Abstract
Genetic association with type 1 diabetes (T1D) has been established for two chromosomal regions: HLA DQ/DR (IDDM1) and INS VNTR (IDDM2). To identify additional genetic markers, we tested polymorphisms in regulatory regions of several cytokine and important metabolic genes. These polymorphisms exhibit functional consequences for expression and function. Functional genetic polymorphisms of proinflammatory (T-helper-1: IL-2, IL-12 and IFN-γ), anti-inflammatory (T-helper-2: IL-4, IL-6 and IL-10) and metabolic (IGF-I, VDR and INS) genes were determined in 206 Dutch simplex families with juvenile onset T1D and the results were analysed using the transmission disequilibrium test. Significantly increased transmission to T1D probands was observed for the loci IDDM1, IDDM2 and the vitamin D receptor. Although none of the other individual polymorphisms was associated with disease individually, the combination of T-helper-2 and metabolic/growth alleles IL-10*R2, IL-4*C, VDR*C and IGF-I*wt was found to be transmitted more frequently than expected (67%, Pc=0.015). We conclude that additional genetic predisposition to T1D is defined by combinations of markers (eg Th2 and metabolic) rather than by a single marker. The consequences of the increased transmission of a low Th2 expressing genotypes together with a normal Th1 profile may result in a net proinflammatory cytokine expression pattern.
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References
Kelly MA, Mijovic CH, Barnett AH . Genetics of type 1 diabetes. Best Pract Res Clin Endocrinol Metab 2001; 15: 279–291.
Tabor HK, Risch NJ, Myers RM . Opinion: candidate-gene approaches for studying complex genetic traits: practical considerations. Nat Rev Genet 2002; 3: 391–397.
Cox NJ, Wapelhorst B, Morrison VA et al. Seven regions of the genome show evidence of linkage to type 1 diabetes in a consensus analysis of 767 multiplex families. Am J Hum Genet 2001; 69: 820–830.
John S, Turner D, Donn R et al. Two novel biallelic polymorphisms in the IL-2 gene. Eur J Immunogenet 1998; 25: 419–420.
Morahan G, Huang D, Ymer SI et al. Linkage disequilibrium of a type 1 diabetes susceptibility locus with a regulatory IL12B allele. Nat Genet 2001; 27: 218–221.
Pociot F, Veijola R, Johannesen J et al. Analysis of an interferon-gamma gene (IFNG) polymorphism in Danish and Finnish insulin-dependent diabetes mellitus (IDDM) patients and control subjects. Danish Study Group of Diabetes in Childhood. J Interferon Cytokine Res 1997; 17: 87–93.
Rosenwasser LJ, Klemm DJ, Dresback JK et al. Promoter polymorphisms in the chromosome 5 gene cluster in asthma and atopy. Clin Exp Allergy 1995; 25: (Suppl 2): 74–78.
Eskdale J, Gallagher G, Verweij CL, Keijsers V, Westendorp RG, Huizinga TW . Interleukin 10 secretion in relation to human IL-10 locus haplotypes. Proc Natl Acad Sci USA 1998; 95: 9465–9470.
Jahromi MM, Millward BA, Demaine AG . A polymorphism in the promoter region of the gene for interleukin-6 is associated with susceptibility to type 1 diabetes mellitus. J Interferon Cytokine Res 2000; 20: 885–888.
Gross C, Krishnan AV, Malloy PJ, Eccleshall TR, Zhao XY, Feldman D . The vitamin D receptor gene start codon polymorphism: a functional analysis of FokI variants. J Bone Miner Res 1998; 13: 1691–1699.
Vaessen N, Heutink P, Janssen JA et al. A polymorphism in the gene for IGF-I: functional properties and risk for type 2 diabetes and myocardial infarction. Diabetes 2001; 50: 637–642.
Undlien DE, Bennett ST, Todd JA et al. Insulin gene region-encoded susceptibility to IDDM maps upstream of the insulin gene. Diabetes 1995; 44: 620–625.
Rabinovitch A, Suarez-Pinzon WL . Cytokines and their roles in pancreatic islet beta-cell destruction and insulin-dependent diabetes mellitus. Biochem Pharmacol 1998; 55: 1139–1149.
Almawi WY, Tamim H, Azar ST . Clinical review 103: T helper type 1 and 2 cytokines mediate the onset and progression of type I (insulin-dependent) diabetes. J Clin Endocrinol Metab 1999; 84: 1497–1502.
Bruining GJ . Association between infant growth before onset of juvenile type-1 diabetes and autoantibodies to IA-2. Netherlands Kolibrie study group of childhood diabetes. Lancet 2000; 356: 655–656.
Vafiadis P, Bennett ST, Todd JA et al. Insulin expression in human thymus is modulated by INS VNTR alleles at the IDDM2 locus. Nat Genet 1997; 15: 289–292.
Lindsay RS, Hanson RL, Wiedrich C, Knowler WC, Bennett PH, Baier LJ . The insulin gene variable number tandem repeat class I/III polymorphism is in linkage disequilibrium with birth weight but not type 2 diabetes in the Pima population. Diabetes 2003; 52: 187–193.
Stene LC, Magnus P, Lie RT, Sovik O, Joner G . Birth weight and childhood onset type 1 diabetes: population based cohort study. BMJ 2001; 322: 889–892.
Vaessen N, Janssen JA, Heutink P et al. Association between genetic variation in the gene for insulin-like growth factor-I and low birthweight. Lancet 2002; 359: 1036–1037.
LeRoith D, Werner H, Beitner-Johnson D, Roberts Jr CT . Molecular and cellular aspects of the insulin-like growth factor I receptor. Endocr Rev 1995; 16: 143–163.
Brocardo MG, Schillaci R, Galeano A et al. Early effects of insulin-like growth factor-1 in activated human T lymphocytes. J Leukoc Biol 2001; 70: 297–305.
Ogunkolade BW, Boucher BJ, Prahl JM et al. Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians. Diabetes 2002; 51: 2294–2300.
van Halteren AG, van Etten E, de Jong EC, Bouillon R, Roep BO, Mathieu C . Redirection of human autoreactive T-cells upon interaction with dendritic cells modulated by TX527, an analog of 1,25 dihydroxyvitamin D(3). Diabetes 2002; 51: 2119–2125.
Thomasset M . Vitamin D and the immune system. Pathol Biol 1994; 42: 163–172.
McCormack RM, Maxwell AP, Carson DJ, Patterson CC, Middleton D, Savage DA . The IL12B 3′ untranslated region DNA polymorphism is not associated with early-onset type 1 diabetes. Genes Immun 2002; 3: 433–435.
Jahromi M, Millward A, Demaine A . A CA repeat polymorphism of the IFN-gamma gene is associated with susceptibility to type 1 diabetes. J Interferon Cytokine Res 2000; 20: 187–190.
Pani MA, Knapp M, Donner H et al. Vitamin D receptor allele combinations influence genetic susceptibility to type 1 diabetes in Germans. Diabetes 2000; 49: 504–507.
Nerup J, Platz P, Andersen OO et al. HL-A antigens and diabetes mellitus. Lancet 1974; 2: 864–866.
Dudbridge F, Koeleman BP, Todd JA, Clayton DG . Unbiased application of the transmission/disequilibrium test to multilocus haplotypes. Am J Hum Genet 2000; 66: 2009–2012.
Acknowledgements
This study was supported by grants from the Dutch Diabetes Research Foundation (97.137, 2001.10.004), The Netherlands Organisation for Health Research and Development (ZonMW) and The Juvenile Diabetes Research Foundation International (JDRF) (2001.10.004). We thank Prof. Dr FJ Claas and Prof. Dr RRP de Vries for carefully reading this manuscript.
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Eerligh, P., Koeleman, B., Dudbridge, F. et al. Functional genetic polymorphisms in cytokines and metabolic genes as additional genetic markers for susceptibility to develop type 1 diabetes. Genes Immun 5, 36–40 (2004). https://doi.org/10.1038/sj.gene.6364036
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DOI: https://doi.org/10.1038/sj.gene.6364036
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