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  • Acquired Diseases
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Soluble Flt-1 gene therapy for peritoneal metastases using HVJ-cationic liposomes

Gene Therapy volume 7, pages 1027–1033 (2000)Cite this article

Abstract

Many studies have reported a close association between VEGF and tumor angiogenesis. The aim of the present study was to evaluate the effectiveness of gene therapy against cancer, including peritoneal metastasis, using a cDNA encoding a soluble type of Flt-1, one of the VEGF receptors. In a peritoneal metastasis model of MKN45 human gastric cancer cells, mice repetitively treated with intraperitoneal injections of HVJ-Fex, a type of HVJ-cationic liposome encapsulating a plasmid expressing soluble mFlt-1, exhibited smaller disseminated foci with fewer microvessels, thus resulting in a significantly longer survival period than the control mice. In another peritoneal metastasis model using HT1080S cells, a clone of HT1080 human fibrosarcoma cells stably transfected with hVEGF, treatments with HVJ-Fex also reduced the growth of disseminated foci without ascites formation. In conclusion, this study demonstrated that the peritoneal metastases of some cancers were largely dependent on VEGF, and that the repeated intraperitoneal transduction of a soluble flt-1 gene using HVJ-cationic liposomes suppressed peritoneal metastases, thereby contributing to a longer survival period.

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Acknowledgements

We thank Dr G Breier from the Max Planck Institute for Physiological and Clinical Research for providing us with the plasmid containing the full length murine flt-1 cDNA.

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Authors and Affiliations

  1. Department of Surgery and Surgical Basic Science, Department of Internal Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    A Mori, S Arii, M Furutani, M Mizumoto, S Uchida, H Furuyama, MJ Gorrin-Rivas, K Furumoto & M Imamura

  2. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    Y Kondo

  3. Division of Gene Therapy Science, Osaka University School of Medicine, Suita, Osaka, Japan

    Y Kaneda

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Mori, A., Arii, S., Furutani, M. et al. Soluble Flt-1 gene therapy for peritoneal metastases using HVJ-cationic liposomes. Gene Ther 7, 1027–1033 (2000). https://doi.org/10.1038/sj.gt.3301202

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