Abstract
We analyzed donor-type chimerism in CD3+, CD14.15+ and CD56+ cells from 36 patients who had undergone conventional-intensity allogeneic stem cell transplantation (CST) and 34 patients who had undergone nonmyeloablative allogeneic stem cell transplantation (NST) for hematological malignancies. On day 28 after transplantation, all fractions in NST patients and CD3+ cells in CST patients who received a non-total body irradiation (TBI) regimen showed more frequent mixed chimerism (<90% donor cells) than those in patients who had received TBI. NST patients with acute graft-versus-host disease (grade II–IV) frequently showed more than 50% donor-type chimerism in CD3+ cells on day 14 (P=0.029). NST patients with <50% donor-type chimerism on day 14 and with <90% donor-type chimerism on day 28 in CD56+ cells had significantly poor 1-year overall survival (0 vs 91%, P<0.001 and 20 vs 74%, P=0.002, respectively). Both NST and CST patients with <90% donor-type chimerism in CD14.15+ cells on day 28 had significantly poor 1-year overall survival (14 vs 70%, P=0.005 and 0 vs 66%, P=0.002, respectively). Our data show that the extent of donor-type chimerism in lineage-specific cells appears to have an impact on outcome after allogeneic stem cell transplantation.
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Acknowledgements
We thank all the physicians and nursing staff of the HSCT team and Ms M Yamane, Ms M Mayanagi and Y Ishimaru for their technical assistance. This work was supported in part by a grant from the Idiopathic Hematological Disease and Bone Marrow Transplantation Research Committee of the Ministry of Health and Welfare of Japan and by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
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Miura, Y., Tanaka, J., Toubai, T. et al. Analysis of donor-type chimerism in lineage-specific cell populations after allogeneic myeloablative and nonmyeloablative stem cell transplantation. Bone Marrow Transplant 37, 837–843 (2006). https://doi.org/10.1038/sj.bmt.1705352
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DOI: https://doi.org/10.1038/sj.bmt.1705352
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