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Novel IL-15 isoforms generated by alternative splicing are expressed in the intestinal epithelium

Genes & Immunity volume 7, pages 407–416 (2006)Cite this article

Abstract

Previous studies have identified mRNA three isoforms encoding interleukin-15 (IL-15) that are produced through differential splicing and encode for the same mature IL-15 protein with two different signal peptides. Our analysis of mouse intestinal epithelial cells revealed two new IL-15 mRNA isoforms generated by different alternative splicing events. In one form (IL-15ΔE6), exon 6 is absent, and in the second form the first 48 nt of exon 7 are absent (IL-15ΔE7) through usage of an alternative 5′ splicing site within exon 7. These mRNA isoforms encoded in-frame IL-15 protein variants lacking either 15aa (IL-15ΔE6) or 16aa (IL-15ΔE7) both utilizing the normal long signal peptide. Significant structural changes were predicted for these new IL-15 isoforms. RNAse protection assays revealed the highest expression of isoform mRNA in the intestinal epithelium and functional analysis of recombinant IL-15 isoform proteins suggested possible regulatory functions.

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  1. Department of Immunology, University of Connecticut Health Center, Farmington, CT, USA

    X Tan & L Lefrançois

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  2. L Lefrançois
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Correspondence to L Lefrançois.

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Tan, X., Lefrançois, L. Novel IL-15 isoforms generated by alternative splicing are expressed in the intestinal epithelium. Genes Immun 7, 407–416 (2006). https://doi.org/10.1038/sj.gene.6364314

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