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Interleukin-20 promotes angiogenesis in a direct and indirect manner

Genes & Immunity volume 7, pages 234–242 (2006)Cite this article

Abstract

IL-20 belongs to the IL-10 family and is involved in the pathogenesis of keratinocyte hyperproliferation in vivo. Endothelial cells express IL-20 receptors. To explore the function of IL-20 on endothelial cells, we treated human umbilical vein endothelial cells (HUVECs) and human microvascular endothelial cells (HMECs) with human IL-20 and analyzed its effect on endothelial cells. IL-20 induced proliferation of endothelial cells and the activity was specifically blocked by anti-human-IL-20 monoclonal antibody and soluble (s)IL-20 receptor (R)1 and sIL-20R2. An alternatively spliced variant of IL-20 was isolated and also was shown to induce proliferation of HUVECs and HMECs. Treatment of HUVECs with both IL-10 and IL-20 demonstrated that IL-10 antagonized the activity of IL-20 because it diminished IL-20-induced proliferation of HUVECs. IL-20 significantly induced HUVECs migration and vascular tube formation on Matrigel in vitro. In vivo, IL-20 also enhanced tumor angiogenesis. Incubation of IL-20 with HUVECs induced transcripts of bFGF, VEGF, MMP-2, MMP-9, and IL-8. Furthermore, incubation of HUVECs with IL-20 induced phosphorylation of ERK1/2, p38, and JNK. Thus, IL-20 is a pleiotropic cytokine and promotes angiogenesis.

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Acknowledgements

This study was supported by a grant from Chi-Mei Medical Center, Tainan, Taiwan.

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Authors and Affiliations

  1. Chi-Mei Medical Center, Tainan, Taiwan

    M-Y Hsieh, B-C Cheng, T-Y Huang & M-S Chang

  2. Department of Biochemistry and Molecular Biology, Medical College, National Cheng Kung University, Tainan, Taiwan

    M-Y Hsieh, W-Y Chen & M-S Chang

  3. Institute of Basic Medical Sciences and Department of Cell Biology and Anatomy, Medical College, National Cheng Kung University, Tainan, Taiwan

    W-Y Chen, M-J Jiang & B-C Cheng

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  1. M-Y Hsieh
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Correspondence to M-S Chang.

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Hsieh, MY., Chen, WY., Jiang, MJ. et al. Interleukin-20 promotes angiogenesis in a direct and indirect manner. Genes Immun 7, 234–242 (2006). https://doi.org/10.1038/sj.gene.6364291

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