Abstract
Peripheral blood stem cell (PBSC) harvesting in the smallest children (weight <10 kg) using separators is complicated by specific problems. The volume of the separation set exceeds 25% of the total blood volume and the vascular access is generally not sufficient. Therefore, a simple manual technique for PBSC harvesting was developed. Three children (6–9 kg), with newly diagnosed tumours were scheduled to be treated with three to six sequential courses of high-dose chemotherapy, each followed by PBSC support. PBSC harvests were started after mobilization using cyclophosphamide and G-CSF when the peripheral blood CD34+ cell count exceeded 50/μl. About 50 ml of blood was drawn from a venous catheter, injected into a transfer bag containing ACD-A, and centrifuged. The buffy coat obtained was pooled in a collection bag, remaining plasma and erythrocytes were immediately reinfused and a subsequent cycle started. From three to 13 cycles were performed in 1–3 days and 18.0–32.2 × 106 CD34+cells/kg were collected. We did not detect any bacterial contamination or any notable complications. Fifteen PBSC reinfusions have been performed to date, each with rapid engraftment taking between 7 and 13 days. Patients are in very good PR (18 months from diagnosis) or in CR (6 and 8 months). We can conclude that this procedure is feasible and safe.
Bone Marrow Transplantation (2002) 29, 57–61. doi:10.1038/sj.bmt.1703334
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This work was supported in part by the Research Grant of the Ministry of Health of the Czech Republic.
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Kořístek, Z., Štěrba, J., Havranová, D. et al. Technique for PBSC harvesting in children of weight under 10 kg. Bone Marrow Transplant 29, 57–61 (2002). https://doi.org/10.1038/sj.bmt.1703334
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DOI: https://doi.org/10.1038/sj.bmt.1703334
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