cell–cell adhesion, we investigated the effect of both cytokines in human colon cancer cell line, colo205 cell–cell adhesion. IL-4 receptor was expressed on the cell surface of colo205, and recombinant IL-4 inhibited colo205 cell–cell adhesion in a dose-dependent fashion without inhibiting cell proliferation. Flow cytometric analysis revealed that monoclonal antibodies (mAbs) directed against E-cadherin and carcinoembryonic antigen (CEA) inhibited colo205 cell–cell adhesion and IL-4 significantly inhibited the expression of E-cadherin and CEA. IL-13 also inhibited colo205 cell–cell adhesion. These results indicated that IL-4 and IL-13 inhibited colon cancer cell–cell adhesion by down-regulation of E-cadherin and CEA molecules. We then investigated the expression of both cytokines from freshly isolated colon cancer tumour-infiltrating lymphocytes (TILs). With reverse transcription-polymerase chain reaction and flow cytometric analysis, we demonstrated that colon TILs expressed IL-4 and IL-13 mRNA and protein. These results suggest that Th 2 type cytokines IL-4 and IL-13 locally-produced from TILs may regulate colon cancer adhesion by down-regulation of adhesion molecules. © 2000 Cancer Research Campaign

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Kanai, T., Watanabe, M., Hayashi, A. et al. Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. Br J Cancer 82, 1717–1723 (2000) doi:10.1054/bjoc.2000.1113

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Keywords

  • IL-4
  • IL-13
  • colon cancer
  • cell adhesion

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