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Prevention and regression of atopic dermatitis by ointment containing NF-kB decoy oligodeoxynucleotides in NC/Nga atopic mouse model

Gene Therapy volume 9, pages 1221–1229 (2002)Cite this article

Abstract

Atopic dermatitis, a chronic inflammatory skin disease characterized by relapsing eczema and intense prurigo, requires effective and safe pharmacological therapy. In this study, we examined the efficacy of ointment containing NF-kB decoy oligodeoxynucleotides (ODN) on atopic dermatitis lesions in NC/Nga mice, which are characterized by the spontaneous onset of atopic dermatitis in conventional conditions. Topical administration of NF-kB decoy ODN twice a month resulted in a significant reduction in clinical skin condition score and marked improvement of histological findings. Reduction of the atopic skin condition by NF-kB decoy ODN was accompanied by a significant decrease in migration of mast cells into the dermis and an increase in apoptotic cells. Here, we demonstrated the successful treatment of atopic dermatitis with ointment containing NF-kB decoy ODN in a mouse model, promising new therapy for atopic dermatitis.

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Acknowledgements

We thank Mr T Ouchi (SRL) for histological examination, Mr Y Tatsumi (Nara Pathological Research Center) for immunohistochemical examination, Dr M Matsumoto and Dr M Kotani (SunStar) for helpful advice on the clinical skin condition score of atopic dermatitis. This work was partially supported by a grant from the Japan Health Sciences Foundation, a Grant-in-Aid from The Ministry of Public Health and Welfare, a Grant-in-Aid for the Development of Innovative Technology, a Grant-in-Aid from Japan Promotion of Science, and Special Coordination Funds of the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government.

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Authors and Affiliations

  1. Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan

    H Nakamura, Y Kaneda & R Morishita

  2. Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Japan

    M Aoki, T Ogihara & R Morishita

  3. Department of Dermatology, Hirosaki University Medical School, Hirosaki, Japan

    K Tamai

  4. Department of Pharmacy, Osaka University Hospital, Suita, Japan

    M Oishi

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  1. H Nakamura
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  2. M Aoki
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  4. M Oishi
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  7. R Morishita
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Nakamura, H., Aoki, M., Tamai, K. et al. Prevention and regression of atopic dermatitis by ointment containing NF-kB decoy oligodeoxynucleotides in NC/Nga atopic mouse model. Gene Ther 9, 1221–1229 (2002). https://doi.org/10.1038/sj.gt.3301724

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