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Detection of illegitimate rearrangements within the immunoglobulin light chain loci in B cell malignancies using end-sequenced probes

Leukemia volume 16, pages 2156–2158 (2002)Cite this article

We have developed a method for detection of illegitimate rearrangements in immunoglobulin light chain loci in B cell malignancies. The assay is based on dual-colour interphase FISH, using probe sets selected within the variable and constant regions of both IGK and IGL loci resulting in split of signals upon translocations in these loci. The assay can detect: (1) translocations involving the IGK(V-J) recombination cluster; (2) translocations involving the IGL(V-J) recombination cluster; and (3) deletions and duplications.

Patient or cell line samples are prepared using conventional procedure and stored at −20°C.

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References

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Authors and Affiliations

  1. Department of Haematology L, The Research Laboratory, 54P4, Herlev Hospital, University of Copenhagen, Herlev, Denmark

    TS Poulsen

  2. Department of Medical Genetics, Wilhelm Johannsen Centre for Functional Genome Research, IMBG, The Panum Institute, Building 24.4, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark

    AN Silahtaroglu

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  1. TS Poulsen
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  2. AN Silahtaroglu
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Correspondence to TS Poulsen or AN Silahtaroglu.

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Poulsen, T., Silahtaroglu, A. Detection of illegitimate rearrangements within the immunoglobulin light chain loci in B cell malignancies using end-sequenced probes. Leukemia 16, 2156–2158 (2002). https://doi.org/10.1038/sj.leu.2402658

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  • DOI: https://doi.org/10.1038/sj.leu.2402658

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