WEB-2086 – an antagonist of platelet-activating factor receptor (PAFR) with known anti-inflammatory, antiangiogenic and antileukaemic properties – also proved to inhibit the proliferation in human solid tumour cell lines of different histology, and with much higher efficacy than in normal fibroblasts. A detailed analysis of WEB-2086 anticancer activity was then performed focusing on breast adenocarcinoma MCF-7 and MDA-MB-231 cells. WEB-2086-treated cells, either expressing (MCF-7) or unexpressing (MDA-MB-231) the oestrogen receptor (ER)α, underwent a dose-dependent growth arrest (IC50=0.65±0.09 and 0.41±0.07 mM, respectively) and accumulation in G0–G1 phase. WEB-2086 also induced morphological and functional changes typical of mature mammary phenotype including (i) cell enlargement and massive neutral lipid deposition (best accomplished in MCF-7 cells); (ii) decrease in motility and active cathepsin D levels (mainly observed in highly invasive MDA-MB-231 cells). The expression of ERα was neither increased nor reactivated in treated MCF-7 or MDA-MB-231 cells, respectively. WEB-2086-induced differentiation in breast cancer cells involved the upregulation of PTEN, a key tumour suppressor protein opposing tumorigenesis, and was apparently independent of p53, PAFR, peripheral benzodiazepine receptor and ERα status. Overall, WEB-2086 can be proposed as an effective antiproliferative and differentiative agent with interesting translational opportunities to treat breast cancers in support to conventional chemotherapy.
Differentation-inducing therapy found its best clinical application by using all-trans-retinoic acid (ATRA) (Warrell et al, 1991) and STI571 (Druker, 2002) to cure successfully acute promyelocytic leukaemia (APL) and chronic myelogenous leukaemia, respectively. Such excellent effects, however, were not reproduced in other haematological and, particularly, in solid tumours, which, therefore, represent a real challenge for differentiative agents.
We showed previously that WEB-2086 – an antagonist of platelet-activating factor receptor (PAFR) with known anti-inflammatory properties (Ishii and Shimizu, 2000) – could also induce erythroleukaemia cell maturation (Cellai et al, 2002), enhance ATRA differentiation potential, trigger apoptosis in ATRA-sensitive and -resistant APL cell lines, and in blasts from patients with APL (Laurenzana et al, 2005). These results prompted us to investigate WEB-2086 effects also in solid tumour cells. We examined different cell lines from human breast adenocarcinoma, colon adenocarcinoma, hepatocarcinoma, fibrosarcoma, and neuroblastoma and found that the drug induced growth arrest in all types of cancer cells and with much higher efficacy than in normal cells.
WEB-2086 activity was then systematically analysed in MCF-7 and MDA-MB-231 breast adenocarcinoma cell lines. MCF-7 are low-migrating cells expressing oestrogen receptor (ER)α while MDA-MB-231 are ERα-negative cells with high mobility and invasive potential (Bussolati et al, 2000). These models have been widely used to test in vitro differentiation activity of agents like Na-butyrate and hexamethylene bisacetamide (HMBA) (Guilbaud et al, 1990), and retinoids including ATRA (Wang et al, 2001). More recently, emphasis was given to histone deacetylase inhibitors (HDACi) such as suberoylanilide hydroxamic acid (SAHA) and trichostatin A (TSA) which induce differentiation in breast cancer cells in vitro (Yang et al, 2000; Munster et al, 2001; Jang et al, 2004) by altering their transcriptional programs. However, the clinical use of HADCi including valproic acid to treat breast cancer is severely limited by the drug-mediated increase of ERα expression and tissue sensitization to tumour promotion activity of oestrogens (Akhmedkhanov et al, 2001; Graziani et al, 2003).
In this study we report that WEB-2086 exerted a broad cytostatic effect in cell lines from solid tumours of different histology. Moreover, with respect to particularly MCF-7 and MDA-MB-231 breast adenocarcinoma cells, WEB-2086 could re-establish cell growth and migration control regardless of ERα status, and restore also some traits of mature mammary phenotype without enhancing and/or reactivating ERα expression. All these properties point to WEB-2086 as a novel differentiation agent with a low-toxic profile in vivo (Adamus et al, 1989) and with interesting therapeutic potential for treating breast cancers in support to conventional cytotoxics.
Materials and methods
Cells, culture conditions and reagents
Media used (Bio-Whittaker Europe, Verviers, Belgium) were IMDM for MCF-7 and Hep-G2; DMEM for MDA-MB-231, HT-1080 and dermal fibroblasts; RPMI for SH-SY5Y (plus 1% non-essential AA) and HCT-8 (plus 1 mM Na-pyruvate). Cells were propagated in the presence of 10–15% foetal bovine serum (FBS, EuroClone, Life Science Division, Milan, Italy) and 2 mM L-glutamine at 37°C in 5% CO2 humidified atmosphere. Cells were harvested with a trypsin-EDTA solution (Bio-Whittaker Europe), plated (1.5 × 104 ml−1) and left for 6 h before any treatment. WEB-2086 (Casals-Stenzel et al, 1987) (Boehringer Ingelheim Pharma KG, Biberach, Germany), was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St Louis, MO, USA); the stock solution (260 mM) was stored in the dark at room temperature. HMBA, Na-butyrate and ATRA were from Sigma-Aldrich; other chemicals were reagent grade. Cells treated with increasing WEB-2086 for 72 h were harvested and counted in a Bürker chamber to assess the drug concentration necessary to reduce cell density by 50% as compared to control (IC50 value).
Cell cycle distribution was determined by the propidium iodide (PI)-hypotonic citrate method with a FACScan instrument (Becton-Dickinson, San Jose, CA, USA) (Paoletti et al, 1996).
The number of cell replications was assessed with the aid of carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes, Eugene, OR, USA), which covalently binds cell components to yield a fluorescence that was divided equally between daughter cells at each division (Lyons et al, 2001). Cells were starved for 14 h with the medium containing 0.5% FBS, then harvested and labelled with CFSE following the manufacturer's instruction. Labelled cells (day 0) were used to measure initial levels of fluorescence and then seeded (2 × 104 cells ml−1) with or without either WEB-2086 (0.5–1.5 mM) or DMSO as the vehicle (0.19–0.57%) to calculate replication number after 5 days of treatment (software: ModFit LT for Macintosh, Proliferation Protocol, Verity Software House Inc., Topsham, ME, USA).
Cell morphology, apoptosis and neutral lipid determination
Morphological changes were microscopically examined either by phase contrast or ethanol-fixed cells stained with haematoxylin-eosin using a Digital Camera System Leica DC 200 (Leica Microsystems, Inc. Bannockburn, IL, USA). Apoptosis was revealed by the presence of nuclear fragmentation in stained cells and by the DNA ladder assay as reported previously (Laurenzana et al, 2005).
Neutral lipids were determined (i) histochemically (Constantinou et al, 1998) on cell monolayers which were quickly fixed with −20°C methanol, stained with Oil Red O (Sigma-Aldrich) and counterstained with haematoxylin; or (ii) spectrophotometrically (Cary 50 Scan, Varian, Victoria, Australia) at 510 nm by recording absorbance of cell-bound Oil Red O following extraction with isopropanol (Wang et al, 2001).
Reverse transcription–polymerase chain reaction
Total RNA was prepared with TRIzol (Invitrogen, Carlsbad, CA, USA), following the manufacturer's instructions. Samples (1 μg each) were reverse-transcribed and then amplified by PCR with the following primers. For ERα, 5′-CAAGCCCGCTCATGATCA-3′ and 5′-CACCATGCCCTCTACACA-3′ (388 bp); for peripheral benzodiazepine receptor (PBR), 5′-CACGCTCTACTCAG-CCATGG-3′ and 5′-GCAGTAGTTGAGTGTGGTCGC-3′ (298 bp); for PAFR, 5′-ACCAACACAGTGCCCGACAGTGCT-3′ and 5′-GGGTGACCTGATGTGCATCA-TTAAT-3′ (363 bp); for β2-microglobulin, 5′-CTCGCGCTACTCTCT-CTTTCT-3′ and 5′-ACATGGAGACAGCACTCAAAG-3′ (514 bp). Reverse transcription-polymerase chain reaction (RT–PCR) products were analysed as described previously (Cellai et al, 2002).
Cell mobility was assessed in Boyden's chambers using a polyvinyl-pyrrolidone-free polycarbonate filter (8 μm pore size) between the two chambers (Alessandri et al, 1986). After a 6-h incubation at 37°C, cells (2.5 × 104, 0.2 ml−1) on the upper surface of filters were scraped; filters, were then fixed in methanol, stained with Wright-Giemsa and photographed as above.
SDS–PAGE and Western blotting
Harvested cells were resuspended in the lysis buffer (Laurenzana et al, 2005) and separated on 12.5% SDS–PAGE. Western-blot membranes were probed using primary antibodies against cathepsin D, p53, PTEN, and α-tubulin (Santa Cruz Biotech, Santa Cruz, CA, USA) and ERα (Ventana Medical Systems, Tucson, AZ, USA). Peroxidase-conjugated secondary antibodies (Santa Cruz Biotech) and the ECL procedure on Hyperfilm ECL (Amersham Pharmacia Biotech) were used for development.
All experiments were independently done at least three times. All data were statistically analysed by Student's t-test.
WEB-2086 inhibited proliferation in six different solid tumour cell lines
Human tumour cells from breast adenocarcinoma (MCF-7 and MDA-MB-231), ileocecal adenocarcinoma (HCT-8), hepatocarcinoma (Hep-G2), fibrosarcoma (HT-1080), and neuroblastoma (SH-SY5Y) were cultured with increasing WEB-2086 concentrations for 3 days. IC50 values of solid tumour cells were within the range of 0.38–0.83 mM WEB-2086 (mean value ∼0.61 mM) (Table 1). HT-1080 and HCT-8 were the more and the less drug-responsive cells, respectively, while normal human fibroblasts used as control yielded a mean IC50 value >4 mM. These data indicated that solid tumour cells, irrespective of their histogenesis, were all especially sensitive to WEB-2086-induced cytostasis thus inferring that a common pathway might be involved. WEB-2086 effects have been then analysed further in breast adenocarcinoma cells: MCF-7 which are hypotetraploid, ERα-positive and low-mobility cells expressing the wild-type p53 form; and MDA-MB-231 which are hypotriploid, ERα-negative and highly invasive cells carrying a mutated and inactive p53 form.
WEB-2086 induced growth arrest and differentiation in MCF-7 cells
WEB-2086-treated MCF-7 cells underwent a striking inhibition of growth that was virtually abolished at ⩾1 mM drug (Figure 1A). WEB-2086 effects on MCF-7 replicative potential was evaluated by tracking subsequent rounds of cell division with the fluorescent dye CFSE (see Materials and methods) after a 5-day incubation of cultures with or without increasing WEB-2086 concentrations (Figure 1B and legend). Approximately 75% of untreated MCF-7 cells approached the fourth generation and 23% reached the third generation. Conversely, in MCF-7 cultures incubated with 1 or 1.5 mM WEB-2086, the predominant fraction (67 and 92%, respectively) was represented by cells arrested after two replications only.
Cytofluorimetric analysis of cell cycle (Figure 1C) showed that a 2-day treatment led to a dose-dependent MCF-7 accumulation in G0–G1 phase to reach approximately 66 and 74% of population at 1 and 1.5 mM drug, respectively. Concomitantly, there was a decrease in G2-M/S cell fraction and a moderate increase in the sub-G1 fraction over basal levels. However, DNA ladder assay and morphology of treated cells indicated that apoptosis involved only a minor portion of the population (data not shown).
MCF-7 incubated with 1 mM WEB-2086 showed features that were reminiscent of mature mammary phenotype including an increase in cell size and eosinophilia, a reduced nuclear/cytoplasmic ratio, and a shift from a round to columnar shape to yield adenoma-like clusters where a central lumen was often recognized (Figure 2 left, B vs A). Red Oil O staining of treated MCF-7 cells revealed a massive accumulation of neutral lipids, which are an important milk component and the most typical trait of mature epithelial mammary cells (Figure 2 left, D and insert, vs C).
Cell density and neutral lipid levels were determined in MCF-7 incubated with increasing amounts of either WEB-2086 or HMBA given alone or in combination (Figure 2, right). WEB-2086 caused a marked dose-dependent decrease in cell density and increase in neutral lipid content of approximately twofold, fivefold and 22-fold over control levels after a 7-day incubation with 0.5, 1 and 1.5 mM drug, respectively. HMBA was much less efficient than WEB-2086 in inhibiting cell growth and inducing neutral lipid accumulation. However, the two inducers acted synergistically. The combinations of 0.5 mM WEB-2086 with either 1 or 3 mM HMBA inhibited MCF-7 cell growth and increased neutral lipid levels with higher efficacy than the two drugs alone. No synergy was observed between WEB-2086 and either Na-butyrate or ATRA (data not shown).
Response of MDA-MB-231 cells to WEB-2086
ERα-negative MDA-MB-231 cells were approximately 40% more sensitive than ERα-positive MCF-7 cells to drug-induced inhibition of cell growth (Figure 3A and Table 1). Treated MDA-MB-231 turned into scanty elongated and moderately enlarged fibroblast-like cells with no tendency to form bundles (Figure 3B). Cell cycle analyses showed that approximately 56 and 70% of MDA-MB-231 treated for 2 days with 0.5 and 1 mM WEB-2086, respectively, were arrested in G0–G1 phase; concomitantly, the G2-M/S cell fraction decreased while the sub-G1 fraction slightly increased (Figure 3C).
Neutral lipids were already detectable in untreated MDA-MB-231 cells but increased markedly upon WEB-2086 addition (Figure 3D) though at half the rate of MCF-7 cells.
WEB-2086 reduced breast cancer cell mobility and active cathepsin D levels
MDA-MB-231, unlike MCF-7, are cells with high mobility and have been used, therefore, to monitor WEB-2086 effects on cell migration and cathepsin D levels which associate closely with breast cancer invasiveness (Rochefort et al, 2000). Results of Boyden's chamber experiments (Figure 4A) showed that a 5-day incubation of MDA-MB-231 with 0.5 mM WEB-2086 decreased cell migration of approximately 75% while the low-migrating activity of MCF-7 was virtually unaffected by 1 mM drug. Moreover, WEB-2086 treatment of MDA-MB-231 decreased the active form of cathepsin D (lower band) and increased its inactive precursor protein (upper band) (Figure 4B); the latter was the only form present in low-invasive MCF-7 cells, irrespective of treatment.
Molecular and biochemical changes induced by WEB-2086 on PBR, PAFR, ERα, PTEN and p53 in MCF-7 and MDA-MB-231 cells
Drug effects on peripheral benzodiazepine receptor (PBR) mRNA levels in MCF-7 and MDA-MB-231 cells have been analysed; WEB-2086 was reported to bind these receptors (Svetlov et al, 1996) which are a hallmark of aggressive adenocarcinoma cells (Hardwick et al, 2001). After a 5-day treatment, PBR expression was downmodulated and, particularly, in highly invasive MDA-MB-231 cells (Figure 5A). Instead, PAFR expression was unaffected by the drug in both cell lines (data not shown).
The incubation of MCF-7 or MDA-MB-231 cells with WEB-2086 neither increased (actually, slightly decreased) nor restored ERα protein levels, respectively (Figure 5B). Importantly, an increase in PTEN protein relative to control was detected after a 2-day incubation with WEB-2086 in MCF-7 and, especially, in MDA-MB-231 cells (Figure 5C). Wild p53 levels in MCF-7 cells increased early in response to WEB-2086 with a peak at 3 h and then decreased progressively with time; instead, high levels of mutated p53 protein in MDA-MB-231 cells did not vary with treatment (Figure 5D).
Studies on WEB-2086 have primarily been performed with leukaemia cells that were induced to differentiation and/or apoptosis. Here we proved that WEB-2086 might exert a wider anticancer activity being capable to decrease significantly proliferation also in human solid tumour cells of different histogenesis and with much higher efficacy than in normal cells. Then we focused on breast adenocarcinoma cells lines and showed that WEB-2086 can effectively inhibit in vitro cell growth and migration as well as relieve the differentiation block in ERα-positive MCF-7 and ERα-negative MDA-MB-231 cells. WEB-2086-induced growth arrest developed rapidly and led to a dose-dependent cell accumulation in G0–G1 phase with only a moderate activation of apoptosis. On a molar basis, differentiation efficacy of WEB-2086 was from 6- to 12-fold higher than that of butyrates and HMBA (Guilbaud et al, 1990).
Drug-induced changes in morphology and neutral lipid accumulation were more promptly elicited in ERα-positive MCF-7 cells while the decrease in mobility and active cathepsin D levels was especially observed in highly migrating ERα-negative MDA-MB-231 cells. Importantly, WEB-2086 effects were reversed upon drug removal to suggest that for potential therapeutic purposes carcinoma cells should receive the drug continuously as reported for other differentiation inducers such as butyrates (Guilbaud et al, 1990), TSA (Jang et al, 2004) and SAHA (Munster et al, 2001). Moreover, specific HDACi including valproic acid increased and/or reactivated ERα expression (Yang et al, 2000; Graziani et al, 2003) and sensitize both ERα-positive and ERα-negative breast cancers to treatment with tamoxifen (Jang et al, 2004). However, tamoxifen acts as an antioestrogen in breast, but as an oestrogen in the uterus, bone and cardiovascular system (Sommer and Fuqua, 2001) and its administration was associated with endometrial tumour development (Akhmedkhanov et al, 2001; Graziani et al, 2003). Noteworthy, WEB-2086 did neither increase nor restore ERα expression in MCF-7 or MDA-MB-231 cells, respectively.
WEB-2086 action mechanisms in breast cancer cells are still unclear but it is unfeasible that p53 could be involved as MDA-MB-231 cells express a mutated and inactive protein form. Moreover, the relatively high WEB-2086 amounts required to induce adenocarcinoma cell cytostasis and differentiation seem to rule out a relevant role of classical PBR and PAFR. This hypothesis is in keeping with other studies reporting that ligands of PBR like PK11195 and Ro 5-4864 (Carmel et al, 1999), and of PAFR such as SDZ 62-434 (Brunton and Workman, 1993) and CV 3988 (Bussolati et al, 2000) inhibited breast cancer cell proliferation at micromolar levels which are not consistent with high-affinity ligand-receptor binding. Importantly, WEB-2086 induced the upregulation of PTEN protein in MCF-7 and, especially, in MDA-MB-231 cells. PTEN plays a key role in several tumours including breast cancers by contrasting the activation of proto-oncogenic phosphatydilinositol 3′ kinase (PI3K)-Akt signalling pathway and also tumorigenesis (Pandolfi, 2004; Chen et al, 2005). Increase in PTEN levels and cell accumulation in G0–G1 phase as observed in treated breast cancer cells might be part of a more general mechanism common to other tumour cell types that, irrespective of their histology, were all sensitive to WEB-2086-induced growth arrest.
Overall, we demonstrated that WEB-2086 (a) induced a dose-dependent cytostasis in a panel of human cell lines from distinct solid tumours; (b) promoted morphological and functional differentiation in both ERα-positive MCF-7 and ERα-negative MDA-MB-231 cells through, possibly, PTEN upmodulation; (c) did not enhance/reactivate ERα expression; (d) synergized with HMBA. Two final comments might emphasize further the translational opportunities of WEB-2086. First, the drug proved to be relatively safe in animal models and humans (Adamus et al, 1989) and produced negligible alterations of clinical parameters when administered to thrombocytopenic patients (Lohmann et al, 1988; Giers et al, 1990). Second, WEB-2086 capability to abrogate the PAF-mediated signal will contribute to reduce growth, tumorigenic and metastatic potential of transformed cells, and impact also cancer microenvironment by contrasting neoangiogenesis (Bussolati et al, 2000).
Adamus WS, Heuer H, Meade CJ, Brecht HM (1989) Safety, tolerability, and pharmacologic activity of multiple doses of new platelet activating factor antagonist WEB 2086 in human subjects. Clin Pharmacol Ther 45: 270–276
Akhmedkhanov A, Zeleniuch-Jacquotte A, Toniolo P (2001) Role of exogenous and endogenous hormones in endometrial cancer: review of the evidence and research perspectives. Ann NY Acad Sci 943: 296–315
Alessandri G, Raju KS, Gullino PM (1986) Interaction of gangliosides with fibronectin in the mobilization of capillary endothelium. Possible influence on the growth of metastasis. Invasion Metastasis 6: 145–165
Brunton VG, Workman P (1993) In vitro antitumour activity of the novel imidazoisoquinoline SDZ 62-434. Br J Cancer 67: 989–995
Bussolati B, Biancone L, Cassoni P, Russo S, Rola-Pleszczynski M, Montrucchio G, Camussi G (2000) PAF produced by human breast cancer cells promotes migration and proliferation of tumor cells and neo-angiogenesis. Am J Pathol 157: 1713–1725
Carmel I, Fares FA, Leschiner S, Scherübl H, Weisinger G, Gavish M (1999) Peripheral-type benzodiazepine receptors in the regulation of proliferation of MCF-7 human breast carcinoma cell line. Biochem Pharmacol 58: 273–278
Casals-Stenzel J, Muacevic G, Weber K (1987) Pharmacological actions of WEB 2086, a new specific antagonist of platelet activating factor. J Pharmacol Exp Ther 241: 974–981
Cellai C, Laurenzana A, Vannucchi AM, Della Malva N, Bianchi L, Paoletti F (2002) Specific PAF antagonist WEB-2086 induces terminal differentiation of murine and human leukemia cells. FASEB J 16: 733–735
Chen Z, Trotman LC, Shaffer D, Lin HK, Dotan ZA, Niki M, Koutcher JA, Scher HI, Ludwig T, Gerald W, Cordon-Cardo C, Pandolfi PP (2005) Crucial role of p53-dependent cellular senescence in suppression of PTEN-deficient tumorigenesis. Nature 436: 725–730
Constantinou AI, Krygier AE, Mehta RR (1998) Genistein induces maturation of cultured human breast cancer cells and prevents tumor growth in nude mice. Am J Clin Nutr 68: 1426S–1430S
Druker BJ (2002) STI571 (Gleevec) as a paradigm for cancer therapy. Trends Mol Med 8: S14–S18
Giers G, Janzarik H, Kempe ER, Mueller-Eckhardt C (1990) Failure of the platelet-activating-factor antagonist WEB 2086 BS for treatment of chronic autoimmune thrombocytopenia. Blut 61: 21–24
Graziani G, Tentori L, Portarena I, Vergati M, Navarra P (2003) Valproic acid increases the stimulatory effect of oestrogens on proliferation of human endometrial adenocarcinoma cells. Endocrinology 144: 2822–2828
Guilbaud NF, Gas N, Dupont MA, Valette A (1990) Effects of differentiation-inducing agents on maturation of human MCF-7 breast cancer cells. J Cell Physiol 145: 162–172
Hardwick M, Rone J, Han J, Haddad BR, Papadopoulos V (2001) Peripheral-type benzodiazepine receptor levels correlate with the ability of human breast cancer MDA-MB-231 cell line to grow in SCID mice. Int J Cancer 94: 322–327
Ishii S, Shimizu T (2000) Platelet-activatig factor (PAF) receptor and genetically engineered PAF receptor mutant mice. Prog Lipid Res 39: 41–82
Jang ER, Lim SJ, Lee ES, Jeong G, Kim TY, Bang YJ, Lee JS (2004) The histone deacetylase inhibitor trichostatin A sensitizes oestrogen receptor alpha-negative breast cancer cells to tamoxifen. Oncogene 23: 1724–1736
Laurenzana A, Cellai C, Vannucchi AM, Pancrazzi A, Romanelli MN, Paoletti F (2005) WEB-2086 and WEB-2170 trigger apoptosis in both ATRA-sensitive and -resistant promyelocytic leukemia cells and greatly enhance ATRA differentiation potential. Leukemia 19: 390–395
Lyons AB, Hasbold J, Hodgkin PD (2001) Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stably integrated fluorescent probe. Methods Cell Biol 63: 375–398
Lohmann HF, Adamus WS, Meade CJ (1988) Idiopathic thrombocytopenia treated with Paf-acether antagonist WEB 2086. Lancet 2: 1147
Munster PN, Troso-Sandoval T, Rosen N, Rifkind R, Marks PA, Richon VM (2001) The histone deacetylase inhibitor suberoylanilide hydroxamic acid induces differentiation of human breast cancer cells. Cancer Res 61: 8492–8497
Pandolfi PP (2004) Breast cancer – loss of PTEN predicts resistance to treatment. N Engl J Med 351: 2337–2338
Paoletti F, Mocali A, Cellai C, Caporale R, Vannucchi AM (1996) Megakaryocyte-like increase in ploidy of Friend's erythroleukemia cells induced to endoreplication by colcemid. Exp Hematol 24: 1441–1448
Rochefort H, Garcia M, Glondu M, Laurent V, Liaudet E, Rey JM, Roger P (2000) Cathepsin D in breast cancer: mechanisms and clinical applications, a 1999 overview. Clin Chim Acta 291: 157–170
Sommer S, Fuqua SA (2001) Estrogen receptor and breast cancer. Semin Cancer Biol 11: 339–352
Svetlov SI, Barton JM, Olson MS (1996) The specific binding of the platelet-activating factor (PAF) receptor antagonist WEB 2086 and the benzodiazepine flunitrazepam to rat hepatocytes. Life Sci 58: PL81–PL86
Wang Q, Lee D, Sysounthone V, Chandraratna RAS, Christakos S, Korah R, Wieder R (2001) 1,25-dihydroxyvitamin D3 and retinoic acid analogues induce differentiation in breast cancer cells with function- and cell-specific additive effects. Breast Cancer Res Treat 67: 157–168
Warrell Jr RP, Frankel SR, Miller Jr WH, Scheinberg DA, Itri LM, Hittelman WN, Vyas R, Andreeff M, Tafuri A, Jakubowski A, Gabrilove J, Gordon MS, Dmitrovsky E (1991) Differentiation therapy of acute promyelocytic leukemia with tretinoin (all-trans-retinoic acid). N Engl J Med 324: 1385–1393
Yang X, Ferguson AT, Nass SJ, Phillips DL, Butash KA, Wang SM, Herman JG, Davidson NE (2000) Transcriptional activation of estrogen receptor α in human breast cancer cells by histone deacetylase inhibition. Cancer Res 60: 6890–6894
This work was supported by grants from MIUR (PRIN 2002, No. MM06103241) and the University of Florence (ex 60%). AP was a fellow of AIL (Associazione Italiana contro le Leucemie; Firenze).
Rights and permissions
From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
About this article
Cite this article
Cellai, C., Laurenzana, A., Vannucchi, A. et al. Growth inhibition and differentiation of human breast cancer cells by the PAFR antagonist WEB-2086. Br J Cancer 94, 1637–1642 (2006). https://doi.org/10.1038/sj.bjc.6603156
- breast cancer
- growth arrest
This article is cited by
Does upregulated host cell receptor expression provide a link between bacterial adhesion and chronic respiratory disease?
Journal of Translational Medicine (2016)
Platelet-activating factor receptor-mediated PI3K/AKT activation contributes to the malignant development of esophageal squamous cell carcinoma
Platelet-activating factor induces proliferation in differentiated keratinocytes
Molecular and Cellular Biochemistry (2013)
Inflammation and melanoma growth and metastasis: The role of platelet-activating factor (PAF) and its receptor
Cancer and Metastasis Reviews (2007)