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Extracellular matrix degrading enzymes at the prostasome surface

Prostate Cancer and Prostatic Diseases volume 8, pages 344–348 (2005)Cite this article

Abstract

Prostasomes, prostatic secretory vesicles found in human ejaculates, were analyzed to verify the existence at their surfaces of enzymes involved in the degradation of the extracellular matrix. Findings were compared with those of prostasomes isolated from two human adenocarcinoma cell lines that reflect clinical features and molecular pathways of androgen-insensitive and hormone-responsive prostate cancer. Our aim was to determine whether neoplastic transformation is accompanied by changes of glycosidase and protease activities. Our results show that decreases of dipeptidyl peptidase IV and increases of urokinase plasminogen activator and cathepsin B are consistent with the clinical features of the cell lines, whereas increases of glycosidase activities seem to be of scarce biological significance.

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Acknowledgements

We thank Fondazione Cassa di Risparmio di Perugia for funding the research. We are grateful to Dr M Kerrigan (MA, Cantab) for helpful linguistic suggestions.

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Authors and Affiliations

  1. Dipartimento Medicina Sperimentale Scienze Biochimiche, Sezione Biochimica Cellulare, Perugia, Italy

    I Bellezza, R Palazzo, E Costanzi & A Minelli

  2. Dipartimento Medicina Interna, Sezione Biochimica Applicata Scienze Nutrizionali, Perugia, Italy

    M C Aisa

  3. Diparimento Specialità Chirurgiche, Sezione di Urologia, Università di Perugia, Perugia, Italia

    E Mearini

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  1. I Bellezza
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  2. M C Aisa
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  3. R Palazzo
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  4. E Costanzi
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  5. E Mearini
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  6. A Minelli
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Correspondence to A Minelli.

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Bellezza, I., Aisa, M., Palazzo, R. et al. Extracellular matrix degrading enzymes at the prostasome surface. Prostate Cancer Prostatic Dis 8, 344–348 (2005). https://doi.org/10.1038/sj.pcan.4500828

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  • DOI: https://doi.org/10.1038/sj.pcan.4500828

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