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  • Viral Transfer Technology
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Production of first generation adenovirus vectors: a review

Gene Therapy volume 7, pages 1707–1714 (2000)Cite this article

Abstract

In the past decade, adenovirus vectors have generated tremendous interest, especially in gene therapy applications. In the so-called ‘first generation’ adenovirus vectors, the transgenes are inserted in place of the E1 region, or less often the E3 region. Although second-generation and helper-dependent adenovirus vectors will probably prevail in the future in applications that require long-term gene expression, first generation adenovirus vectors will remain very useful in other settings, such as cancer and vaccination, or simply to transfect cell lines that are refractory to other transfection methods. Until a few years ago, the construction of first generation adenovirus vectors was a labor-intensive and time-consuming process. More than 20 methods have appeared that facilitate their construction and are reviewed below.

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Acknowledgements

MJI acknowledges the support of NIH grants GM34902 and HL64762. XD is the recipient of a fellowship from the Mountain States Medical Research Institute.

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  1. O.D. 260 Inc, Mountain States Medical Research Institute, and VA Medical Center, Boise, ID, USA

    X Danthinne

  2. Department of Microbiology and Immunology and Center for Gene Therapy, University of Michigan Medical School, Ann Arbor, MI, USA

    M J Imperiale

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Danthinne, X., Imperiale, M. Production of first generation adenovirus vectors: a review. Gene Ther 7, 1707–1714 (2000). https://doi.org/10.1038/sj.gt.3301301

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