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Gene therapy progress and prospects: Hydrodynamic gene delivery

Gene Therapy volume 14, pages 99–107 (2007)Cite this article

Abstract

Over the last few years, hydrodynamic tail vein delivery has established itself as a simple, yet very effective method for gene transfer into small rodents. Hydrodynamic delivery of plasmid DNA expression vectors or small interfering RNA allows for a broad range of in vivo experiments, including the testing of regulatory elements, antibody generation, evaluation of gene therapy approaches, basic biology and disease model creation (non-heritable transgenics). The recent development of the hydrodynamic limb vein procedure provides a safe nucleic acid delivery technique with equally high efficiency in small and large research animals and, importantly, the prospects for clinical translation.

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  1. Mirus Bio Corporation, Madison, WI, USA

    H Herweijer & J A Wolff

  2. Departments of Pediatrics and Medical Genetics, Waisman Center, University of Wisconsin-Madison, Madison, WI, USA

    J A Wolff

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Herweijer, H., Wolff, J. Gene therapy progress and prospects: Hydrodynamic gene delivery. Gene Ther 14, 99–107 (2007). https://doi.org/10.1038/sj.gt.3302891

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