Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Paper
  • Published:

Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections

Prostate Cancer and Prostatic Diseases volume 2, pages 140–147 (1999)Cite this article

Abstract

We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1-antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections of ten benign and six malignant prostate tissue specimens. The signal intensity increased linearly with the amount of labelled Mab until epitope saturation began. The highest concentrations of bound IgG in tissue sections were 27.3 fmol/pixel for ACT and 7.2 for PSA. Time-resolved fluorescence imaging (TRFI) offers an attractive method for histochemical studies based on specific and quantitative detection of fluorescent lanthanide chelates.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Urology, University Hospital, Malmö, Lund University, Sweden

    A Bjartell

  2. Department of Clinical Chemistry, University Hospital, Malmö, Lund University, Sweden

    H Lilja

  3. Department of Biotechnology, University of Turku, Finland

    P Siivola, S Hulkko, K Pettersson & T Lövgren

  4. Wallac OY, Turku, Finland

    K Rundt

Authors
  1. A Bjartell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P Siivola
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S Hulkko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K Pettersson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K Rundt
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H Lilja
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T Lövgren
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bjartell, A., Siivola, P., Hulkko, S. et al. Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections. Prostate Cancer Prostatic Dis 2, 140–147 (1999). https://doi.org/10.1038/sj.pcan.4500307

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.pcan.4500307

Keywords

Search

Advanced search

Quick links