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IgH DJ rearrangements within T-ALL correlate with cCD79a expression, an immature/TCRγδ phenotype and absence of IL7Rα/CD127 expression

Leukemia volume 18, pages 1997–2001 (2004)Cite this article

Abstract

cCD79a and IgH VDJ/DJ rearrangements are considered to be relatively specific for B lymphoid precursors. We looked for both in cCD3+, CD7+, CD19- T-ALLs classified by TCR status into αβ or γδ/immature (IM) lineages, with individualization of HOX11L2+ T-ALLs since they represent an intermediate αβ/γδ category. cCD79a was expressed at low levels in 47% of T-ALL and was most frequent in IMγ T-ALLs. IgH rearrangements were common in γδ/IM (45%) and HOX11L2+ (35%) T-ALLs compared to HOX11L2-negative cases (3%; P<0.001). CD127 (IL7Rα) expression was also more common in the γδ/IM lineage but its expression was virtually mutually exclusive of IgH rearrangement. Low-level cCD79a expression alone should therefore not be interpreted as evidence of B lineage affiliation in immature leukemias. γδ/IM lineage T-ALLs potentially include two distinct categories: predominantly IgH+, cCD79a+, CD127- cases which retain γδ and B lymphoid potential and IgH−, cCD79a−, CD127+ cases with restricted T lineage potential.

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Authors and Affiliations

  1. Biological Hematology, Assistance Publique, Hopitaux de Paris (AP-HP), Necker-Enfants-Malades, France

    V Asnafi, K Beldjord, C Millien, M Bahloul, P LeTutour, F Valensi & E Macintyre

  2. Biological Hematology, Assistance Publique, Hopitaux de Paris (AP-HP), Trousseau, Paris, France

    K Beldjord & L Douay

  3. Biological Hematology, Assistance Publique, Hopitaux de Paris (AP-HP), CHU Nantes, France

    R Garand

  4. Biological Hematology, Assistance Publique, Hopitaux de Paris (AP-HP), INSERM EMIU210 and Université Paris V, France

    V Asnafi, C Millien, F Valensi & E Macintyre

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  1. V Asnafi
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  2. K Beldjord
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  4. C Millien
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Correspondence to E Macintyre.

Additional information

Financial support: This work was supported by the fondation contre la leucémie de la Fondation de France, l'Association de la Recherche sur le Cancer (ARC), the League Contre le Cancer, the Direction de Recherche Clinique de l'Assistance Publique-Hôpitaux de Paris (PHRC 97-106) and the Biomed-2 BMH4-CT98-3936 concerted action.

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Asnafi, V., Beldjord, K., Garand, R. et al. IgH DJ rearrangements within T-ALL correlate with cCD79a expression, an immature/TCRγδ phenotype and absence of IL7Rα/CD127 expression. Leukemia 18, 1997–2001 (2004). https://doi.org/10.1038/sj.leu.2403531

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