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Graft Versus Host Disease

Evaluation of CMV/human herpes virus-6 positivity in bronchoalveolar lavage fluids as early detection of acute GVHD following BMT: evidence of a significant relationship

Bone Marrow Transplantation volume 26, pages 77–81 (2000)Cite this article

Abstract

We evaluated the relationship between CMV and human herpes virus-6 (HHV-6) reactivation and the incidence of grades 2 to 4 acute GVHD post BMT. Bronchoalveolar lavage fluid (BALF) samples extracted from 54 BMT recipients on post-BMT day 35 were analyzed by PCR for detection of CMV DNA, HHV-6 DNA and CMV plus HHV-6 DNA. CMV DNA was detected in 26 patients and 13 (50%) developed grades 2 to 4 acute GVHD. Of the 28 who were CMV negative, only six (21.4%) developed grades 2 to 4 acute GVHD. HHV-6 was detected in 18 patients, and 11 (61.1%) developed grades 2 to 4 acute GVHD. Of the 36 who were HHV-6 negative, only eight (22.2%) developed grades 2 to 4 acute GVHD. CMV and HHV-6 were detected in 13 patients, and eight (61.5%) developed grades 2 to 4 acute GVHD. Of the 23 who were negative for both CMV and HHV-6, only three (13%) developed grades 2 to 4 acute GVHD. In all experiments, the difference between the groups was significant (P < 0.05, P < 0.05 and P < 0.01, respectively). we conclude that herpes virus infection, in particular cmv concurrent with hhv-6 reactivation, is predictive of moderate to severe acute gvhd. Bone Marrow Transplantation (2000) 26, 77–81.

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References

  1. Stocchi R, Szydlo R, Craddock C et al. A comparison of prophylactic vs pre-emptive ganciclovir to prevent cytomegalovirus disease after T-depleted volunteer unrelated donor bone marrow transplantation Bone Marrow Transplant 1999 23: 705–709

    Article  CAS  Google Scholar 

  2. Wilborn F, Brinkmann V, Schmidt CA et al. Herpesvirus type 6 in patients undergoing bone marrow transplantations: serologic features and detection by polymerase chain reaction Blood 1994 83: 3052–3058

    CAS  PubMed  Google Scholar 

  3. Wang FZ, Dahl H, Linde A et al. Lymphotropic herpes viruses in allogeneic bone marrow transplantation Blood 1996 88: 3615–3620

    CAS  PubMed  Google Scholar 

  4. Przepiorka D, Smith TL, Folloder J et al. Risk factors for acute graft-versus-host disease after allogeneic blood stem cell transplantation Blood 1999 94: 1465–1470

    CAS  PubMed  Google Scholar 

  5. Maltazou H, Whimbey E, Abi-Said D et al. CMV disease in adult marrow transplant recipients receiving ganciclovir prophylaxis: a retrospective study Bone Marrow Transplant 1999 24: 665–669

    Article  Google Scholar 

  6. Miller W, Flynn P, McCullough J et al. Cytomegalovirus infection after bone marrow transplantation: an association with acute graft-v-host disease Blood 1986 67: 1162–1167

    CAS  PubMed  Google Scholar 

  7. Takemoto Y, Hashimoto T, Eizuru Y et al. Rapid diagnosis of cytomegalovirus interstitial pneumonitis by the polymerase chain reaction method – comparison of four diagnostic procedures Kansenshogaku Zasshi 1993 67: 295–298

    Article  CAS  Google Scholar 

  8. Petersen FB, Buckner CD, Appelbaum FR et al. Busulfan, cyclophosphamide and fractionated total body irradiation as a preparatory regimen for marrow transplantation in patients with advanced hematological malignancies: a phase 1 study Bone Marrow Transplant 1989 4: 617–623

    CAS  PubMed  Google Scholar 

  9. Schmidt GM, Horak DA, Niland JC et al. A randomized controlled trial of prophylactic ganciclovir for cytomegalovirus pulmonary infection in recipients of allogeneic bone marrow transplants. The City of Hope-Stanford-Syntex New Engl J Med 1991 324: 1005–1011

    Article  CAS  Google Scholar 

  10. Singhal S, Metha J, Powles R et al. Three weeks of ganciclovir for cytomegaloviraemia after allogeneic bone marrow transplantation Bone Marrow Transplant 1995 15: 777–781

    CAS  PubMed  Google Scholar 

  11. Yalcin S, Karpuzoglu T, Suleymanlar G et al. Human herpes virus 6 and human herpesvirus 7 infections in renal transplant recipients and healthy adults in Turkey Arch Virol 1994 136: 183–190

    Article  CAS  Google Scholar 

  12. Maeda Y, Teshima T, Yamada M et al. Monitoring of human herpes virus after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation Br J Haematol 1999 105: 295–302

    Article  CAS  Google Scholar 

  13. Appleton AL, Sviland L, Peiris JSM et al. Role of target organ infection with cytomegalovirus in the pathogenesis of graft -versus- host disease Bone Marrow Transplant 1995 15: 557–561

    CAS  PubMed  Google Scholar 

  14. Fajac A, Stephan F, Ibraham A et al. Value of cytomegalovirus detection by PCR in bronchoalveolar lavage routinely performed in asymptomatic bone marrow recipients Bone Marrow Transplant 1997 20: 581–585

    Article  CAS  Google Scholar 

  15. Sakuma H, Hosoya M, Kanno H et al. Risk of cytomegalovirus infection after peripheral blood stem cell transplantation Bone Marrow Transplant 1997 19: 49–53

    Article  CAS  Google Scholar 

  16. Reddy V, Hao Y, Lipton J et al. Management of allogeneic bone marrow transplant recipients at risk for cytomegalovirus disease using a surveillance bronchoscopy and prolonged pre-emptive ganciclovir therapy J Clin Virol 1999 13: 149–159

    Article  CAS  Google Scholar 

  17. Weiner RS, Bortin MM, Gale RP et al. Interstitial pneumonitis after bone marrow transplantation Ann Intern Med 1986 104: 168–175

    Article  CAS  Google Scholar 

  18. Via CS, Shanley JD, Weatherly BR et al. Altered threshold for the induction of graft-versus-host immunodeficiency following murine cytomegalovirus infection. Host and donor contributions Transplantation 1988 46: 298–302

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The technical support of Miss Aki Utsumi and Miss Rie Shikita is greatly appreciated. We express our gratitude to all members and staff of BMT team in the Second Department of Internal Medicine, Hyogo College of Medicine.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan

    Y Takemoto, H Takatsuka, H Wada, A Mori, K Saheki, M Okada, S Tamura, Y Fujimori, T Okamoto & E Kakishita

  2. Third department of Internal Medicine, Kinki University of Medicine, Osaka, Japan

    A Kanamaru

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Takemoto, Y., Takatsuka, H., Wada, H. et al. Evaluation of CMV/human herpes virus-6 positivity in bronchoalveolar lavage fluids as early detection of acute GVHD following BMT: evidence of a significant relationship. Bone Marrow Transplant 26, 77–81 (2000). https://doi.org/10.1038/sj.bmt.1702459

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