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Immunobiology

MAGE-A genes are not expressed in human leukemias

Leukemia volume 15, pages 1769–1771 (2001)Cite this article

Abstract

Immunotherapy is promising to improve the prognosis of human leukemias, at least as adjuvant treatment. Tumor-associated antigens such as antigens encoded by MAGE-A1, -A2, -A3, -A4, -A6 and -A12 genes might provide tools in this field. We demonstrated recently that the presentation peptides encoded by MAGE-Agenes might make leukemic blasts suitable targets to cytolytic T lymphocytes. We reported previously negative data of MAGE-A1 gene expression in hematological malignancies, but in further studies positive results of MAGE-A gene expression were published in some subtypes of hematological malignancies such as T leukemia, myeloma and Hodgkin's disease. This led us to enlarge the screening of MAGE-A gene expression in human leukemias. In the RT-PCR screening of a large panel including 154 patients, only weak signal were detected in a few samples. We conclude that MAGE-A genes are not expressed in human leukemias.

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References

  1. Van den Eynde B, Van der Bruggen PT . Cell defined tumor antigens Curr Opin Immunol 1997 9: 684–693

    Article  CAS  Google Scholar 

  2. Van der Bruggen P, Traversari C, Chomez P, Lurquin C, De Plaen E, Van den Eynde B, Knuth A, Boon T . A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma Science 1991 254: 1643–1647

    Article  CAS  Google Scholar 

  3. Boon T . Human tumor antigens recognized by T lymphocytes J Exp Med 1996 183: 725–729

    Article  CAS  Google Scholar 

  4. De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, Van der Bruggen P, Lethé B, Lurquin C, Boon T . Structure, chromosomal localization and expression of 12 genes of the MAGE family Immunogenetics 1994 40: 360–369

    Article  CAS  Google Scholar 

  5. Chambost H, Brasseur F, Coulie P, De Plaen E, Stoppa AM, Baume D, Mannoni P, Boon T, Maraninchi D, Olive D . A tumour-associated antigen expression in human haematological malignancies Br J Haematol 1993 84: 524–526

    Article  CAS  Google Scholar 

  6. Shichijo S, Tsunosue R, Masuoka K, Natori H, Tamai M, Miyajima J, Sagawa K, Itoh K . Expression of the MAGE gene family in human lymphocytic leukaemia Cancer Immunol Immunother 1995 41: 95–103

    Article  CAS  Google Scholar 

  7. Shichijo S, Sagawa K, Brasseur F, Boon T, Itoh K . MAGE-1 gene is expressed in T-cell leukemia Int J Cancer 1996 65: 709–710

    Article  CAS  Google Scholar 

  8. Van Baren N, Brasseur F, Godelaine D, Hames G, Ferrant A, Lehmann F, André M, Ravoet C, Doyen C, Spagnoli GC, Bakus M, Tielemans K, Boon T . Genes encoding tumor-specific antigens are expressed in human myeloma cells Blood 1999 94: 1156–1164

    CAS  PubMed  Google Scholar 

  9. Chambost H, Van Baren N, Brasseur F, Godelaine D, Xerri L, Just Landi S, Theate I, Plumas J, Spagnoli GC, Michel G, Coulie P, Olive D . Expression of gene MAGE-A4 in Reed–Sternberg cells Blood 2000 95: 3530–3533

    CAS  PubMed  Google Scholar 

  10. Chambost H, Colette Y, Dutartre H, Thuret I, Olive D . Parameters involved in the recognition of fresh human leukemic blasts by tumor specific cytolytic T cells clones: a modele study Leuk Res 2000 24: 823–830

    Article  CAS  Google Scholar 

  11. Van Baren N, Chambost H, Ferrant A, Michaux L, Ikeda H, Millard I, Olive D, Boon T, Coulie PG . PRAME, a gene encoding an antigen recognized on a human melanoma by cytolytic T cells, is expressed in acute leukaemia cells Br J Haematol 1998 102: 1376–1379

    Article  CAS  Google Scholar 

  12. Brasseur F, Rimoldi R, Lienard D, Lethe B, Carrel S, Arienti F, Suter L, Vanwicjck R, Bourlond A, Humblet Y, Marchand M . Expression of MAGE genes in primary and metastatic cutaneous melanoma Int J Cancer 1995 63: 375–380

    Article  CAS  Google Scholar 

  13. Greiner J, Ringhoffer M, Simikopinko O, Szamaragowska A, Huebsch S, Maurer U, Bergmann L, Schmitt M . Simultaneous expression of different immunogenic antigens in acute myeloid leukemia Exp Hematol 2000 28: 1413–1422

    Article  CAS  Google Scholar 

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Acknowledgements

We are particularly grateful to Mrs M Swinarska and to Mrs S Landi for excellent technical assistance. We thank Pr AM Capodano and Dr C Brunet for cryopreservation of leukemic cells from childhood leukemias.

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Authors and Affiliations

  1. INSERM U119 Institut Paoli Calmettes, Marseille, France

    H Chambost & D Olive

  2. Ludwig Institute for Cancer Research, Brussels, Belgium

    N van Baren & F Brasseur

  3. Service de Pédiatrie et d'Hématologie Pédiatrique, CHU Timone, Marseille, France

    H Chambost

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  1. H Chambost
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  2. N van Baren
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  3. F Brasseur
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  4. D Olive
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Chambost, H., van Baren, N., Brasseur, F. et al. MAGE-A genes are not expressed in human leukemias. Leukemia 15, 1769–1771 (2001). https://doi.org/10.1038/sj.leu.2402278

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