Abstract
We studied the functional characteristics of subpopulations of cord blood-derived CD34+ cells expressing different levels of CD38 and c-kit antigens, using clonal cell culture and long-term culture with allogeneic bone marrow stromal cells or the MS-5 murine stromal cell line to assay long-term culture-initiating cells (LTC-IC) in each subpopulation. To investigate the capacity for replication, proliferation, and differentiation of each subpopulation of CD34+ cells, we also studied the correlation between LTC-IC and telomerase activity. After 5 weeks of coculture, LTC-IC accounted for one out of 32 CD34+CD38− cells and one out of 33 CD34+c-kit− cells. In contrast, the frequency of LTC-IC was low in their antigen-positive counterparts (one per 84 CD34+CD38+ cells, one per 90 CD34+c-kitlow cells, and very low among CD34+c-kithigh cells). It was noteworthy that some LTC-IC derived from CD34+CD38− as well as CD34+c-kit− cells generated colony-forming cells (CFCs) after up to 9 weeks of coculture. Telomerase activity was consistently low in CD34+CD38− and CD34+c-kit− cells compared to CD38+ or c-kithigh or low cells, suggesting that CD34+CD38− or c-kit− cells are likely to be more quiescent. These results suggest that the CD34+CD38− and CD34+c-kit− cell populations are primitive stem/progenitor cells, and that the telomerase activity of these cells correlates with their proliferative capacity as well as their stage of differentiation.
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Sakabe, H., Yahata, N., Kimura, T. et al. Human cord blood-derived primitive progenitors are enriched in CD34+c-kit− cells: correlation between long-term culture-initiating cells and telomerase expression. Leukemia 12, 728–734 (1998). https://doi.org/10.1038/sj.leu.2401001
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DOI: https://doi.org/10.1038/sj.leu.2401001
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