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  • Biotechnical Methods Section BTS
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Biotechnical Methods Section (BTS)

A method for identifying differentially expressed genes in rare populations of primary human hematopoietic cells

Leukemia volume 13, pages 295–297 (1999)Cite this article

Abstract

Differential display (DD) is widely employed for identifying uniquely expressed genes within two different cell populations. While potentially powerful, DD is problematic because apparent positive clones require time-consuming verification which may be made even more difficult if only small amounts of starting material are available. We have devised a screening approach to address these issues in primary human hematopoietic cells. Candidate clones are identified in a slot-blot format and verified by 'Virtual Northern' blot analyses using globally amplified cDNA as the verification probe. This method is fast, and since it requires only 0.2 μg of total RNA, it is particularly useful when only limited amounts of study tissue are available.

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Author notes

  1. H-L Hung

    Present address: Division of Hematology, Children's Hospital of Philadelphia, Philadelphia, PA, USA

  2. F Song

    Present address: Mt Sinai Medical Center, New York, NY, USA

Authors and Affiliations

  1. Divisions of Hematology/Oncology, Department of Pathology and Laboratory Medicine, and Hematology/Molecular Diagnosis, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    H-L Hung & A Gewirtz

  2. Colgate University, Hamilton, N York, USA

    A Gewirtz

Authors
  1. H-L Hung
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  2. F Song
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  3. A Gewirtz
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Divisions of Hematology/Oncology, Department of Medicine and Hematology/Molecular Diagnosis, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

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Hung, HL., Song, F. & Gewirtz, A. A method for identifying differentially expressed genes in rare populations of primary human hematopoietic cells. Leukemia 13, 295–297 (1999). https://doi.org/10.1038/sj.leu.2401274

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  • DOI: https://doi.org/10.1038/sj.leu.2401274

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