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Involvement of the MLL and RARα genes in a patient with acute monocytic leukemia with t(11;17)(q23;q12)

Leukemia volume 14, pages 520–522 (2000)Cite this article

A 39-year-old white man with a history of peptic ulcer disease presented with a 1 week history of malaise, anorexia, nausea, vomiting, fevers and progressive dyspnea. He had no history of exposure to toxins or radiation. He was afebrile, without hepatomegaly, splenomegaly, lymphadenopathy, nor gum hypertrophy. Radiogram showed bilateral pulmonary infiltrates. Initial hemoglobin was 11.1 gm/dl, platelet count 31 000/mm3, white blood count 74 000/mm3. The differential showed 11% neutrophils, 4% bands, 10% lymphocytes, 4% monocytes, 64% blasts, 1% eosinophils, 4% metamyelocytes, and 2% myelocytes.

Fluorescence in situ hybridization (FISH) was performed with probes for MLL and RARα. Because of the lack of commercial availability of a separate RARα genomic probe, FISH was performed in two stages. First, hybridization was performed with probes for t(15;17) (PML orange/RARα green (Vysis, Downers Grove, IL, USA)) to verify that the PML gene on 15q22 was not translocated in this patient. No PML-RARα rearrangement was detected in 400 interphase nuclei nor 10 metaphase cells analyzed.

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Authors and Affiliations

  1. Department of Human Genetics, University of Pittsburgh, Pittsburgh, PA, USA

    S Shekhter-Levin & SM Gollin

  2. Clinical Cytogenetics Laboratory, University of Pittsburgh, Pittsburgh, PA, USA

    S Shekhter-Levin & SM Gollin

  3. Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA

    S Shekhter-Levin, SM Gollin & SS Kaplan

  4. University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA

    S Shekhter-Levin, SM Gollin, SS Kaplan & RL Redner

  5. Division of Hematology/Oncology, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA

    RL Redner

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Shekhter-Levin, S., Gollin, S., Kaplan, S. et al. Involvement of the MLL and RARα genes in a patient with acute monocytic leukemia with t(11;17)(q23;q12). Leukemia 14, 520–522 (2000). https://doi.org/10.1038/sj.leu.2401707

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