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Genomic analysis of Th1–Th2 cytokine genes in an AIDS cohort: identification of IL4 and IL10 haplotypes associated with the disease progression

Genes & Immunity volume 4, pages 441–449 (2003)Cite this article

Abstract

Polymorphisms of Th1–Th2 cytokine genes have previously been implicated in the rate of progression to AIDS in seropositive patients. To evaluate further the impact of these genes in the development of AIDS, we have performed an extensive genetic analysis of IL2, IL4, IL6, IL10, IL12p35 and p40, IL13 and IFNγ. The coding regions and promoters of these genes were sequenced in a Caucasian cohort of 337 HIV-1 seropositive extreme patients (the GRIV cohort) consisting of patients with slow progression and rapid progression, and up to 470 healthy controls. In all, 64 single nucleotide polymorphisms (SNPs) and four deleterious polymorphisms with frequency >1% were identified and evaluated for their association with disease. Statistically significant associations were observed with haplotypes of the IL4 and IL10 genes, but no relation was found with variants of other genes. The catalogue of SNP and haplotypes presented here will facilitate further genetic investigations of Th1–Th2 cytokines in AIDS and other immune-related disorders.

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Acknowledgements

We are grateful to all the patients and all the medical staff who have generously collaborated with the GRIV project. We also thank the EGEA cooperative group for having given access to data on the EGEA (Epidemiological study on the Genetics and Environment of Asthma) study, which was partly supported by an INSERM/MSD convention. The GRIV project is supported by grants from ANRS (Agence Nationale de Recherche sur le SIDA) and ACV (AIDS-Cancer Vaccine Development Foundation). The CNG is supported by the Ministere de la Recherche et des Nouvelles Technologies.

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Authors and Affiliations

  1. Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, Paris, France

    A Vasilescu, R Ivanova, H Hendel, H Do, A Mazoyer, E Khadivpour, F X Goutalier & J-F Zagury

  2. Centre National de Génotypage, 2, rue Gaston Crémieux, Évry, France

    A Vasilescu, S C Heath, R Ivanova, H Do, A Mazoyer, G M Lathrop & F Matsuda

  3. Center for Neurovirology and Cancer Biology, Temple University, Philadelphia, PA, USA

    K Khalili, J Rappaport & J-F Zagury

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  1. A Vasilescu
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Correspondence to J-F Zagury.

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Vasilescu, A., Heath, S., Ivanova, R. et al. Genomic analysis of Th1–Th2 cytokine genes in an AIDS cohort: identification of IL4 and IL10 haplotypes associated with the disease progression. Genes Immun 4, 441–449 (2003). https://doi.org/10.1038/sj.gene.6363983

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