Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Stem Cell Analysis

Correlation between serum lactate dehydrogenase and stem cell mobilization

Bone Marrow Transplantation volume 40, pages 931–934 (2007)Cite this article

Abstract

After observing a correlation between elevated serum lactate dehydrogenase (LDH) levels and good stem cell collections, retrospective data from 540 donors undergoing 650 stem cell apheresis procedures (87% autologous, 13% allogeneic) were studied to determine the correlation between preapheresis LDH levels and the absolute peripheral blood CD34+ cell count (PBCD34). PBCD34 (1–1611/μl; median 40) correlated modestly with leukocytes (0.5–118.2 × 109/l; median 30.2) (r=0.16; P=0.00005) and poorly with platelets (16–660 × 109/l; median 131) (r=0.02; P=0.69). The correlation between LDH (64–1664 IU/l; median 310) and PBCD34 was very strong (r=0.54; P<10−48). In multivariate regression analysis, serum LDH was the only factor correlating significantly with PBCD34. The correlation between serum LDH and PBCD34 was strong on the first day of collection (n=517; r=0.53; P<10−37), weakened on the second day (n=74; r=0.37; P=0.0009) and disappeared beyond day 2 (n=59; r=0.09; P=0.49). PBCD34 was significantly higher (median 53 versus median 11; P<0.00001) when LDH was elevated (n=511) compared to when LDH was normal (n=139). The relationship between serum LDH and PBCD34 was strong for autologous (r=0.54) as well as for allogeneic (r=0.41) collections. Our data suggest that it is reasonable to assume good stem cell mobilization and start apheresis if the LDH is elevated.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

Similar content being viewed by others

References

  1. Mehta J, Oyama Y, Winter J, Williams S, Tallman M, Singhal S et al. CD34+ cell collection efficiency does not correlate with the pre-leukapheresis hematocrit. Bone Marrow Transplant 2001; 28: 597–601.

    Article  CAS  PubMed  Google Scholar 

  2. Mehta J, Singhal S, Gordon L, Tallman M, Williams S, Luyun R et al. Cobe Spectra is superior to Fenwal CS 3000 Plus for collection of hematopoietic stem cells. Bone Marrow Transplant 2002; 29: 563–567.

    Article  CAS  PubMed  Google Scholar 

  3. Gidron A, Verma A, Doyle M, Boggio L, Evens A, Gordon L et al. Can the stem cell mobilization technique influence CD34+ cell collection efficiency of leukapheresis procedures in patients with hematologic malignancies? Bone Marrow Transplant 2005; 35: 243–246.

    Article  CAS  PubMed  Google Scholar 

  4. Tomblyn M, Gordon LI, Singhal S, Tallman MS, Williams S, Winter JN et al. Use of total leukocyte and platelet counts to guide stem cell apheresis in healthy allogeneic donors treated with G-CSF. Bone Marrow Transplant 2005; 36: 663–666.

    Article  CAS  PubMed  Google Scholar 

  5. Singhal S, Mehta J, Desikan K, Siegel D, Singh J, Munshi N et al. Collection of peripheral blood stem cells after a preceding autograft: unfavorable effect of prior interferon-α therapy. Bone Marrow Transplant 1999; 24: 13–17.

    Article  CAS  PubMed  Google Scholar 

  6. Kessinger A, Sharp JG . The whys and hows of hematopoietic progenitor and stem cell mobilization. Bone Marrow Transplant 2003; 31: 319–329.

    Article  CAS  PubMed  Google Scholar 

  7. To LB, Bashford J, Durrant S, MacMillan J, Schwarer AP, Prince HM et al. Successful mobilization of peripheral blood stem cells after addition of ancestim (stem cell factor) in patients who had failed a prior mobilization with filgrastim (granulocyte colony-stimulating factor) alone or with chemotherapy plus filgrastim. Bone Marrow Transplant 2003; 31: 371–378.

    Article  CAS  PubMed  Google Scholar 

  8. Ford CD, Green W, Warenski S, Petersen FB . Effect of prior chemotherapy on hematopoietic stem cell mobilization. Bone Marrow Transplant 2004; 33: 901–905.

    Article  CAS  Google Scholar 

  9. Morgan SJ, Seymour JF, Grigg A, Matthews JP, Prince HM, Wolf MM et al. Predictive factors for successful stem cell mobilization in patients with indolent lymphoproliferative disorders previously treated with fludarabine. Leukemia 2004; 18: 1034–1038.

    Article  CAS  PubMed  Google Scholar 

  10. Aliberti G, Pulignano I, Proietta M, Corvisieri P, de Michele LV . Lactate dehydrogenase and its isoenzymes in the marrow and peripheral blood from haematologically normal subjects. Physiol Res 1997; 46: 435–438.

    CAS  PubMed  Google Scholar 

  11. Maiche AG, Muhonen T, Porkka K . Lactate dehydrogenase changes during granulocyte colony-stimulating factor treatment. Lancet 1992; 340: 853.

    Article  CAS  PubMed  Google Scholar 

  12. Pan L, Beverley PC, Isaacson PG . Lactate dehydrogenase (LDH) isoenzymes and proliferative activity of lymphoid cells—an immunocytochemical study. Clin Exp Immunol 1991; 86: 240–245.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Hematology/Oncology, Hematopoietic Stem Cell Transplant Program, Feinberg School of Medicine, The Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA

    K Egan, V Singh, A Gidron & J Mehta

Authors
  1. K Egan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A Gidron
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J Mehta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J Mehta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Egan, K., Singh, V., Gidron, A. et al. Correlation between serum lactate dehydrogenase and stem cell mobilization. Bone Marrow Transplant 40, 931–934 (2007). https://doi.org/10.1038/sj.bmt.1705851

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.bmt.1705851

Keywords

This article is cited by

Search

Advanced search

Quick links