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Graft-Versus-Host Disease

T cell infiltration and chemokine expression: relevance to the disease localization in murine graft-versus-host disease

Bone Marrow Transplantation volume 29, pages 979–986 (2002)Cite this article

Abstract

Acute graft-versus-host disease (GVHD) involves mainly skin, liver and intestines. Other organs such as heart, muscle and central nervous system are seldom affected, although their parenchymal cells also express alloantigens, such as MHC class I antigens. The mechanism of this selective involvement of distinct organs in acute GVHD is not well understood. We postulated that it might be related to the selective migration of activated alloreactive T cells. Indeed, T cell infiltration, revealed by examination of serial samples using flow cytometry and immunohistology, occurred early and continuously in the target organs such as the liver, but not in a non-target organ, the heart, in a murine acute GVHD model. Since T cell migration is largely controlled by the expression of chemokine and chemokine receptors, we investigated the chemokine spectrum in target/non-target organs of mice with acute GVHD. We found that in the spleen and liver MIP-1α, MIP-2 and Mig were the predominant chemokines expressed. In another target organ, the skin, MIP-1α, MIP-2, MCP-1 and MCP-3 were all highly expressed. In a non-target organ of acute GVHD, the heart, the predominant chemokines expressed were MCP-1 and MCP-3. This distinct pattern of chemokine expression in these organs may contribute to the preferential recruitment of inflammatory cells into the liver and skin, but not into the heart, in acute GVHD.

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Acknowledgements

This work was supported by grants 30030, NMRC/0348/1999, NMRC/0327/1999 (HZH) from the National Medical Research Council, Singapore, and a grant from Leukemia Research Foundation (HZH).

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Authors and Affiliations

  1. Departments of Microbiology, Faculty of Medicine, National University of Singapore, Republic of Singapore

    JY New, B Li, WP Koh, HK Ng, SY Tan, EH Yap, SH Chan & HZ Hu

  2. Department of Surgery, University of Wisconsin, Madison, WI, USA

    HZ Hu

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  1. JY New
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New, J., Li, B., Koh, W. et al. T cell infiltration and chemokine expression: relevance to the disease localization in murine graft-versus-host disease. Bone Marrow Transplant 29, 979–986 (2002). https://doi.org/10.1038/sj.bmt.1703563

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