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Cellular factors influencing Semliki Forest Virus vector biology

Gene Therapy volume 12, pages S111–S117 (2005)Cite this article

Abstract

Viral vectors are currently the best tools for gene delivery in a therapeutic setting, especially for in vivo use. Alphaviruses, a family of positive singlestranded RNA viruses, have been engineered to allow the formation of a highly efficient replicon. Using these replicons, it is possible to generate recombinant particles. Parental viruses and recombinant vectors share certain pathways while interacting with their target cells. In this review, we describe the consecutive events leading to transduction, and transgene expression, in view of the cellular factors that affect each individual step. Classical virology will benefit from the knowledge accumulated studying vectors, and such work will shed light on crosstalk between intruding viruses and their hosts. Ultimately, these data should help the design of vectors adapted to specific target cells.

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Acknowledgements

We thank GJ Towers for careful reading of the manuscript and the AFM and VLM for their financial support.

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Authors and Affiliations

  1. Université François Rabelais, The Vector Group, Faculté de Médecine, Tours, France

    E Piver, A Diatta, P Vaudin & J-C Pagès

  2. Biochemistry, Trousseau University Hospital, Tours, France

    C Collin

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Piver, E., Collin, C., Diatta, A. et al. Cellular factors influencing Semliki Forest Virus vector biology. Gene Ther 12 (Suppl 1), S111–S117 (2005). https://doi.org/10.1038/sj.gt.3302625

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