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Retention and defective assembly of the B-cell receptor in the endoplasmic reticulum of chronic lymphocytic leukaemia B cells cannot be reverted upon CD40 ligand stimulation

Leukemia volume 17, pages 1196–1199 (2003)Cite this article

TO THE EDITOR

Dramatic low expression of the B-cell receptor (BCR), composed of surface immunoglobulin associated with CD79a/CD79b, is characteristic of chronic lymphocytic leukaemia (CLL) B cells.1 It results in inability to respond to BCR ligation, a state found in anergic B cells.2,3 Despite normal transcription and intracellular synthesis, we previously showed4 that this low expression is accounted for by a defective assembly of the BCR chains resulting in the presence of unprocessed μ chains. To gain insight into the mechanism accounting for this low expression, we aimed to define the cell compartment where the BCR retention occurs. In addition, as the lack of costimulatory molecules characteristic of CLL B cells can be at least partially restored upon CD40 ligand (CD40L) stimulation,5 we investigate the effect of such stimulation on the defective IgM and CD79b intracellular assembly and surface expression.

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Acknowledgements

This work was supported by grants from Fondation de France contre la Leucémie and Association pour la Recherche sur le Cancer (ARC). We thank Pascal Roux and Emmanuelle Perret for help with confocal microscopy analysis and Reine Bouyssié for secretarial assistance. CD40L was generously provided by Amgen Corporation (Seattle, WA, USA). The confocal microscope was purchased with a donation from Marcel and Lilianne Pollack.

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  1. Unité d'Immuno-Hématologie et d'Immunopathologie, Institut Pasteur, Paris, France

    B Payelle-Brogard, C Magnac, P Oppezzo, G Dumas, G Dighiero & F Vuillier

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  1. B Payelle-Brogard
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  2. C Magnac
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Payelle-Brogard, B., Magnac, C., Oppezzo, P. et al. Retention and defective assembly of the B-cell receptor in the endoplasmic reticulum of chronic lymphocytic leukaemia B cells cannot be reverted upon CD40 ligand stimulation. Leukemia 17, 1196–1199 (2003). https://doi.org/10.1038/sj.leu.2402944

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