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Cord Blood Stem Cell Transplants

Cord blood transplants: early recovery of neutrophils from co-transplanted sibling haploidentical progenitor cells and lack of engraftment of cultured cord blood cells, as ascertained by analysis of DNA polymorphisms

Bone Marrow Transplantation volume 28, pages 355–363 (2001)Cite this article

Abstract

The number of infused cells is a very important factor in cord blood transplant (CBT) engraftment. Prior ex vivo expansion of aliquots of transplanted cord blood (CB) units is being investigated as a procedure to increase engraftment potential, but results are difficult to evaluate due to a lack of markers for assessing the contribution of expanded cells. We transplanted five patients, infusing the best available CB unit and cells from a second donor simultaneously. In two patients, these cells were obtained from another frozen CB unit by CD34+positive selection and culture expansion; the other three patients received uncultured highly purified haploidentical CD34+ cells. The first two patients had DNA from the culture expanded CB cells detected only for a few days around day +11 when the absolute neutrophil count (ANC) was >200/μl; thereafter and when the ANC was <500/μl, only donor DNA from the uncultured CB was detected. For the other three patients, DNA analysis showed early and transient granulocyte engraftment of haploidentical cells, progressively replaced by the CB-derived granulocytes. We concluded that: (1) simultaneous infusion of lymphocyte-depleted HLA highly mismatched haematopoietic progenitor cells has not produced unfavourable effects for CBT; (2) the double transplant model is suitable for evaluating the engraftment potential of ex vivocultured CB cells in the clinical setting; (3) the culture conditions used did not result in early recovery of ANC; and (4) co-transplantation of purified uncultured HLA haploidentical CD34+ cells may reduce the time of neutropenia following CBT.Bone Marrow Transplantation (2001) 28, 355–363.

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Acknowledgements

We wish to thank the ‘Fundación Ramón Areces’ and the ‘Fundación Josep Carreras’ for financial support of this work. Mrs P Dodi is acknowledged for her skilled editorial help.

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Authors and Affiliations

  1. Servicio de Hematología, Clínica Puerta de Hierro, Universidad Autónoma de Madrid, Spain

    MN Fernández, C Regidor, R Cabrera, J García-Marco, M Briz, R Forés & I Sanjuán

  2. Banco de Sangre de Cordón de Barcelona (IRO), Spain

    S Querol & J García

  3. The Anthony Nolan Research Institute and Royal Free Hospital School of Medicine, London, UK

    A McWhinnie & A Madrigal

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  1. MN Fernández
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Fernández, M., Regidor, C., Cabrera, R. et al. Cord blood transplants: early recovery of neutrophils from co-transplanted sibling haploidentical progenitor cells and lack of engraftment of cultured cord blood cells, as ascertained by analysis of DNA polymorphisms. Bone Marrow Transplant 28, 355–363 (2001). https://doi.org/10.1038/sj.bmt.1703143

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