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Chimerism

Prognostic value of hematopoietic chimerism in patients with acute leukemia after allogeneic bone marrow transplantation: a prospective study

Bone Marrow Transplantation volume 26, pages 327–332 (2000)Cite this article

Abstract

Hematopoietic chimerism as a predictive marker for the relapse of acute leukemia after allogeneic BMT was evaluated in a prospective study. Monthly assays of hematopoietic chimerism were performed from peripheral blood samples by PCR amplification of short tandem repeats or amelogenin loci. Between December 1997 and June 1999, 33 patients enrolled and 30 were evaluable (two early deaths, one lack of informative bands for chimerism evaluation). There were 14 male and 16 female patients (15 AML and 15 ALL) with a median age of 31 years (range 16–46). Mixed chimerism (MC) was observed at least once in 14 of 30 patients (47%). There was no significant difference between 14 patients who showed MC (MC group) and 16 patients who did not show MC (complete chimerism (CC) group) in terms of age, sex, disease status at BMT, donor type, and the number of bone marrow cells infused. There was no significant difference in the neutrophil and platelet engraftment rates between the two groups. After a median follow up of 10.9 months (range 4.3–22.4), five patients in the CC group and two patients in the MC group relapsed (P = 0.27). All five patients who relapsed in the CC group maintained CC up to 1 month prior to clinical relapse. Our study demonstrated that the patients who showed MC post BMT did not have higher risk of relapse of acute leukemia when compared to patients who did not show MC. Sensitive PCR-based assays for hematopoietic chimerism applied on a monthly basis after allogeneic BMT could not predict relapse of acute leukemia. Bone Marrow Transplantation (2000) 26, 327–332.

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Acknowledgements

This work was supported in part by a grant (No. 98–109) from the Research Fund for the Asan Institute for Life Science.

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Authors and Affiliations

  1. Division of Oncology-Hematology, Department of Medicine, University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Korea

    S-J Choi, K-H Lee, J-H Lee, S Kim, H-J Chung, J-S Lee, S-H Kim & W-K Kim

  2. Division of Oncology-Hematology, Department of Clinical Pathology, University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Korea

    C-J Park & H-S Chi

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Choi, SJ., Lee, KH., Lee, JH. et al. Prognostic value of hematopoietic chimerism in patients with acute leukemia after allogeneic bone marrow transplantation: a prospective study. Bone Marrow Transplant 26, 327–332 (2000). https://doi.org/10.1038/sj.bmt.1702504

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