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CD38 expression by hematopoietic stem cells of newborn and juvenile mice

Leukemia volume 17, pages 171–174 (2003)Cite this article

Abstract

While investigators in a number of laboratories have documented that the hematopoietic stem cells (HSCs) of fetal and adult mice are CD38+, no information is available about CD38 expression by HSCs of newborn and juvenile mice. We used a murine transplantation model to examine HSC CD38 expression. First, we observed that all HSCs from newborn bone marrow are CD38−. Next, it was determined that the majority of HSCs in the bone marrow of 5-week-old mice are CD38−, with a minority being CD38+. These observations indicated that the CD38+ subpopulation of HSC appears before the age of 5 weeks and expands during adolescence. However, the majority of HSCs of 5-week-old mice became CD38+ following injection of 5-fluorouracil, indicating that activation of juvenile stem cells enhances CD38 expression. These observations may have implications for CD38 expression by HSCs from human umbilical cord blood and bone marrow of young children in steady state and under pathological conditions.

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Acknowledgements

This work was supported by NIH grants RO1-DK54197 and PO1-CA78582 and by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs. The authors thank Anne G Livingston, Karen A Rivers and Dr Tsukasa Higuchi for assistance in preparation of this manuscript, and the staff of Radiation Oncology Department of the Medical University of South Carolina for assistance in irradiation of the mice.

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  1. Department of Veterans Affairs Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC, USA

    Y Higuchi, H Zeng & M Ogawa

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  1. Y Higuchi
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  2. H Zeng
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  3. M Ogawa
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Higuchi, Y., Zeng, H. & Ogawa, M. CD38 expression by hematopoietic stem cells of newborn and juvenile mice. Leukemia 17, 171–174 (2003). https://doi.org/10.1038/sj.leu.2402785

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