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Progenitor Cell Mobilisation

Influence of preapheresis clinical factors on the efficiency of CD34+ cell collection by large-volume apheresis

Bone Marrow Transplantation volume 31, pages 851–855 (2003)Cite this article

Summary:

We evaluated 120 leukapheresis procedures (93 patients), in order to detect clinical factors that influence the efficiency of CD34+ collection using Cobe Spectra™ cell separators. Hematocrit was >27% and platelet count >30 000/μl in >95% of patients. Platelet transfusions were given if the postprocedure count was <20 000/μl. Multiple regression analysis was used to analyze putative factors, and a predictive equation defined by stepwise regression modeling. The mean efficiency was 0.59 (s.d. 0.27). Sex (M>F; P=0.01), the volume processed (inver-sely; P=0.01) and CD34+ cell count (inversely; P=0.04) were associated with efficiency, whereas hematocrit, platelet or leukocyte count, catheter type and patient weight were not. The effect size for predictive factors was small (R2=0.21). Adverse events were limited to hypocalcemia. We conclude that female sex, volume processed and CD34+ cell count adversely influence the efficiency of CD34+ cell leukapheresis. However, the impact of volume and CD34+ cell count is small, and likely to be offset by the influence of these same factors on overall yield. Leukapheresis appears to be safe and efficient for autologous blood and marrow transplantation patients with hematocrit >27% and platelet count >30 000/μl.

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Acknowledgements

We thank Linda Kellner-Jenkins and Debbe Horsey (apheresis nurses) and the staff of the hematology laboratory, Greenebaum Cancer Center, for their help in obtaining the data for his study. This study was supported in part by the American Society of Hematology, through a Student Grant to IT.

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  1. University of Maryland School of Medicine, Greenebaum Cancer Center, Baltimore, MD, USA

    C Sarkodee-Adoo, I Taran, C Guo, F Buadi, R Murthy, E Cox, R Lopez, S Westphal, S Shope, B O'Connell, L Wethers & B Meisenberg

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  1. C Sarkodee-Adoo
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Sarkodee-Adoo, C., Taran, I., Guo, C. et al. Influence of preapheresis clinical factors on the efficiency of CD34+ cell collection by large-volume apheresis. Bone Marrow Transplant 31, 851–855 (2003). https://doi.org/10.1038/sj.bmt.1704034

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