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Chronic Lymphocytic Leukemia

Distinct gene expression profiling in chronic lymphocytic leukemia with 11q23 deletion

Leukemia volume 15, pages 1721–1728 (2001)Cite this article

Abstract

Chronic lymphocytic leukemia (CLL) is a heterogeneous disease with regard to its clinical course. The limitations of the methods currently available for prognostic assessment in CLL do not allow accurate prediction of the risk of disease progression in individual patients. The recently developed cDNA array technique provides a unique opportunity to study gene expression in various malignancies. To identify new molecular markers for prognostication of CLL patients, we analyzed cDNA arrays by using hierarchical clustering and standard statistic t-test on 34 CLL patients. We found significant expression differences in 78 genes compared to the reference tonsillar B lymphocytes. A cluster of genes, LCP1, PARP, BLR1, DEK, NPM, MCL1, SLP76, STAM, HIVEP1, EVI2B, CD25, HTLF, HIVEP2, BCL2, MNDA,PBX3, EBI2, TCF1, CGRP, CD14, IL8,GZMK, GPR17 and CD79B, was associated (P < 0.05) with the unfavorable 11q deletion and also with the unfavorable Binet stages B and C. We present here gene expression profiling that is associated with CLL patients with the 11q23 deletion. Many of the genes in the cluster have not previously been shown to be related to the initiation or progression of CLL. These novel findings provide fundamental information for further attempts to understand the interaction of the clustered genes in the leukomogenesis of CLL in order to better design treatments aimed at specific molecular target(s).

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Acknowledgements

This work was supported by grants from the Medical Research Fund of Tampere University Hospital, the Finnish Foundation for Cancer Research, the Sigrid Jusélius Foundation, Paulo Foundation, Finnish Cultural Foundation and Helsinki University Central Hospital Research Funds. We thank the Tampere CLL Group for collaboration, and Leena Pankko and Merja Suoranta for their skilful technical assistance.

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Authors and Affiliations

  1. Department of Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland

    Y Aalto, W El-Rifai, B Nagy & S Knuutila

  2. Department of Medicine, University of Virginia Health Systems, VA, USA

    W El-Rifai

  3. Department of Clinical Chemistry, Tampere University Hospital and University of Tampere, Tampere, Finland

    L Vilpo & J Vilpo

  4. Department of Biosciences, Division of Biochemistry, University of Helsinki, Helsinki, Finland

    J Ollila

  5. Genetic Laboratory, Faculty of Medicine, Semmelweis University, Budapest, Hungary

    B Nagy

  6. Department of Bioinformatics, Institute of Medical Technology, University of Tampere, Tampere, Finland

    M Vihinen

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Aalto, Y., El-Rifai, W., Vilpo, L. et al. Distinct gene expression profiling in chronic lymphocytic leukemia with 11q23 deletion. Leukemia 15, 1721–1728 (2001). https://doi.org/10.1038/sj.leu.2402282

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