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Myeloid Reconstitution

Serum stem cell growth factor for monitoring hematopoietic recovery following stem cell transplantation

Bone Marrow Transplantation volume 32, pages 391–398 (2003)Cite this article

Summary:

Stem cell growth factor (SCGF) is a novel cytokine for primitive hematopoietic progenitor cells. Although it has burst-promoting activity and granulocyte/macrophage colony-promoting activity in vitro, its significance in hematopoiesis in vivo has not been elucidated. In this study, we have established enzyme-linked immunosorbent assay (ELISA) to quantify human SCGF and measured serum cytokines in normal volunteers and 27 patients undergoing stem cell transplantation (SCT), including six autologous and 21 allogeneic transplants. SCGF levels gradually increased after SCT regardless of graft-versus-host disease or type of transplant. The maximum level of SCGF was observed during the rapid granulocyte recovery phase in patients subjected to an autologous transplantation, and during the granulocyte stabilization phase in allogeneic patients. SCGF levels in PBSCT patients began to rise earlier than in BMT patients. Two patients with no increment of SCGF after SCT showed delayed engraftment. The source of SCGF was further analyzed by RT-PCR and we found that SCGF was highly expressed in bone marrow (BM) CD34+ and CD34−CD33+ cells, but not in BM CD34−CD33− cells, BM stromal cells and peripheral blood cells. The cell population expressing SCGF in BM possess the colony-forming cell activity. Therefore, serum SCGF can be an indicator of hematopoietic recovery following SCT.

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References

  1. Hiraoka A, Sugimura A, Seki T et al. Cloning, expression, and characterization of a cDNA encoding a novel human growth factor for primitive hematopoietic progenitor cells. Proc Natl Acad Sci USA 1997; 94: 7577–7582.

    Article  CAS  Google Scholar 

  2. Hiraoka A, Ohkubo T, Fukuda M . Production of human hematopoietic survival and growth factor by a myeloid leukemia cell line (KPB-M15) and placenta as detected by a monoclonal antibody. Cancer Res 1987; 47: 5025–5030.

    CAS  PubMed  Google Scholar 

  3. Hiraoka A, Yano K, Kagami N et al. Stem cell growth factor: in situ hybridization analysis on the gene expression, molecular characterization and in vitro proliferative activity of a recombinant preparation on primitive hematopoietic progenitor cells. Hematol J 2001; 2: 307–315.

    Article  CAS  Google Scholar 

  4. Weinthal JA . The role of cytokines following bone marrow transplantation: indications and controversies. Bone Marrow Transplant 1996; 18 (Suppl. 3): S10–S14.

    PubMed  Google Scholar 

  5. Ogawa M . Differentiation and proliferation of hematopoietic stem cells. Blood 1993; 81: 2844–2853.

    CAS  Google Scholar 

  6. Rowbottom AW, Riches PG, Downie C, Hobbs JR . Monitoring cytokine production in peripheral blood during acute graft-versus-host disease following allogeneic bone marrow transplantation. Bone Marrow Transplant 1993; 12: 635–641.

    CAS  Google Scholar 

  7. Imamura M, Hashino S, Kobayashi H et al. Serum cytokine levels in bone marrow transplantation: synergistic interaction of interleukin-6, interferon-gamma, and tumor necrosis factor-alpha in graft-versus-host disease. Bone Marrow Transplant 1994; 13: 745–751.

    CAS  Google Scholar 

  8. Cairo MS, Gillan ER, Weinthal J et al. Decreased endogenous circulating steel factor (SLF) levels following allogeneic and autologous BMT: lack of an inverse correlation with post-BMT myeloid engraftment. Bone Marrow Transplant 1993; 11: 155–161.

    CAS  PubMed  Google Scholar 

  9. Weisdorf DJ, DeFor T, Nichol J et al. Thrombopoietic cytokines in relation to platelet recovery after bone marrow transplantation. Bone Marrow Transplant 2000; 25: 711–715.

    Article  CAS  Google Scholar 

  10. Hamaguchi M, Yamada H, Morishima Y et al. Serum thrombopoietin level after allogeneic bone marrow transplantation: possible correlations with platelet recovery, acute graft-versus-host disease and hepatic veno-occlusive disease. Nagoya Bone Marrow Transplantation Group. Int J Hematol 1996; 64: 241–248.

    Article  CAS  Google Scholar 

  11. Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256: 495–497.

    Article  CAS  Google Scholar 

  12. Glucksberg H, Storb R, Fefer A et al. Clinical manifestations of graft-versus-host disease in human recipients of marrow from HLA-matched sibling donors. Transplantation 1974; 18: 295–304.

    Article  CAS  Google Scholar 

  13. Takatsuka H, Takemoto Y, Yamada S et al. Complications after bone marrow transplantation are manifestations of systemic inflammatory response syndrome. Bone Marrow Transplant 2000; 26: 419–426.

    Article  CAS  Google Scholar 

  14. Molineux G, McNiece LK . Stem cell factor. In: Angus WT (ed.). The cytokine handbook. Academic Press: California, 1998, pp 713–725.

    Google Scholar 

  15. David JK . Thrombopoietin. In: Joost JO, Marc F(eds.). Cytokine reference. Academic Press: California, 2001, pp 965–982.

    Google Scholar 

  16. Rusten LS, Lyman SD, Veuby OP et al. The FLT3 ligand is a direct and potent stimulator of the growth of primitive and committed human CD34+ bone marrow progenitor cells in vitro. Blood 1996; 87: 1317–1325.

    CAS  Google Scholar 

  17. Steffen M, Pichlmeier U, von dem Busche C, Zander A . Serum levels of stem cell factor in patients during transplantation of bone marrow or peripheral blood stem cells. J Hematother Stem Cell Res 2000; 9: 55–61.

    Article  CAS  Google Scholar 

  18. Steffen M, Durken M, Pichlmeier U et al. Serum interleukin-6 levels during bone marrow transplantation: impact on transplant-related toxicity and engraftment. Bone Marrow Transplant 1996; 18: 301–307.

    CAS  Google Scholar 

Download references

Acknowledgements

We thank Drs Takashi Yahata, Hideyuki Matsuzawa and Yoshihiko Nakamura for technical assistance, members of Department of Hematology/Oncology of Tokai University for management of clinical samples.

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Authors and Affiliations

  1. Department of Hematology, Oncology and Rheumatology, Tokai University School of Medicine, Kanagawa, Japan

    C Ito, K Ando, S Watanabe, F Yoshiba, K Kishi & T Hotta

  2. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., LTD., Tokyo, Japan

    H Sato, A Furuya, K Shitara & S Sugimoto

  3. Kyowa Medex Co., LTD., Tokyo, Japan

    H Kohno

  4. Department of Internal Medicine, Osaka Dental University, Osaka, Japan

    A Hiraoka

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  1. C Ito
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  2. H Sato
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Ito, C., Sato, H., Ando, K. et al. Serum stem cell growth factor for monitoring hematopoietic recovery following stem cell transplantation. Bone Marrow Transplant 32, 391–398 (2003). https://doi.org/10.1038/sj.bmt.1704152

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  • DOI: https://doi.org/10.1038/sj.bmt.1704152

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