Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

CD34+ Cell Selection

Combined positive and negative cell selection from allogeneic peripheral blood progenitor cells (PBPC) by use of immunomagnetic methods

Bone Marrow Transplantation volume 27, pages 683–687 (2001)Cite this article

Abstract

Twenty-four mobilized peripheral blood products from healthy donors for allogeneic transplantation were positively selected for CD34+ cells and depleted of CD4+ and CD8+ cells (+/− selection) by combining clinical grade immunomagnetic methods. A sequential, ‘two-step’ strategy combining positive selection of CD34+ cells by use of the Isolex 300i (versions 1 and 2) device and T cell depletion (TCD) using the MaxSep device and a simultaneous, ‘one-step’ method of CD34+cell selection and TCD using the Isolex 300i (software versions 1 and 2) have been investigated. Using these magnetic bead separation systems, two groups of sequential +/− selection (Isolex 300i version 1/MaxSep and Isolex 300i version 2/MaxSep) and two groups of simultaneous +/− selection (Isolex 300i versions 1 and 2) were analysed. In the sequential +/− selection, logarithms of TCD (CD3+ cell depletion) obtained by the positive selection step had median values of 3.7 with the version 1 (n = 5) and 4.5 with version 2 software of the Isolex 300i (n = 5) (P = 0.07). Version 2 also gave a higher CD34+ cell purity and yield than did version 1 (92% vs 77%, P < 0.05 and 55% vs 34%, P = 0.3, respectively). Additional TCD obtained in the second step with the MaxSep device for the two groups had a median value of 0.9 log and 7% CD34+cell losses. In the simultaneous +/− selection, the Isolex 300i version 2 (n = 10) gave a median TCD of 5.1 log and version 1 (n = 4) of 4 log (P < 0.005). Higher CD34+cell purity and yield were also obtained with version 2 than with version 1 (97% and 76%, P < 0.005 and 57% and 39%, P = 0.07, respectively). These data indicate that simultaneous, ‘one-step’ +/− selection in the Isolex 300i version 2 achieves a high TCD with a high CD34+ cell purity and an acceptable CD34+ cell yield. Bone Marrow Transplantation (2001) 27, 683–687.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Ringden O, Nilsson B . Death by graft-versus-host disease associated with HLA mismatch, high recipient age, low marrow cell dose, and splenectomy Transplantation 1985 40: 39–44

    Article  CAS  PubMed  Google Scholar 

  2. Aversa F, Tabilio A, Velardi A et al. Treatment of high-risk acute leukemia with T-cell-depleted stem cells from related donors with one fully mismatched HLA haplotype New Engl J Med 1998 339: 1186–1193

    Article  CAS  PubMed  Google Scholar 

  3. Muller S, Schulz A, Reiss U et al. Definition of a critical T cell threshold for prevention of GVHD after HLA non-identical PBPC transplantation in children Bone Marrow Transplant 1999 24: 575–581

    Article  CAS  PubMed  Google Scholar 

  4. Berenson RJ, Bensinger WI, Hill RS et al. Engraftment after infusion of CD34+ marrow cells in patients with breast cancer or neuroblastoma Blood 1991 77: 1717–1722

    CAS  PubMed  Google Scholar 

  5. Martín-Henao GA, Inglés-Esteve J, Cancelas JA, Garcia J . Isolation of CD34+ hematopoietic progenitor cells in chronic myeloid leukemia by magnetic activated cell sorting (MACS) Bone Marrow Transplant 1996 18: 603–609

    PubMed  Google Scholar 

  6. Cancelas JA, Querol S, Canals C et al. Peripheral blood CD34+ cell immunomagnetic selection in breast cancer patients: effect on hematopoietic progenitor content and hematologic recovery after high-dose chemotherapy and autotransplantation Transfusion 1998 38: 1063–1070

    Article  CAS  PubMed  Google Scholar 

  7. Urbano-Ispizua A, Solano C, Brunet S et al. Allogeneic transplantation of selected CD34+ cells from peripheral blood: experience of 62 cases using immunoadsorption or immunomagnetic technique. Spanish Group of Allo-PBT Bone Marrow Transplant 1998 22: 519–525

    Article  CAS  PubMed  Google Scholar 

  8. Tseng-Law J, Szalay P, Guillermo R et al. Identification of a peptide directed against the anti-CD34 antibody, 9C5, by phage display and its use in hematopoietic stem cell selection Exp Hematol 1999 27: 936–945

    Article  CAS  PubMed  Google Scholar 

  9. Martín-Henao GA, Picón M, Amill B et al. Isolation of CD34+ progenitor cells from peripheral blood by use of an automated immunomagnetic selection system: factors affecting the results Transfusion 2000 40: 35–43

    Article  PubMed  Google Scholar 

  10. Atkinson K, Farrelly H, Cooley M et al. Human marrow T cell dose correlates with severity of subsequent acute graft-versus-host disease Bone Marrow Transplant 1987 2: 51–57

    CAS  PubMed  Google Scholar 

  11. Kernan NA, Collins NH, Juliano L et al. Clonable T lymphocytes in T cell-depleted bone marrow transplants correlate with development of graft-v-host disease Blood 1986 68: 770–773

    CAS  PubMed  Google Scholar 

  12. Lowenberg B, Wagemaker G, van Bekkum DW et al. Graft-versus-host disease following transplantation of ‘one log’ versus ‘two log’ T-lymphocyte-depleted bone marrow from HLA-identical donors Bone Marrow Transplant 1986 1: 133–140

    CAS  PubMed  Google Scholar 

  13. Verdonck LF, Dekker AW, de Gast GC et al. Allogeneic bone marrow transplantation with a fixed low number of T cells in the marrow graft Blood 1994 83: 3090–3096

    CAS  PubMed  Google Scholar 

  14. Querol S, Capmany G, Azqueta C et al. Direct immunomagnetic method for CD34+ cell selection from cryopreserved cord blood grafts for ex vivo expansion protocols Transfusion 2000 40: 625–631

    Article  CAS  PubMed  Google Scholar 

  15. Dreger P, Viehmann K, Steinmann J et al. G-CSF-mobilized peripheral blood progenitor cells for allogeneic transplantation: comparison of T cell depletion strategies using different CD34+ selection systems or CAMPATH-1 Exp Hematol 1995 23: 147–154

    CAS  PubMed  Google Scholar 

  16. Rambaldi A, Borleri G, Dotti G et al. Innovative two-step negative selection of granulocyte colony-stimulating factor-mobilized circulating progenitor cells: adequacy for autologous and allogeneic transplantation Blood 1998 91: 2189–2196

    CAS  PubMed  Google Scholar 

  17. Bertolini F, Thomas T, Battaglia M et al. A new ‘two step’ procedure for 4.5 log depletion of T and B cells in allogeneic transplantation and of neoplastic cells in autologous transplantation Bone Marrow Transplant 1997 19: 615–619

    Article  CAS  PubMed  Google Scholar 

  18. Martín-Hernandez MP, Arrieta R, Martínez A et al. Haploidentical peripheral blood stem cell transplantation with a combination of CD34 selection and T cell depletion as graft-versus-host disease prophylaxis in a patient with severe combined immunodeficiency Bone Marrow Transplant 1997 20: 797–799

    Article  PubMed  Google Scholar 

  19. Schumm M, Lang P, Taylor G et al. Isolation of highly purified autologous and allogeneic peripheral CD34+ cells using the CliniMACS device J Hematother 1999 8: 209–218

    Article  CAS  PubMed  Google Scholar 

  20. McNiece I, Briddell R, Stoney G et al. Large-scale isolation of CD34+ cells using the Amgen cell selection device results in high levels of purity and recovery J Hematother 1997 6: 5–11

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work has been partially supported by grant SAF98-0050. Reagents used in the procedures of simultaneous positive– negative selection were kindly supplied by Baxter Healthcare.

Author information

Authors and Affiliations

  1. Department of Cryobiology and Cell Therapy, Cancer Research Institute, Barcelona, Spain

    GA Martín-Henao, M Picón, B Amill, S Querol & J García

  2. Hospital Durán i Reynals, Barcelona, Spain

    C Ferrà & A Grañena

Authors
  1. GA Martín-Henao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M Picón
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B Amill
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S Querol
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C Ferrà
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A Grañena
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J García
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martín-Henao, G., Picón, M., Amill, B. et al. Combined positive and negative cell selection from allogeneic peripheral blood progenitor cells (PBPC) by use of immunomagnetic methods. Bone Marrow Transplant 27, 683–687 (2001). https://doi.org/10.1038/sj.bmt.1702860

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.bmt.1702860

Keywords

This article is cited by

Search

Advanced search

Quick links