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Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Gene Therapy volume 6, pages 198–208 (1999)Cite this article

Abstract

We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and trans- lation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049, Madrid, Spain

    P de Felipe, V Martín, M L Cortés & M Izquierdo

  2. Department of Biochemistry, University of St Andrews, St Andrews, Fife, UK

    M Ryan

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  1. P de Felipe
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  2. V Martín
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  3. M L Cortés
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  4. M Ryan
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  5. M Izquierdo
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de Felipe, P., Martín, V., Cortés, M. et al. Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy. Gene Ther 6, 198–208 (1999). https://doi.org/10.1038/sj.gt.3300811

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  • DOI: https://doi.org/10.1038/sj.gt.3300811

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