Association between COL1A1 gene polymorphisms and bone size in Caucasians

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Abstract

Bone size is an important determinant of bone strength and a risk factor of osteoporotic fracture. Several studies indicate that bone size has a high heritability. Thus, a better understanding of genetic factors regulating bone size might have important clinical implications. In the present study, we examined the relationship between the collagen type I alpha 1 (COL1A1) gene and bone size at the spine, hip and wrist in a sample of 1873 subjects of Caucasian origin from 405 nuclear families. Three single-nucleotide polymorphisms (SNPs) in the COL1A1 gene were analyzed. The minor allele frequencies were 15.4, 18.8, and 1.9% for SNP1, SNP2, and SNP3, respectively. Haplotypes were reconstructed based on the family information as well as marker genotypes using the program Genehunter. We did not find evidence of population stratification, within-family association, or linkage for either single SNPs or haplotypes at any skeletal site. Suggestive evidence of total association was observed for the wrist size at SNP2 (P=0.011). After adjusting age, sex, height, and weight, subjects with the T allele of SNP2 had, on average, 3.05% smaller wrist size than noncarriers. When the subjects were divided into families with only female offspring and families with male offspring only, similar total associations were found at the wrist size for SNP2 with P-values of 0.011 and 0.010, respectively. In conclusion, the COL1A1 gene may have some effects on bone size variation at the wrist, but not at the spine or hip in our Caucasian nuclear families.

Introduction

Osteoporosis is one of the most common bone and mineral disorders. It is mainly characterized by low bone mineral density (BMD), which is defined as the ratio of bone mineral content to bone size.1 Hence, bone size is a component of BMD and also a simpler phenotype than BMD, in terms of the complexity of the influencing factors.2 The pathogenesis of bone fragility is heterogeneous. Low BMD is not the only important risk factor for osteoporotic fractures, but several other factors also contribute, including muscle strength, bone quality, and bone shape.3,4 Studies have suggested that bone size per se may play an important role that is independent of BMD and BMC in determining bone strength.5,6 The QTLs for BMD and bone size are different.7 A decrease in bone mass may result in a loss of bone strength, and the loss may be offset, at least in part, by an increase in bone size, tending to preserve bone strength.8 A deficit in bone size may partly account for both the increased bone fragility and the deficit in BMD relative to age-matched controls.6

Compared with BMD, genetic studies on bone size have been rather scarce so far, even though the genetic determination of bone size has been characterized.2,9 Generally, the heritability of bone size at the spine, hip, and wrist is over 50%.2 Several genomic regions and candidate genes have been shown to be linked to the variation of bone size.7,10 Collagen type I, the most abundant protein in bone, is composed of two alpha 1 chains and one alpha 2 chain, which are encoded by the corresponding genes, collagen type I alpha 1 (COL1A1) and collagen type I alpha 2 (COL1A2). Grant et al11 first described a G-T polymorphism at the first base of a binding site for the transcription factor Sp1 in the first intron of the COL1A1 gene that was associated with low bone density and increased occurrence of osteoporotic fracture. The relevance of this polymorphism to risk of fracture was also reported to be independent of BMD and mediated through variations in bone size and geometry.12,13,14

In this study, we investigated three single-nucleotide polymorphisms (SNPs) in the COL1A1 gene to analyze whether they have effects on bone size variation at the lumbar spine, hip, and wrist in a large sample of Caucasian nuclear families.

Materials and methods

Subjects

The study subjects came from an expanding database being created for studies to search for genes underlying the risk of osteoporosis and obesity at the Osteoporosis Research Center of Creighton University. The study was approved by the Creighton University institutional review board. All subjects were Caucasians of European origin. The exclusion criteria detailed by Deng et al15 were adopted to minimize any known potential confounding effects on the study phenotypes. A total of 1873 subjects from 405 nuclear families were recruited. All subjects signed informed-consent documents before entering the project. For each study subject, information on age, sex, medical history, and family history was acquired.

Measurement

As described in Deng et al,7 bone sizes of the lumbar spine (L1–L4), total hip, and wrist (mid-area of forearm) were measured by a dual energy X-ray absorptiometry (DXA) with a Hologic 2000+ or 4500 scanner (Hologic Inc., Bedford, MA, USA). Bone size was reported as area measurement in units of cm2. Both scanners were calibrated daily, and long-term precision was monitored with phantoms. Measurements from the two machines were highly comparable and were well within the precision limits.2 In addition, when treating scanner type as a covariate, its effect was not significant.2 Members of the same nuclear family were measured with the same scanner. Approximately 92% of the subjects were measured with the Hologic 4500 machine. Height and weight were measured at the same visit for bone size measurement.

Genotyping

Three SNPs in the COL1A1 gene (named SNP1–SNP3) were chosen according to the literatures11,16 and the function of the SNP, i.e., whether there is amino-acid change. DNA was extracted from whole blood using a commercial isolation kit (Gentra Systems, Minneapolis, MN, USA). The genotyping procedure for all SNPs was similar, involving polymerase chain reaction (PCR) and invader assay reaction (Third Wave Technology, Madison, WI, USA). PCR was performed in a 10 μl reaction volume with 30 cycles. The sequences of the PCR primers for all three SNPs are presented in Table 2. After amplification, an invader reaction was performed in a 7.5 μl reaction volume, with 3.75 μl diluted PCR product (1:20), 1.5 μl probe mix, 1.75 μl Cleavase FRET mix, and 0.5 μl Cleavase enzyme/MgCl2 solution (Third Wave Technology). The reaction mix was first denatured at 95C for 5 min, and then incubated at 63C for 20 min. The genotype for every sample was called according to the ratio of the fluorescence intensity of the two dyes, which was read using a Cytofluor 4000 multiwell plate reader (Applied Biosystems, Foster City, CA, USA). After genotyping, the program PedCheck (http://watson.hgen.pitt.edu/register/soft_doc.html) was employed to verify Mendelian inheritance of all the marker alleles within each family.17

Table 2 Information and the primer sequences for the studied SNPs in the COL1A1 gene

Statistical analyses

The allele frequencies of each SNP were estimated in all 1873 individuals via a maximum-likelihood method implemented in the program SOLAR (http://www.sfbr.org/sfbr/public/software/solar). Haplotypes for all 1873 individuals were reconstructed using program Genehunter version 2.1, which is available at http://www.hgmp.mrc.ac.uk/Registered/Option/genehunter.html. The SNPs were arranged according to their locations on the chromosome in the order of SNP1-SNP2-SNP3. The haplotype frequencies were computed in the unrelated sample, parents from 405 nuclear families. Pairwise linkage disequilibrium (LD) between the SNPs were calculated by the normalized measure, D′,18 and was examined by Fisher's exact test.19

Using the QTDT program (http://www.sph.umich.edu/csg/abecasis/QTDT/), we tested population stratification, linkage, and association between the SNPs and the haplotypes with bone size variation at the three skeletal sites. These tests were described by Abecasis et al20 and Fulker et al.21 In order to prevent associations based on a few individuals with rare haplotypes that may lead to spurious ‘statistical association’, QTDT tests were only conducted for the three common haplotypes (shown in the results below). Multivariate normality of the phenotypic data is a critical assumption of the variance component approach such as those implemented in the QTDT. In the present study, all of the three phenotypes were found to deviate from normal distribution (P<0.01) by performing the Anderson–Darling test, and therefore, were transformed to approach normality via the Box–Cox procedure. Both procedures were implemented in the statistical software MINITAB (Minitab Inc., State College, PA, USA). All the QTDT analyses were performed on the transformed data with age, sex, height, and weight as covariates, which generally affect bone size variation significantly.2,7

Some genes may have effects on the phenotypes either only in males or only in females, and such effects may be masked in a mixed-gender study.22 Therefore, in order to detect whether the relationship between the COL1A1 gene and bone size was modified by gender, the subjects were divided into two groups: nuclear families with daughters (female offspring families) and nuclear families with sons (male offspring families). For those families with several opposite-sex offspring, the male offspring and parents were grouped into male offspring families and the female children with the parents were used to construct female offspring families. For those parentless families, if there were at least two brothers or two sisters, they were used to construct the male offspring families or female offspring families, respectively. In this situation, phenotypes were not adjusted for sex since the parents’ phenotypes were excluded in the QTDT.

In this study, the relationship of both SNPs and haplotypes with three phenotypes were tested. These tests resulted in a multiple testing problem. Therefore, a Monte-Carlo permutation test23 was performed 1000 times to establish an empirical threshold, which was about 0.008 for an individual test to achieve a global significance level of 0.05 for our analyses in this study.

Results

Characteristics of the study subjects

In the present study, 1873 subjects from 405 nuclear families were analyzed, including 740 parents, 389 sons, and 744 daughters. The nuclear families varied in size from three to 12 individuals, with a mean of 4.62 individuals (±SD=1.78). 341 families were composed of both parents and at least one offspring. The remaining 64 families, with one or no parent, contained two or more children. There were 27.2, 22.7, 22.7 and 27.4% of nuclear families with one, two, three, and more than three children, respectively, yielding a total of 1512 sib pairs. Descriptive characteristics of the children stratified by sex are presented in Table 1.

Table 1 Basic characteristics of the daughters and sons

Frequencies of the alleles and haplotypes

Frequencies of the SNP alleles and haplotypes are presented in Tables 2 and 3, respectively. The minor allele frequencies were 15.4, 18.8, and 1.9% for SNP1, SNP2, and SNP3, respectively. Haplotypes were reconstructed for all 1873 individuals based on the pedigree information and marker genotypes. Seven haplotypes were inferred, while another theoretically possible haplotype, TTA, was missing in our sample. Three major haplotypes, TGG, GGG, and GTG, accounted for 97.8%, and the other four rare haplotypes, TGA, TTG, GGA, and GTA, only amounted to 2.2%.

Table 3 P-values of total association testsa between the COL1A1 gene and bone size

Association and/or linkage between SNPs and haplotypes with bone size

We did not find evidence of population stratification, within-family association and linkage for either single SNPs or haplotypes at any skeletal site. Even when the subjects were divided into female offspring families or male offspring families, we still did not get any evidence for these tests (data not shown). For the total association test, the P-value was 0.011 for wrist size variation at the SNP2 (Table 3). After adjusted by age, sex, height, and weight, T allele carriers had an average of 3.05% smaller wrist size than noncarriers. When the sample was divided into female offspring families or male offspring families, similar results were observed between SNP2 and wrist size with P-values of 0.011 and 0.010, respectively. The haplotype GTG was found to be weakly associated with wrist size variation in the female offspring families, the male offspring families, and total subjects (P=0.023, 0.041, and 0.026, respectively). Weak evidence of total association was observed between the haplotype GGG and spine size in the female offspring families and the total nuclear families with P-values of 0.028 and 0.022, respectively. The other unspecified results dramatically exceeded the significance level with P>0.10.

Discussion

In QTDT, within-family association test is based on the allelic transmission through the nuclear family and is robust to population stratification/admixture. However, it leads to a potentially large reduction in power to detect allelic association because only the offspring from informative families are used.24 In total association test, all offspring individuals are used, further increasing the power to detect association compared to the within-family association test. However, total association test may produce misleading results in the case of population stratification/admixture. In the present study, we did not find evidence of population stratification/admixture; therefore, the more powerful test of total association was utilized.

To date, most studies of osteoporosis have focused on the possible genetic influences on BMD.25 However, BMD is not the only determinant of skeletal fragility. Bone size and geometry also determine bone mechanical strength and predict the risk of fracture independent of BMD.26,27 A strong genetic influence on the skeletal size has been well established,2,9 but the genes and allelic variants regulating bone size are not well defined. COL1A1 is an important candidate gene for osteoporosis, which encodes the alpha 1 chain of type I collagen, the most abundant protein of bone matrix. In this study, we have set out to investigate the relationship between the COL1A1 gene polymorphisms and bone size variation at three skeletal sites in a large sample of Caucasian nuclear families. We found that SNP2, the Sp1 polymorphism in intron 1 of COL1A1, was suggestively associated with the wrist size variation in both female and male offspring families and the total subjects as well. Although SNP2 has been reported to be associated with height,28 we did not find such an association either in the total nuclear families or the female or male offspring families. When height was excluded from the covariates, the P-values for the total association between SNP2 with wrist size increased from 0.011, 0.010, and 0.011 (when height was used as a covariate) to 0.059, 0.042, and 0.020 in the female offspring families, male offspring families, and total nuclear families, respectively. However, for the other skeletal sites and the other SNPs and haplotypes, the results were similar whether height was used as a covariate or not.

Functional studies have shown that SNP2 alters binding of Sp1 to its recognition site and is associated with disturbances in COL1A1 transcription, collagen protein production, and the biomechanical properties of bone.29 The T allele of this SNP was reported to act as a risk factor to osteoporotic fracture in some studies,12,14 but not in other populations.30,31 We also analyzed the relationship between SNP2 and BMD in this population, and the risk effect of this SNP was not observed (Long J-R, personal communication). So far, only a few studies have been conducted to address the relationship between this SNP and bone geometry or bone structure. Qureshi et al32 found that in British women, femoral neck-shaft angle was increased by 2° in the TC/TT genotype groups compared with CC homozygotes. However, they did not find any significant difference in hip axis length or femoral neck width between different genotype groups. This result was similar to ours in that no significant association was observed between SNP2 and hip size. Our results of no association between SNP2 and spine size were consistent with the findings of Sainz et al,33 in which there was no significant difference between different genotypes in the size of the vertebrae. However, in another study,28 the T allele carriers were reported to have 0.5 SD smaller width of the lumbar vertebrae. The differences between studies in design, measurement of bone size, ethnic background, and skeletal sites make it difficult to establish concordance.34

Our suggestive significant association between SNP2 with wrist size was compatible with the results of whole genome scan performed by our group. In a sample of 53 pedigrees with 630 subjects,7 a QTL for wrist bone size variation with a two-point LOD=3.98, 75 cM from pter, and multipoint LOD=3.01, 77 cM from pter (Figure 1) was observed on chromosome 17q23, a region quite close to 17q21.31–q22 where COL1A1 is located, 82 cM from pter. However, one issue that should be mentioned is that there are some other candidate genes located in this region, such as T-BOX 2 (83 cM from pter) and Noggin (78 cM from pter). In this study, we did not find the evidence of linkage for the COL1A1 gene to the wrist size. One of the possible reasons is that the linkage approach is of limited statistical power in detecting and/or confirming QTLs with currently employed sample sizes.35,36,37,38 Power calculations (http://statgen.iop.kcl.ac.uk/gpc/) showed that we only had 12% power to detect linkage to a gene accounting for 2% of phenotypic variation with a recombination fraction of 0.05 and a false-positive error rate of 0.05. While the power to detect association for this study was 64%, assuming that a QTL explained 2% of the phenotypic variation and the LD between the studied marker and QTL is D′=0.90. However, the power reduced dramatically if the marker and QTL were not in strong LD.

Figure 1
figure1

Maximum multipoint LOD score for wrist bone size on the chromosome 17 from Deng et al7 and the location of the COL1A1 gene in this region.

There are several potential limitations in this study that should be acknowledged. First, DXA has been accepted as the most commonly used technique to assess bone phenotypes in epidemiological studies. It provides an intense, narrow beam of radiation, shortened scan time, enhanced image definition, improved precision, and a relative stability of calibration.39 However, it provides a two-dimensional approximation of a three-dimensional structure of bone, which can be measured by volumetric quantitative computed tomography and magnetic resonance imaging.40 However, these two methods are not readily available for large-scale studies due to the high cost, long analysis time, and a relatively high radiation exposure. Second, the children in our sample have a relatively large range of age (19–75 years); which might affect the studied phenotypes. Moreover, the total length of the COL1A1 gene was 18 kb and we only examined three SNPs, there being a 12.2 kb gap between SNP2 and SNP3. This may be the reason that we did not get evidence in haplotype analysis, although some suggestive association between SNP2 and wrist size was observed.

In summary, we have examined the association and/or linkage between the COL1A1 gene and bone size at the spine, hip, and wrist in a sample of Caucasian population. We did not find any linkage of this gene to bone size variation at any of the studied skeletal sites, while, suggestive evidence of association was observed at SNP2 for the wrist but not the spine or hip.

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Acknowledgements

The study was partially supported by grants from Health Future Foundation of the USA, grants from the National Institute of Health (K01 AR02170-01, R01 GM60402-01 A1, P01 DC01813-07), grants from the State of Nebraska Cancer and Smoking Related Disease Research Program (LB595) and the State of Nebraska Tobacco Settlement Fund (LB692), and a US Department of Energy grant (DE-FG03-00ER63000/A00).

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Correspondence to Hong-Wen Deng.

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Keywords

  • COL1A1 gene
  • bone size
  • QTDT

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